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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.17 mM for UDP-GlcNAc1 Publication
  2. KM=0.15 mM for 1D-inositol 3-phosphate1 Publication
  1. Vmax=0.7 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 1911D-inositol 3-phosphateBy similarity
Binding sitei33 – 331UDP-GlcNAc; via amide nitrogenBy similarity
Binding sitei88 – 8811D-inositol 3-phosphateBy similarity
Binding sitei121 – 12111D-inositol 3-phosphateBy similarity
Binding sitei145 – 14511D-inositol 3-phosphateBy similarity
Binding sitei165 – 16511D-inositol 3-phosphateBy similarity
Binding sitei239 – 2391UDP-GlcNAcBy similarity
Binding sitei244 – 2441UDP-GlcNAcBy similarity
Binding sitei297 – 2971UDP-GlcNAc; via amide nitrogen and carbonyl oxygen
Metal bindingi306 – 3061Magnesium; via carbonyl oxygenBy similarity
Metal bindingi307 – 3071Magnesium; via carbonyl oxygenBy similarity
Metal bindingi309 – 3091Magnesium; via carbonyl oxygenBy similarity
Binding sitei319 – 3191UDP-GlcNAcBy similarity
Binding sitei327 – 3271UDP-GlcNAcBy similarity
Metal bindingi333 – 3331MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-933-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferase
Gene namesi
Name:mshA
Ordered Locus Names:MSMEG_0933, MSMEI_0910
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434D-inositol 3-phosphate glycosyltransferasePRO_0000399824Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_0933.

Structurei

3D structure databases

ProteinModelPortaliA0QQZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 262UDP-GlcNAc bindingBy similarity
Regioni30 – 3561D-inositol 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QQZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLATDLETP RRVAVLSVHT SPLAQPGTGD AGGMNVYVLQ TALQLARRGV
60 70 80 90 100
EVEVFTRATS SADAPVVPVA PGVLVRNVVA GPFEGLDKND LPTQLCAFTA
110 120 130 140 150
GVLRAEATHE PGYYDVVHSH YWLSGQVGWL ARDRWAVPLV HTAHTLAAVK
160 170 180 190 200
NAALAAGDAP EPPLRAVGEQ QVVDEADRLI VNTEVEAQQL VSLHNADRSR
210 220 230 240 250
IDVVHPGVDL DVFTPGSRDA ARAVFGLPTD QKIVAFVGRI QPLKAPDILL
260 270 280 290 300
RAAAKLPGVR VLIAGGPSGS GLAQPDTLVR LADELGISDR VTFLPPQSRE
310 320 330 340 350
QLVNVYRAAD LVAVPSYSES FGLVAVEAQA CGTPVVAAAV GGLPVAVADG
360 370 380 390 400
VSGALVDGHD IGDWADTISE VLDREPAALS RASAEHAAQF SWAHTVDALL
410 420 430
ASYSRAMSDY RARHPRPAAR RSGRRFSMRR GVRT
Length:434
Mass (Da):45,935
Last modified:January 9, 2007 - v1
Checksum:i2F321E8580DE4B83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK76112.1.
CP001663 Genomic DNA. Translation: AFP37390.1.
RefSeqiWP_011727296.1. NZ_CP009494.1.
YP_885336.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK76112; ABK76112; MSMEG_0933.
AFP37390; AFP37390; MSMEI_0910.
GeneIDi4537367.
KEGGimsb:LJ00_04630.
msg:MSMEI_0910.
msm:MSMEG_0933.
PATRICi18074324. VBIMycSme59918_0923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK76112.1.
CP001663 Genomic DNA. Translation: AFP37390.1.
RefSeqiWP_011727296.1. NZ_CP009494.1.
YP_885336.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QQZ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_0933.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK76112; ABK76112; MSMEG_0933.
AFP37390; AFP37390; MSMEI_0910.
GeneIDi4537367.
KEGGimsb:LJ00_04630.
msg:MSMEI_0910.
msm:MSMEG_0933.
PATRICi18074324. VBIMycSme59918_0923.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-933-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCS2
AccessioniPrimary (citable) accession number: A0QQZ8
Secondary accession number(s): I7F752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.