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Protein

Homoserine O-acetyltransferase

Gene

metX

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the methionine biosynthesis. Catalyzes the transfer of the acetyl group from acetyl-CoA to the hydroxyl group of L-homoserine to yield O-acetyl-L-homoserine.UniRule annotation

Catalytic activityi

Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.UniRule annotation

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes O-acetyl-L-homoserine from L-homoserine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Homoserine O-acetyltransferase (metX), Homoserine O-acetyltransferase (MSMEG_6772), Homoserine O-acetyltransferase (MSMEI_6590)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes O-acetyl-L-homoserine from L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641NucleophileUniRule annotation
Active sitei327 – 3271UniRule annotation
Active sitei357 – 3571UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1651-MONOMER.
UniPathwayiUPA00051; UER00074.

Protein family/group databases

ESTHERimycs2-metx. Homoserine_transacetylase.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine O-acetyltransferaseUniRule annotation (EC:2.3.1.31UniRule annotation)
Alternative name(s):
Homoserine O-trans-acetylaseUniRule annotation
Short name:
HTAUniRule annotation
Short name:
Homoserine transacetylaseUniRule annotation
Gene namesi
Name:metXUniRule annotation
Ordered Locus Names:MSMEG_1651, MSMEI_1613
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Homoserine O-acetyltransferasePRO_1000021885Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_1651.

Structurei

3D structure databases

ProteinModelPortaliA0QSZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. HTA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DWV. Bacteria.
COG2021. LUCA.
HOGENOMiHOG000246301.
KOiK00641.
OMAiGSVGGMN.
OrthoDBiPOG091H08NY.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_00296. Homoser_O_acetyltr. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QSZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIIEERATD TGMATVPLPA EGEIGLVHIG ALTLENGTVL PDVTIAVQRW
60 70 80 90 100
GELAPDRGNV VMVLHALTGD SHVTGPAGDG HPTAGWWDGV AGPGAPIDTD
110 120 130 140 150
HWCAIATNVL GGCRGSTGPG SLAPDGKPWG SRFPQITIRD QVAADRAALA
160 170 180 190 200
ALGITEVAAV VGGSMGGARA LEWLVTHPDD VRAGLVLAVG ARATADQIGT
210 220 230 240 250
QSTQVAAIKA DPDWQGGDYH GTGRAPTEGM EIARRFAHLT YRGEEELDDR
260 270 280 290 300
FANTPQDDED PLTGGRYAVQ SYLEYQGGKL ARRFDPGTYV VLSDALSSHD
310 320 330 340 350
VGRGRGGVEA ALRSCPVPVV VGGITSDRLY PIRLQQELAE LLPGCQGLDV
360 370 380
VDSIYGHDGF LVETELVGKL IRRTLELAQR
Length:380
Mass (Da):39,954
Last modified:January 9, 2007 - v1
Checksum:i75EE16B2AEED6F98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71400.1.
CP001663 Genomic DNA. Translation: AFP38085.1.
RefSeqiWP_011727801.1. NC_008596.1.
YP_886028.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71400; ABK71400; MSMEG_1651.
AFP38085; AFP38085; MSMEI_1613.
GeneIDi4533271.
KEGGimsg:MSMEI_1613.
msm:MSMEG_1651.
PATRICi18075759. VBIMycSme59918_1636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71400.1.
CP001663 Genomic DNA. Translation: AFP38085.1.
RefSeqiWP_011727801.1. NC_008596.1.
YP_886028.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QSZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1651.

Protein family/group databases

ESTHERimycs2-metx. Homoserine_transacetylase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71400; ABK71400; MSMEG_1651.
AFP38085; AFP38085; MSMEI_1613.
GeneIDi4533271.
KEGGimsg:MSMEI_1613.
msm:MSMEG_1651.
PATRICi18075759. VBIMycSme59918_1636.

Phylogenomic databases

eggNOGiENOG4105DWV. Bacteria.
COG2021. LUCA.
HOGENOMiHOG000246301.
KOiK00641.
OMAiGSVGGMN.
OrthoDBiPOG091H08NY.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00074.
BioCyciMSME246196:GJ4Y-1651-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_00296. Homoser_O_acetyltr. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETX_MYCS2
AccessioniPrimary (citable) accession number: A0QSZ0
Secondary accession number(s): I7F941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.