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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1524-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:MSMEG_1524, MSMEI_1488
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350DNA-directed RNA polymerase subunit alphaPRO_0000296835Add
BLAST

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation1 Publication

Protein-protein interaction databases

IntActiA0QSL8. 2 interactions.
STRINGi246196.MSMEG_1524.

Structurei

3D structure databases

ProteinModelPortaliA0QSL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 226226Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd
BLAST
Regioni241 – 350110Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd
BLAST

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF. Bacteria.
COG0202. LUCA.
HOGENOMiHOG000218480.
KOiK03040.
OMAiLMKFRNF.
OrthoDBiPOG091H001Z.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QSL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISQRPTLS EETVAENRSR FVIEPLEPGF GYTLGNSLRR TLLSSIPGAA
60 70 80 90 100
VTSIRIDGVL HEFTTVPGVK EDVTDIILNL KGLVVSSDDD EPVTMYLRKQ
110 120 130 140 150
GPGVVTAGDI VPPAGVTVHN PDMHIATLND KGKLEVELVV ERGRGYVPAV
160 170 180 190 200
QNKASGAEIG RIPVDSIYSP VLKVTYKVEA TRVEQRTDFD KLIIDVETKN
210 220 230 240 250
SISPRDALAS AGGTLVELFG LARELNADSE HIEIGPSPAE ADHIASFALP
260 270 280 290 300
IDDLDLTVRS YNCLKREGVH TVGELVARTE SDLLDIRNFG QKSIDEVKIK
310 320 330 340 350
LHQLGLSLKD SPATFDPSEV AGYDAATGTW TSDAGYDLDD NQDYAETEQL
Length:350
Mass (Da):37,920
Last modified:January 9, 2007 - v1
Checksum:i87B23F12DFD648E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71739.1.
CP001663 Genomic DNA. Translation: AFP37961.1.
RefSeqiWP_003892912.1. NZ_CP009494.1.
YP_885906.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71739; ABK71739; MSMEG_1524.
AFP37961; AFP37961; MSMEI_1488.
GeneIDi4537502.
KEGGimsb:LJ00_07615.
msg:MSMEI_1488.
msm:MSMEG_1524.
PATRICi18075505. VBIMycSme59918_1509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71739.1.
CP001663 Genomic DNA. Translation: AFP37961.1.
RefSeqiWP_003892912.1. NZ_CP009494.1.
YP_885906.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QSL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA0QSL8. 2 interactions.
STRINGi246196.MSMEG_1524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71739; ABK71739; MSMEG_1524.
AFP37961; AFP37961; MSMEI_1488.
GeneIDi4537502.
KEGGimsb:LJ00_07615.
msg:MSMEI_1488.
msm:MSMEG_1524.
PATRICi18075505. VBIMycSme59918_1509.

Phylogenomic databases

eggNOGiENOG4105CTF. Bacteria.
COG0202. LUCA.
HOGENOMiHOG000218480.
KOiK03040.
OMAiLMKFRNF.
OrthoDBiPOG091H001Z.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1524-MONOMER.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPOA_MYCS2
AccessioniPrimary (citable) accession number: A0QSL8
Secondary accession number(s): I7G472
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.