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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.17 mM for UDP-GlcNAc1 Publication
  2. KM=0.15 mM for 1D-inositol 3-phosphate1 Publication
  1. Vmax=0.7 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191D-inositol 3-phosphateBy similarity1
Binding sitei33UDP-GlcNAc; via amide nitrogenBy similarity1
Binding sitei881D-inositol 3-phosphateBy similarity1
Binding sitei1211D-inositol 3-phosphateBy similarity1
Binding sitei1451D-inositol 3-phosphateBy similarity1
Binding sitei1651D-inositol 3-phosphateBy similarity1
Binding sitei239UDP-GlcNAcBy similarity1
Binding sitei244UDP-GlcNAcBy similarity1
Binding sitei297UDP-GlcNAc; via amide nitrogen and carbonyl oxygen1
Metal bindingi306Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi307Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi309Magnesium; via carbonyl oxygenBy similarity1
Binding sitei319UDP-GlcNAcBy similarity1
Binding sitei327UDP-GlcNAcBy similarity1
Metal bindingi333MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-937-MONOMER

Protein family/group databases

CAZyiGT4 Glycosyltransferase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferase
Gene namesi
Name:mshA
Ordered Locus Names:MSMEG_0933, MSMEI_0910
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003998241 – 434D-inositol 3-phosphate glycosyltransferaseAdd BLAST434

Proteomic databases

PRIDEiA0QQZ8

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_0933

Structurei

3D structure databases

ProteinModelPortaliA0QQZ8
SMRiA0QQZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 26UDP-GlcNAc bindingBy similarity2
Regioni30 – 351D-inositol 3-phosphate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R5M Bacteria
COG0438 LUCA
HOGENOMiHOG000077288
KOiK15521
OMAiHTMAKVK
OrthoDBiPOG091H0AO3

Family and domain databases

HAMAPiMF_01695 MshA, 1 hit
InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR028098 Glyco_trans_4-like_N
IPR017814 Mycothiol_biosynthesis_MshA
PfamiView protein in Pfam
PF13439 Glyco_transf_4, 1 hit
PF00534 Glycos_transf_1, 1 hit
TIGRFAMsiTIGR03449 mycothiol_MshA, 1 hit

Sequencei

Sequence statusi: Complete.

A0QQZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLATDLETP RRVAVLSVHT SPLAQPGTGD AGGMNVYVLQ TALQLARRGV
60 70 80 90 100
EVEVFTRATS SADAPVVPVA PGVLVRNVVA GPFEGLDKND LPTQLCAFTA
110 120 130 140 150
GVLRAEATHE PGYYDVVHSH YWLSGQVGWL ARDRWAVPLV HTAHTLAAVK
160 170 180 190 200
NAALAAGDAP EPPLRAVGEQ QVVDEADRLI VNTEVEAQQL VSLHNADRSR
210 220 230 240 250
IDVVHPGVDL DVFTPGSRDA ARAVFGLPTD QKIVAFVGRI QPLKAPDILL
260 270 280 290 300
RAAAKLPGVR VLIAGGPSGS GLAQPDTLVR LADELGISDR VTFLPPQSRE
310 320 330 340 350
QLVNVYRAAD LVAVPSYSES FGLVAVEAQA CGTPVVAAAV GGLPVAVADG
360 370 380 390 400
VSGALVDGHD IGDWADTISE VLDREPAALS RASAEHAAQF SWAHTVDALL
410 420 430
ASYSRAMSDY RARHPRPAAR RSGRRFSMRR GVRT
Length:434
Mass (Da):45,935
Last modified:January 9, 2007 - v1
Checksum:i2F321E8580DE4B83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK76112.1
CP001663 Genomic DNA Translation: AFP37390.1
RefSeqiWP_011727296.1, NZ_CP009494.1
YP_885336.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK76112; ABK76112; MSMEG_0933
AFP37390; AFP37390; MSMEI_0910
GeneIDi4537367
KEGGimsb:LJ00_04630
msg:MSMEI_0910
msm:MSMEG_0933
PATRICifig|246196.19.peg.923

Similar proteinsi

Entry informationi

Entry nameiMSHA_MYCS2
AccessioniPrimary (citable) accession number: A0QQZ8
Secondary accession number(s): I7F752
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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