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A0QQK3 (PKNG_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PknG

EC=2.7.11.1
Gene names
Name:pknG
Ordered Locus Names:MSMEG_0786, MSMEI_0770
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length760 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.5

Subunit structure

Interacts with GarA in vitro. Ref.4

Miscellaneous

When artificially expressed from expression vectors, encodes an active kinase, which is able to block the lysosomal delivery of mycobacteria inside macrophages. However, while PknG transcripts are found in M.smegmatis, expression of PknG is blocked on a translational level in this non-pathogenic species. This lack of efficient translation is caused by regulatory elements in the upstream region of the gene (Ref.5).

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 760760Serine/threonine-protein kinase PknG
PRO_0000419491

Regions

Domain161 – 403243Protein kinase
Nucleotide binding167 – 1759ATP By similarity

Sites

Active site2861Proton acceptor By similarity
Binding site1911ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0QQK3 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 417893787A19511B

FASTA76082,645
        10         20         30         40         50         60 
MTSPENPDLP DADDAYVDSG PGTQPASLED LDMDSASTMR PMATQAVYRP EFDDTDGTSR 

        70         80         90        100        110        120 
GTVVTEAYDQ VTMATRALSP MRRLGGGLVE IPRVPERDPL TALMTNPVVA ESKRFCWNCG 

       130        140        150        160        170        180 
KPVGRSTPDG RALSEGWCPH CGSPYSFLPQ LSPGDIVADQ YEIKGCIAHG GLGWVYLAFD 

       190        200        210        220        230        240 
KNVNDRPVVL KGLVHSGDAE AQAIAMAERQ FLAEVTHPGI VKIYNFVEHE DKHGNPVGYI 

       250        260        270        280        290        300 
VMEYVGGTSL KQARGAKLPV AEAIGYMLEI LPALGYLHSI GLAYNDLKPE NIMITEEQLK 

       310        320        330        340        350        360 
LIDLGAVSRL NSYGYLYGTP GYQAPEIVRT GPTVATDIYT VGRTLAALTL SLRTRRGRYV 

       370        380        390        400        410        420 
DGLPSDDPVL ETYDSYHRLL RRAIDPDPRR RFTSAEEMSS QLLGVLREVV ATDTGVPRPG 

       430        440        450        460        470        480 
LSTVFSPSRS TFGVDLLVAH TDVYVDGQVH SEKLTAQEIV RALPVPLVDR TDVGAPMLVA 

       490        500        510        520        530        540 
SVLSEPVHTL DQLRAARHGA LDTEGIDLNE SVELPLMEVR ALLDLGDVAK ATRKLEDLAA 

       550        560        570        580        590        600 
RVGWRWRLVW FKAVSEMLSA DYDSATKHFT EVLDTLPGEL APKLALAATA ELAGTADELK 

       610        620        630        640        650        660 
FYKTVWSTDN GVISAGFGLA RAQSVAGERD MAVQTLDEVP PTSRHFTTAR LTSAVTLLSG 

       670        680        690        700        710        720 
RSTSEITEQH IRDAARRVEA LPDSEPRVLQ IRALVLGTAL DWLADNTASS NHILGFPFTE 

       730        740        750        760 
HGLKLGVEAS LRALARIAPT QSHRYALVDL ANSVRPMSTF 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Regulation of glutamate metabolism by protein kinases in mycobacteria."
O'Hare H.M., Duran R., Cervenansky C., Bellinzoni M., Wehenkel A.M., Pritsch O., Obal G., Baumgartner J., Vialaret J., Johnsson K., Alzari P.M.
Mol. Microbiol. 70:1408-1423(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GARA.
Strain: ATCC 700084 / mc(2)155.
[5]"Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria."
Houben E.N., Walburger A., Ferrari G., Nguyen L., Thompson C.J., Miess C., Vogel G., Mueller B., Pieters J.
Mol. Microbiol. 72:41-52(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, LACK OF TRANSLATION IN VIVO.
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK71095.1.
CP001663 Genomic DNA. Translation: AFP37250.1.
RefSeqYP_006565545.1. NC_018289.1.
YP_885191.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0QQK3.
SMRA0QQK3. Positions 84-756.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActA0QQK3. 1 interaction.
STRING246196.MSMEG_0786.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK71095; ABK71095; MSMEG_0786.
AFP37250; AFP37250; MSMEI_0770.
GeneID13427940.
4537074.
KEGGmsg:MSMEI_0770.
msm:MSMEG_0786.
PATRIC18074040. VBIMycSme59918_0781.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000049016.
KOK14949.
OMAHRYALVD.
OrthoDBEOG6B35XT.
ProtClustDBCLSK790502.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-786-MONOMER.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKNG_MYCS2
AccessionPrimary (citable) accession number: A0QQK3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: January 9, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families