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A0QR54

- MTAP_MYCS2

UniProt

A0QR54 - MTAP_MYCS2

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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Kineticsi

  1. KM=1.6 µM for S-methyl-5'-thioadenosine1 Publication
  2. KM=310 µM for adenosine1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91PhosphateUniRule annotation
Sitei157 – 1571Important for substrate specificityUniRule annotation
Binding sitei175 – 1751Substrate; via amide nitrogenUniRule annotation
Binding sitei176 – 1761PhosphateUniRule annotation
Sitei212 – 2121Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-990-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Synonyms:pnp
Ordered Locus Names:MSMEG_0990, MSMEI_0963
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259S-methyl-5'-thioadenosine phosphorylasePRO_0000415095Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_0990.

Structurei

3D structure databases

ProteinModelPortaliA0QR54.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Phosphate bindingUniRule annotation
Regioni199 – 2013Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
KOiK00772.
OMAiHEFSAHT.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QR54-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMLGVIGGSG FYTFFGSDAR AVSVETPYGP PSAPITVGTV GDHEVAFLPR
60 70 80 90 100
HGVKHEFSPH TVPYRANLWA LRSLGVRRVF APCAVGSLTP DLGPGSIVVP
110 120 130 140 150
DQLVDRTSGR DDTYFDSGGI HVAFADPYCP TLRAAATGLP GVVDGGTMVV
160 170 180 190 200
IQGPRFSTRA ESRWFASQGF TLVNMTGYPE AVLARELEMC YAAVALVTDL
210 220 230 240 250
DAGIEVGSGV RAVDVFAEFE RNMPPFKKLV FEALEAVEVE RTCTHCLTHS

GVQLPFELP
Length:259
Mass (Da):27,723
Last modified:January 9, 2007 - v1
Checksum:i8D379609536F84FF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70512.1.
CP001663 Genomic DNA. Translation: AFP37443.1.
RefSeqiWP_011727338.1. NC_018289.1.
YP_006565738.1. NC_018289.1.
YP_885392.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70512; ABK70512; MSMEG_0990.
AFP37443; AFP37443; MSMEI_0963.
GeneIDi4532080.
KEGGimsg:MSMEI_0963.
msm:MSMEG_0990.
PATRICi18074434. VBIMycSme59918_0978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK70512.1 .
CP001663 Genomic DNA. Translation: AFP37443.1 .
RefSeqi WP_011727338.1. NC_018289.1.
YP_006565738.1. NC_018289.1.
YP_885392.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0QR54.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_0990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK70512 ; ABK70512 ; MSMEG_0990 .
AFP37443 ; AFP37443 ; MSMEI_0963 .
GeneIDi 4532080.
KEGGi msg:MSMEI_0963.
msm:MSMEG_0990.
PATRICi 18074434. VBIMycSme59918_0978.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228987.
KOi K00772.
OMAi HEFSAHT.
OrthoDBi EOG6KHFXC.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00873 .
BioCyci MSME246196:GJ4Y-990-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
TIGRFAMsi TIGR01694. MTAP. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Identification and characterization of two adenosine phosphorylase activities in Mycobacterium smegmatis."
    Buckoreelall K., Wilson L., Parker W.B.
    J. Bacteriol. 193:5668-5674(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiMTAP_MYCS2
AccessioniPrimary (citable) accession number: A0QR54
Secondary accession number(s): I7FF12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3