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Protein

Serine/threonine-protein kinase PknG

Gene

pknG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191ATPPROSITE-ProRule annotation1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 175ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknG (EC:2.7.11.1)
Gene namesi
Name:pknG
Ordered Locus Names:MSMEG_0786, MSMEI_0770
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004194911 – 760Serine/threonine-protein kinase PknGAdd BLAST760

Interactioni

Subunit structurei

Interacts with GarA in vitro.1 Publication

Protein-protein interaction databases

IntActiA0QQK3. 1 interactor.
STRINGi246196.MSMEG_0786.

Structurei

3D structure databases

ProteinModelPortaliA0QQK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini161 – 403Protein kinasePROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107Q25. Bacteria.
COG0515. LUCA.
HOGENOMiHOG000049016.
KOiK14949.
OMAiFPFTEHG.
OrthoDBiPOG091H0264.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR031634. PknG_rubred.
IPR031636. PknG_TPR.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF16919. PknG_rubred. 1 hit.
PF16918. PknG_TPR. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QQK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPENPDLP DADDAYVDSG PGTQPASLED LDMDSASTMR PMATQAVYRP
60 70 80 90 100
EFDDTDGTSR GTVVTEAYDQ VTMATRALSP MRRLGGGLVE IPRVPERDPL
110 120 130 140 150
TALMTNPVVA ESKRFCWNCG KPVGRSTPDG RALSEGWCPH CGSPYSFLPQ
160 170 180 190 200
LSPGDIVADQ YEIKGCIAHG GLGWVYLAFD KNVNDRPVVL KGLVHSGDAE
210 220 230 240 250
AQAIAMAERQ FLAEVTHPGI VKIYNFVEHE DKHGNPVGYI VMEYVGGTSL
260 270 280 290 300
KQARGAKLPV AEAIGYMLEI LPALGYLHSI GLAYNDLKPE NIMITEEQLK
310 320 330 340 350
LIDLGAVSRL NSYGYLYGTP GYQAPEIVRT GPTVATDIYT VGRTLAALTL
360 370 380 390 400
SLRTRRGRYV DGLPSDDPVL ETYDSYHRLL RRAIDPDPRR RFTSAEEMSS
410 420 430 440 450
QLLGVLREVV ATDTGVPRPG LSTVFSPSRS TFGVDLLVAH TDVYVDGQVH
460 470 480 490 500
SEKLTAQEIV RALPVPLVDR TDVGAPMLVA SVLSEPVHTL DQLRAARHGA
510 520 530 540 550
LDTEGIDLNE SVELPLMEVR ALLDLGDVAK ATRKLEDLAA RVGWRWRLVW
560 570 580 590 600
FKAVSEMLSA DYDSATKHFT EVLDTLPGEL APKLALAATA ELAGTADELK
610 620 630 640 650
FYKTVWSTDN GVISAGFGLA RAQSVAGERD MAVQTLDEVP PTSRHFTTAR
660 670 680 690 700
LTSAVTLLSG RSTSEITEQH IRDAARRVEA LPDSEPRVLQ IRALVLGTAL
710 720 730 740 750
DWLADNTASS NHILGFPFTE HGLKLGVEAS LRALARIAPT QSHRYALVDL
760
ANSVRPMSTF
Length:760
Mass (Da):82,645
Last modified:January 9, 2007 - v1
Checksum:i417893787A19511B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71095.1.
CP001663 Genomic DNA. Translation: AFP37250.1.
RefSeqiWP_011727190.1. NZ_CP009494.1.
YP_885191.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71095; ABK71095; MSMEG_0786.
AFP37250; AFP37250; MSMEI_0770.
GeneIDi4537074.
KEGGimsb:LJ00_03905.
msg:MSMEI_0770.
msm:MSMEG_0786.
PATRICi18074040. VBIMycSme59918_0781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71095.1.
CP001663 Genomic DNA. Translation: AFP37250.1.
RefSeqiWP_011727190.1. NZ_CP009494.1.
YP_885191.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QQK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA0QQK3. 1 interactor.
STRINGi246196.MSMEG_0786.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71095; ABK71095; MSMEG_0786.
AFP37250; AFP37250; MSMEI_0770.
GeneIDi4537074.
KEGGimsb:LJ00_03905.
msg:MSMEI_0770.
msm:MSMEG_0786.
PATRICi18074040. VBIMycSme59918_0781.

Phylogenomic databases

eggNOGiENOG4107Q25. Bacteria.
COG0515. LUCA.
HOGENOMiHOG000049016.
KOiK14949.
OMAiFPFTEHG.
OrthoDBiPOG091H0264.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR031634. PknG_rubred.
IPR031636. PknG_TPR.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF16919. PknG_rubred. 1 hit.
PF16918. PknG_TPR. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKNG_MYCS2
AccessioniPrimary (citable) accession number: A0QQK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

When artificially expressed from expression vectors, encodes an active kinase, which is able to block the lysosomal delivery of mycobacteria inside macrophages. However, while PknG transcripts are found in M.smegmatis, expression of PknG is blocked on a translational level in this non-pathogenic species. This lack of efficient translation is caused by regulatory elements in the upstream region of the gene (PubMed:19210624).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.