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Protein

dTDP-4-dehydrorhamnose 3,5-epimerase

Gene

rmlC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.1 Publication

Catalytic activityi

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose.1 Publication

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateBy similarity
Binding sitei28 – 281SubstrateBy similarity
Binding sitei49 – 491SubstrateBy similarity
Binding sitei59 – 591SubstrateBy similarity
Binding sitei62 – 621SubstrateBy similarity
Binding sitei72 – 721SubstrateBy similarity
Binding sitei83 – 831SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei167 – 1671SubstrateBy similarity

GO - Molecular functioni

  • dTDP-4-dehydrorhamnose 3,5-epimerase activity Source: UniProtKB

GO - Biological processi

  • dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  • extracellular polysaccharide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1511-MONOMER.
UniPathwayiUPA00124.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose 3,5-epimerase (EC:5.1.3.13)
Alternative name(s):
Thymidine diphospho-4-keto-rhamnose 3,5-epimerase
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase
dTDP-L-rhamnose synthase
Gene namesi
Name:rmlC
Ordered Locus Names:MSMEG_1510, MSMEI_1475
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201dTDP-4-dehydrorhamnose 3,5-epimerasePRO_0000399899Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_1510.

Structurei

3D structure databases

ProteinModelPortaliA0QSK5.
SMRiA0QSK5. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108URP. Bacteria.
COG1898. LUCA.
HOGENOMiHOG000227724.
KOiK01790.
OMAiEPPILSE.
OrthoDBiPOG091H04OS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiPF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]
ProDomiPD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QSK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARELSIAG AWEITPVLRT DSRGLFFEWF TDAGFTEFAG HQFDMRQANC
60 70 80 90 100
SVSARGVLRG VHFAQVPPSQ AKYVTCVRGA VFDVVVDIRV GSPTFGQWDA
110 120 130 140 150
VLLDDKDRRS IYISEGLGHA FLALDDDSTV MYLCSAPYAP QREHTVRPTD
160 170 180 190 200
FGIEWPEVPE LILSDRDAQA PSLAEAQAAG VLPTWADCQA FVETLRRNLV

S
Length:201
Mass (Da):22,241
Last modified:January 9, 2007 - v1
Checksum:i7E3E273F547CBE7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71239.1.
CP001663 Genomic DNA. Translation: AFP37948.1.
RefSeqiWP_011727714.1. NZ_CP009494.1.
YP_885893.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71239; ABK71239; MSMEG_1510.
AFP37948; AFP37948; MSMEI_1475.
GeneIDi4537242.
KEGGimsb:LJ00_07550.
msg:MSMEI_1475.
msm:MSMEG_1510.
PATRICi18075479. VBIMycSme59918_1496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71239.1.
CP001663 Genomic DNA. Translation: AFP37948.1.
RefSeqiWP_011727714.1. NZ_CP009494.1.
YP_885893.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QSK5.
SMRiA0QSK5. Positions 1-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71239; ABK71239; MSMEG_1510.
AFP37948; AFP37948; MSMEI_1475.
GeneIDi4537242.
KEGGimsb:LJ00_07550.
msg:MSMEI_1475.
msm:MSMEG_1510.
PATRICi18075479. VBIMycSme59918_1496.

Phylogenomic databases

eggNOGiENOG4108URP. Bacteria.
COG1898. LUCA.
HOGENOMiHOG000227724.
KOiK01790.
OMAiEPPILSE.
OrthoDBiPOG091H04OS.

Enzyme and pathway databases

UniPathwayiUPA00124.
BioCyciMSME246196:GJ4Y-1511-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiPF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]
ProDomiPD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRMLC_MYCS2
AccessioniPrimary (citable) accession number: A0QSK5
Secondary accession number(s): I7FGH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.