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Protein

1,4-dihydroxy-2-naphthoyl-CoA synthase

Gene

menB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).UniRule annotation1 Publication

Catalytic activityi

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H2O.UniRule annotation1 Publication

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. no protein annotated in this organism
  4. o-succinylbenzoate synthase (menC), o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateUniRule annotation
Binding sitei110 – 1101SubstrateUniRule annotation
Sitei110 – 1101Important for catalysisUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotationBy similarity
Sitei180 – 1801Important for catalysisUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotationBy similarity
Binding sitei282 – 2821Substrate; shared with neighboring subunitUniRule annotation
Sitei282 – 2821Important for catalysisUniRule annotation
Binding sitei297 – 2971Substrate; shared with neighboring subunitUniRule annotation

GO - Molecular functioni

  • 1,4-dihydroxy-2-naphthoyl-CoA synthase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-1075-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00167.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-dihydroxy-2-naphthoyl-CoA synthaseUniRule annotation (EC:4.1.3.36UniRule annotation)
Short name:
DHNA-CoA synthaseUniRule annotation
Gene namesi
Name:menBUniRule annotation
Ordered Locus Names:MSMEG_1075, MSMEI_1042
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3093091,4-dihydroxy-2-naphthoyl-CoA synthasePRO_0000403187Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_1075.

Structurei

3D structure databases

ProteinModelPortaliA0QRD3.
SMRiA0QRD3. Positions 9-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1025Substrate bindingUniRule annotation
Regioni152 – 1565Substrate bindingUniRule annotationBy similarity

Sequence similaritiesi

Belongs to the enoyl-CoA hydratase/isomerase family. MenB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SIQ. Bacteria.
COG0447. LUCA.
HOGENOMiHOG000027942.
KOiK01661.
OMAiKYAFCSG.
OrthoDBiPOG091H05HP.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01934. MenB. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR010198. DHNA-CoA_synthase_MenB.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR01929. menB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QRD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQAASDNP FDPAMWERVP GFDDLTDITY HRHVLDGARQ PTVRVAFDRP
60 70 80 90 100
EVRNAFRPHT VDELYRVLDH ARMSSDVGVI LLTGNGPSPK DGGWAFCSGG
110 120 130 140 150
DQRIRGRTGY QYASGETAET VDPARAGRLH ILEVQRLIRF MPKVVICLVN
160 170 180 190 200
GWAAGGGHSL HVTCDLTLAS REHARFKQTD ADVGSFDGGF GSAYLARQTG
210 220 230 240 250
QKFAREIFFL GRAYDAQTMH QMGAVNEVVD HADLEKAGLQ YAAEINGKSP
260 270 280 290 300
QAIRMLKFAF NLIDDGLVGQ QVFAGEATRL AYMTDEAVEG RDAFLEKRDP

DWSRFPRYF
Length:309
Mass (Da):34,303
Last modified:January 9, 2007 - v1
Checksum:iCACC8113C74964B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74961.1.
CP001663 Genomic DNA. Translation: AFP37522.1.
RefSeqiWP_003892442.1. NZ_CP009494.1.
YP_885471.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74961; ABK74961; MSMEG_1075.
AFP37522; AFP37522; MSMEI_1042.
GeneIDi4531869.
KEGGimsb:LJ00_05340.
msg:MSMEI_1042.
msm:MSMEG_1075.
PATRICi18074602. VBIMycSme59918_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74961.1.
CP001663 Genomic DNA. Translation: AFP37522.1.
RefSeqiWP_003892442.1. NZ_CP009494.1.
YP_885471.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QRD3.
SMRiA0QRD3. Positions 9-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1075.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74961; ABK74961; MSMEG_1075.
AFP37522; AFP37522; MSMEI_1042.
GeneIDi4531869.
KEGGimsb:LJ00_05340.
msg:MSMEI_1042.
msm:MSMEG_1075.
PATRICi18074602. VBIMycSme59918_1062.

Phylogenomic databases

eggNOGiENOG4107SIQ. Bacteria.
COG0447. LUCA.
HOGENOMiHOG000027942.
KOiK01661.
OMAiKYAFCSG.
OrthoDBiPOG091H05HP.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00167.
BioCyciMSME246196:GJ4Y-1075-MONOMER.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01934. MenB. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR010198. DHNA-CoA_synthase_MenB.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR01929. menB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENB_MYCS2
AccessioniPrimary (citable) accession number: A0QRD3
Secondary accession number(s): I7FXI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.