A0QQK3 (PKNG_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknG
EC=2.7.11.1

Gene names

Name:	pknG
Ordered Locus Names:	MSMEG_0786, MSMEI_0770

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.5
Subunit structure	Interacts with GarA in vitro. Ref.4
Miscellaneous	When artificially expressed from expression vectors, encodes an active kinase, which is able to block the lysosomal delivery of mycobacteria inside macrophages. However, while PknG transcripts are found in M.smegmatis, expression of PknG is blocked on a translational level in this non-pathogenic species. This lack of efficient translation is caused by regulatory elements in the upstream region of the gene (Ref.5).
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Serine/threonine-protein kinase PknG		PRO_0000419491
Regions
	Domain	161 – 403	243	Protein kinase
	Nucleotide binding	167 – 175	9	ATP By similarity
Sites
	Active site	286	1	Proton acceptor By similarity
	Binding site	191	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QQK3 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 417893787A19511B	FASTA	760	82,645
	10 20 30 40 50 60 MTSPENPDLP DADDAYVDSG PGTQPASLED LDMDSASTMR PMATQAVYRP EFDDTDGTSR 70 80 90 100 110 120 GTVVTEAYDQ VTMATRALSP MRRLGGGLVE IPRVPERDPL TALMTNPVVA ESKRFCWNCG 130 140 150 160 170 180 KPVGRSTPDG RALSEGWCPH CGSPYSFLPQ LSPGDIVADQ YEIKGCIAHG GLGWVYLAFD 190 200 210 220 230 240 KNVNDRPVVL KGLVHSGDAE AQAIAMAERQ FLAEVTHPGI VKIYNFVEHE DKHGNPVGYI 250 260 270 280 290 300 VMEYVGGTSL KQARGAKLPV AEAIGYMLEI LPALGYLHSI GLAYNDLKPE NIMITEEQLK 310 320 330 340 350 360 LIDLGAVSRL NSYGYLYGTP GYQAPEIVRT GPTVATDIYT VGRTLAALTL SLRTRRGRYV 370 380 390 400 410 420 DGLPSDDPVL ETYDSYHRLL RRAIDPDPRR RFTSAEEMSS QLLGVLREVV ATDTGVPRPG 430 440 450 460 470 480 LSTVFSPSRS TFGVDLLVAH TDVYVDGQVH SEKLTAQEIV RALPVPLVDR TDVGAPMLVA 490 500 510 520 530 540 SVLSEPVHTL DQLRAARHGA LDTEGIDLNE SVELPLMEVR ALLDLGDVAK ATRKLEDLAA 550 560 570 580 590 600 RVGWRWRLVW FKAVSEMLSA DYDSATKHFT EVLDTLPGEL APKLALAATA ELAGTADELK 610 620 630 640 650 660 FYKTVWSTDN GVISAGFGLA RAQSVAGERD MAVQTLDEVP PTSRHFTTAR LTSAVTLLSG 670 680 690 700 710 720 RSTSEITEQH IRDAARRVEA LPDSEPRVLQ IRALVLGTAL DWLADNTASS NHILGFPFTE 730 740 750 760 HGLKLGVEAS LRALARIAPT QSHRYALVDL ANSVRPMSTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Regulation of glutamate metabolism by protein kinases in mycobacteria." O'Hare H.M., Duran R., Cervenansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M. Mol. Microbiol. 70:1408-1423(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GARA. Strain: ATCC 700084 / mc(2)155.
[5]	"Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria." Houben E.N., Walburger A., Ferrari G., Nguyen L., Thompson C.J., Miess C., Vogel G., Mueller B., Pieters J. Mol. Microbiol. 72:41-52(2009) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, LACK OF TRANSLATION IN VIVO. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71095.1. CP001663 Genomic DNA. Translation: AFP37250.1.
RefSeq	YP_006565545.1. NC_018289.1. YP_885191.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QQK3.
SMR	A0QQK3. Positions 84-756.
ModBase	Search...
Protein-protein interaction databases
IntAct	A0QQK3. 1 interaction.
STRING	246196.MSMEG_0786.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK71095; ABK71095; MSMEG_0786. AFP37250; AFP37250; MSMEI_0770.
GeneID	13427940. 4537074.
KEGG	msg:MSMEI_0770. msm:MSMEG_0786.
PATRIC	18074040. VBIMycSme59918_0781.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000049016.
KO	K14949.
OMA	HRYALVD.
ProtClustDB	CLSK790502.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-786-MONOMER.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNG_MYCS2

Accession

Primary (citable) accession number: A0QQK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2012
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program