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Protein

Bifunctional protein FolD

Gene

folD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192NADPUniRule annotation1
Binding sitei233NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 167NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:MSMEG_1647, MSMEI_1607
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003058442 – 283Bifunctional protein FolDAdd BLAST282

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_1647

Structurei

3D structure databases

ProteinModelPortaliA0QSY5
SMRiA0QSY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0 Bacteria
COG0190 LUCA
HOGENOMiHOG000218242
KOiK01491
OMAiAGKLCGD
OrthoDBiPOG091H0041

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 1 hit
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QSY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAISLDGKT TRDEIFVDLK ERVAALTAAG RTPGLGTVLV GDDPGSQAYV
60 70 80 90 100
RGKHADCAKV GINSIRRDLP ADITTEQLNE TIDELNANPD CTGYIVQLPL
110 120 130 140 150
PKHLDENAAL ERIDPAKDAD GLHPTNLGRL VLGKQAALPC TPRGIVHLLR
160 170 180 190 200
RFDVPIAGAH VVVIGRGVTV GRPMGLLLTR RSENATVTLC HTGTRDLPAL
210 220 230 240 250
TRQADIIIAA VGVPHMVTAD MVKPGAAVVD VGVSRVDGKL TGDVAPDVWE
260 270 280
VAGHVSPNPG GVGPLTRAFL LTNVVEAEES KLA
Length:283
Mass (Da):29,693
Last modified:January 9, 2007 - v1
Checksum:i2BC525A2E632AE11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK69727.1
CP001663 Genomic DNA Translation: AFP38080.1
RefSeqiWP_003893053.1, NZ_CP009494.1
YP_886023.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK69727; ABK69727; MSMEG_1647
AFP38080; AFP38080; MSMEI_1607
GeneIDi4536006
KEGGimsb:LJ00_08220
msg:MSMEI_1607
msm:MSMEG_1647
PATRICifig|246196.19.peg.1631

Similar proteinsi

Entry informationi

Entry nameiFOLD_MYCS2
AccessioniPrimary (citable) accession number: A0QSY5
Secondary accession number(s): I7F936
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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