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A0QQK3

- PKNG_MYCS2

UniProt

A0QQK3 - PKNG_MYCS2

Protein

Serine/threonine-protein kinase PknG

Gene

pknG

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei191 – 1911ATPPROSITE-ProRule annotation
    Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi167 – 1759ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-786-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase PknG (EC:2.7.11.1)
    Gene namesi
    Name:pknG
    Ordered Locus Names:MSMEG_0786, MSMEI_0770
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 760760Serine/threonine-protein kinase PknGPRO_0000419491Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with GarA in vitro.1 Publication

    Protein-protein interaction databases

    IntActiA0QQK3. 1 interaction.
    STRINGi246196.MSMEG_0786.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QQK3.
    SMRiA0QQK3. Positions 84-756.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini161 – 403243Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000049016.
    KOiK14949.
    OMAiHRYALVD.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QQK3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSPENPDLP DADDAYVDSG PGTQPASLED LDMDSASTMR PMATQAVYRP    50
    EFDDTDGTSR GTVVTEAYDQ VTMATRALSP MRRLGGGLVE IPRVPERDPL 100
    TALMTNPVVA ESKRFCWNCG KPVGRSTPDG RALSEGWCPH CGSPYSFLPQ 150
    LSPGDIVADQ YEIKGCIAHG GLGWVYLAFD KNVNDRPVVL KGLVHSGDAE 200
    AQAIAMAERQ FLAEVTHPGI VKIYNFVEHE DKHGNPVGYI VMEYVGGTSL 250
    KQARGAKLPV AEAIGYMLEI LPALGYLHSI GLAYNDLKPE NIMITEEQLK 300
    LIDLGAVSRL NSYGYLYGTP GYQAPEIVRT GPTVATDIYT VGRTLAALTL 350
    SLRTRRGRYV DGLPSDDPVL ETYDSYHRLL RRAIDPDPRR RFTSAEEMSS 400
    QLLGVLREVV ATDTGVPRPG LSTVFSPSRS TFGVDLLVAH TDVYVDGQVH 450
    SEKLTAQEIV RALPVPLVDR TDVGAPMLVA SVLSEPVHTL DQLRAARHGA 500
    LDTEGIDLNE SVELPLMEVR ALLDLGDVAK ATRKLEDLAA RVGWRWRLVW 550
    FKAVSEMLSA DYDSATKHFT EVLDTLPGEL APKLALAATA ELAGTADELK 600
    FYKTVWSTDN GVISAGFGLA RAQSVAGERD MAVQTLDEVP PTSRHFTTAR 650
    LTSAVTLLSG RSTSEITEQH IRDAARRVEA LPDSEPRVLQ IRALVLGTAL 700
    DWLADNTASS NHILGFPFTE HGLKLGVEAS LRALARIAPT QSHRYALVDL 750
    ANSVRPMSTF 760
    Length:760
    Mass (Da):82,645
    Last modified:January 9, 2007 - v1
    Checksum:i417893787A19511B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71095.1.
    CP001663 Genomic DNA. Translation: AFP37250.1.
    RefSeqiYP_006565545.1. NC_018289.1.
    YP_885191.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK71095; ABK71095; MSMEG_0786.
    AFP37250; AFP37250; MSMEI_0770.
    GeneIDi4537074.
    KEGGimsg:MSMEI_0770.
    msm:MSMEG_0786.
    PATRICi18074040. VBIMycSme59918_0781.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK71095.1 .
    CP001663 Genomic DNA. Translation: AFP37250.1 .
    RefSeqi YP_006565545.1. NC_018289.1.
    YP_885191.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QQK3.
    SMRi A0QQK3. Positions 84-756.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi A0QQK3. 1 interaction.
    STRINGi 246196.MSMEG_0786.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK71095 ; ABK71095 ; MSMEG_0786 .
    AFP37250 ; AFP37250 ; MSMEI_0770 .
    GeneIDi 4537074.
    KEGGi msg:MSMEI_0770.
    msm:MSMEG_0786.
    PATRICi 18074040. VBIMycSme59918_0781.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000049016.
    KOi K14949.
    OMAi HRYALVD.
    OrthoDBi EOG6B35XT.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-786-MONOMER.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. Cited for: INTERACTION WITH GARA.
      Strain: ATCC 700084 / mc(2)155.
    5. "Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria."
      Houben E.N., Walburger A., Ferrari G., Nguyen L., Thompson C.J., Miess C., Vogel G., Mueller B., Pieters J.
      Mol. Microbiol. 72:41-52(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, LACK OF TRANSLATION IN VIVO.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiPKNG_MYCS2
    AccessioniPrimary (citable) accession number: A0QQK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    When artificially expressed from expression vectors, encodes an active kinase, which is able to block the lysosomal delivery of mycobacteria inside macrophages. However, while PknG transcripts are found in M.smegmatis, expression of PknG is blocked on a translational level in this non-pathogenic species. This lack of efficient translation is caused by regulatory elements in the upstream region of the gene (PubMed:19210624).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3