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Protein

Thiazole synthase

Gene

thiG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Schiff-base intermediate with DXPUniRule annotation1
Binding sitei159DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-801-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:MSMEG_0793, MSMEI_0777
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_10000260132 – 252Thiazole synthaseAdd BLAST251

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_0793

Structurei

3D structure databases

ProteinModelPortaliA0QQL0
SMRiA0QQL0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186DXP bindingUniRule annotation2
Regioni207 – 208DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QQL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSVLRIGG REFGSRLIMG TGGAPNLSVL EEALIASGTE LTTVAMRRVD
60 70 80 90 100
AETGTGVLDL LNRLGIAALP NTAGCRGAAE AVLTAQLARE ALGTDMVKLE
110 120 130 140 150
VIADERTLLP DAVELVKAAE QLVDDGFTVL PYTNDDPVLA RRLEDIGCAA
160 170 180 190 200
VMPLGSPIGT GLGISNPHNI EMIVAAAGVP VVLDAGIGTA SDAALAMELG
210 220 230 240 250
CDAVLLATAV TRASDPPTMA AAMASAVTAG HLARQAGRIP KRFWAQASSP

AL
Length:252
Mass (Da):25,645
Last modified:January 9, 2007 - v1
Checksum:i3C4308CCC2B1F1A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK71483.1
CP001663 Genomic DNA Translation: AFP37257.1
RefSeqiWP_011727197.1, NZ_CP009494.1
YP_885198.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK71483; ABK71483; MSMEG_0793
AFP37257; AFP37257; MSMEI_0777
GeneIDi4532639
KEGGimsb:LJ00_03940
msg:MSMEI_0777
msm:MSMEG_0793
PATRICifig|246196.19.peg.788

Similar proteinsi

Entry informationi

Entry nameiTHIG_MYCS2
AccessioniPrimary (citable) accession number: A0QQL0
Secondary accession number(s): I7FX04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: June 20, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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