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A0QR54

- MTAP_MYCS2

UniProt

A0QR54 - MTAP_MYCS2

Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Kineticsi

    1. KM=1.6 µM for S-methyl-5'-thioadenosine1 Publication
    2. KM=310 µM for adenosine1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91PhosphateUniRule annotation
    Sitei157 – 1571Important for substrate specificityUniRule annotation
    Binding sitei175 – 1751Substrate; via amide nitrogenUniRule annotation
    Binding sitei176 – 1761PhosphateUniRule annotation
    Sitei212 – 2121Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-990-MONOMER.
    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Gene namesi
    Name:mtnPUniRule annotation
    Synonyms:pnp
    Ordered Locus Names:MSMEG_0990, MSMEI_0963
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259S-methyl-5'-thioadenosine phosphorylasePRO_0000415095Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0990.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QR54.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 512Phosphate bindingUniRule annotation
    Regioni199 – 2013Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiHEFSAHT.
    OrthoDBiEOG6KHFXC.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0QR54-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMLGVIGGSG FYTFFGSDAR AVSVETPYGP PSAPITVGTV GDHEVAFLPR    50
    HGVKHEFSPH TVPYRANLWA LRSLGVRRVF APCAVGSLTP DLGPGSIVVP 100
    DQLVDRTSGR DDTYFDSGGI HVAFADPYCP TLRAAATGLP GVVDGGTMVV 150
    IQGPRFSTRA ESRWFASQGF TLVNMTGYPE AVLARELEMC YAAVALVTDL 200
    DAGIEVGSGV RAVDVFAEFE RNMPPFKKLV FEALEAVEVE RTCTHCLTHS 250
    GVQLPFELP 259
    Length:259
    Mass (Da):27,723
    Last modified:January 9, 2007 - v1
    Checksum:i8D379609536F84FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70512.1.
    CP001663 Genomic DNA. Translation: AFP37443.1.
    RefSeqiYP_006565738.1. NC_018289.1.
    YP_885392.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70512; ABK70512; MSMEG_0990.
    AFP37443; AFP37443; MSMEI_0963.
    GeneIDi4532080.
    KEGGimsg:MSMEI_0963.
    msm:MSMEG_0990.
    PATRICi18074434. VBIMycSme59918_0978.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK70512.1 .
    CP001663 Genomic DNA. Translation: AFP37443.1 .
    RefSeqi YP_006565738.1. NC_018289.1.
    YP_885392.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QR54.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_0990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70512 ; ABK70512 ; MSMEG_0990 .
    AFP37443 ; AFP37443 ; MSMEI_0963 .
    GeneIDi 4532080.
    KEGGi msg:MSMEI_0963.
    msm:MSMEG_0990.
    PATRICi 18074434. VBIMycSme59918_0978.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi HEFSAHT.
    OrthoDBi EOG6KHFXC.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .
    BioCyci MSME246196:GJ4Y-990-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Identification and characterization of two adenosine phosphorylase activities in Mycobacterium smegmatis."
      Buckoreelall K., Wilson L., Parker W.B.
      J. Bacteriol. 193:5668-5674(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiMTAP_MYCS2
    AccessioniPrimary (citable) accession number: A0QR54
    Secondary accession number(s): I7FF12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3