A0QSL7 (RS4_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S4

Gene names

Name:	rpsD
Ordered Locus Names:	MSMEG_1523, MSMEI_1487

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit By similarity. HAMAP-Rule MF_01306 With S5 and S12 plays an important role in translational accuracy By similarity. HAMAP-Rule MF_01306
Subunit structure	Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity By similarity.
Sequence similarities	Belongs to the ribosomal protein S4P family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	30S ribosomal protein S4 HAMAP-Rule MF_01306		PRO_0000322311
Regions
	Domain	91 – 157	67	S4 RNA-binding

Sequences

	Sequence		Length	Mass (Da)
	A0QSL7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: F6AC4F66558E0968	FASTA	201	23,376
	10 20 30 40 50 60 MARYTGPATR KSRRLGVDLV GGDQSFEKRP YPPGQHGRAR IKESEYRQQL QEKQKARFSY 70 80 90 100 110 120 GVMEKQFRRY YEEANRQPGK TGDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV 130 140 150 160 170 180 NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER PIPSWLQVVG ERQRILVHQL 190 200 PERAQIDVPL TEQLIVELYS K

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70484.1. CP001663 Genomic DNA. Translation: AFP37960.1.
RefSeq	YP_006566255.1. NC_018289.1. YP_885905.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QSL7.
SMR	A0QSL7. Positions 41-201.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_1523.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70484; ABK70484; MSMEG_1523.
GeneID	13429302. 4533059.
KEGG	msg:MSMEI_1487. msm:MSMEG_1523.
PATRIC	18075503. VBIMycSme59918_1508.
Phylogenomic databases
eggNOG	COG0522.
HOGENOM	HOG000221004.
KO	K02986.
OMA	THGHILV.
ProtClustDB	PRK05327.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-1523-MONOMER.
Family and domain databases
Gene3D	1.10.1050.10. 1 hit. 3.10.290.10. 1 hit.
HAMAP	MF_01306_B. Ribosomal_S4_B.
InterPro	IPR022801. Ribosomal_S4/S9. IPR001912. Ribosomal_S4/S9_N. IPR005709. Ribosomal_S4_bac-type. IPR018079. Ribosomal_S4_CS. IPR002942. S4_RNA-bd. [Graphical view]
PANTHER	PTHR11831. PTHR11831. 1 hit.
Pfam	PF00163. Ribosomal_S4. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR01017. rpsD_bact. 1 hit.
PROSITE	PS00632. RIBOSOMAL_S4. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS4_MYCS2

Accession

Primary (citable) accession number: A0QSL7
Secondary accession number(s): I7F8S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program