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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:MSMEG_1524, MSMEI_1488
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002968351 – 350DNA-directed RNA polymerase subunit alphaAdd BLAST350

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiA0QSL8, 2 interactors
STRINGi246196.MSMEG_1524

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Beta strandi14 – 26Combined sources13
Helixi31 – 45Combined sources15
Beta strandi48 – 58Combined sources11
Beta strandi60 – 64Combined sources5
Beta strandi69 – 71Combined sources3
Helixi73 – 81Combined sources9
Beta strandi85 – 87Combined sources3
Beta strandi93 – 106Combined sources14
Helixi107 – 109Combined sources3
Beta strandi116 – 119Combined sources4
Beta strandi124 – 128Combined sources5
Beta strandi133 – 143Combined sources11
Beta strandi145 – 147Combined sources3
Turni152 – 154Combined sources3
Beta strandi166 – 168Combined sources3
Beta strandi171 – 180Combined sources10
Beta strandi184 – 186Combined sources3
Beta strandi190 – 198Combined sources9
Beta strandi200 – 202Combined sources3
Helixi204 – 218Combined sources15
Helixi252 – 254Combined sources3
Helixi258 – 267Combined sources10
Helixi272 – 277Combined sources6
Helixi280 – 284Combined sources5
Helixi291 – 302Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TW1X-ray2.76A/B/T1-350[»]
5VI5X-ray3.20A/B1-350[»]
5VI8X-ray2.76A/B1-350[»]
T251-350[»]
ProteinModelPortaliA0QSL8
SMRiA0QSL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 226Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST226
Regioni241 – 350Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST110

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF Bacteria
COG0202 LUCA
HOGENOMiHOG000218480
KOiK03040
OMAiLMKFRNF
OrthoDBiPOG091H001Z

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

A0QSL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISQRPTLS EETVAENRSR FVIEPLEPGF GYTLGNSLRR TLLSSIPGAA
60 70 80 90 100
VTSIRIDGVL HEFTTVPGVK EDVTDIILNL KGLVVSSDDD EPVTMYLRKQ
110 120 130 140 150
GPGVVTAGDI VPPAGVTVHN PDMHIATLND KGKLEVELVV ERGRGYVPAV
160 170 180 190 200
QNKASGAEIG RIPVDSIYSP VLKVTYKVEA TRVEQRTDFD KLIIDVETKN
210 220 230 240 250
SISPRDALAS AGGTLVELFG LARELNADSE HIEIGPSPAE ADHIASFALP
260 270 280 290 300
IDDLDLTVRS YNCLKREGVH TVGELVARTE SDLLDIRNFG QKSIDEVKIK
310 320 330 340 350
LHQLGLSLKD SPATFDPSEV AGYDAATGTW TSDAGYDLDD NQDYAETEQL
Length:350
Mass (Da):37,920
Last modified:January 9, 2007 - v1
Checksum:i87B23F12DFD648E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK71739.1
CP001663 Genomic DNA Translation: AFP37961.1
RefSeqiWP_003892912.1, NZ_CP009494.1
YP_885906.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK71739; ABK71739; MSMEG_1524
AFP37961; AFP37961; MSMEI_1488
GeneIDi4537502
KEGGimsb:LJ00_07615
msg:MSMEI_1488
msm:MSMEG_1524
PATRICifig|246196.19.peg.1509

Similar proteinsi

Entry informationi

Entry nameiRPOA_MYCS2
AccessioniPrimary (citable) accession number: A0QSL8
Secondary accession number(s): I7G472
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: January 9, 2007
Last modified: April 25, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health