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A0QQK1

- ACKA_MYCS2

UniProt

A0QQK1 - ACKA_MYCS2

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Protein

Acetate kinase

Gene
ackA, MSMEG_0784, MSMEI_0768
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction By similarity.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+. Can also accept Mn2+ By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium By similarity
Binding sitei14 – 141ATP By similarity
Binding sitei71 – 711Substrate By similarity
Active sitei128 – 1281Proton donor/acceptor By similarity
Sitei160 – 1601Transition state stabilizer By similarity
Sitei221 – 2211Transition state stabilizer By similarity
Metal bindingi364 – 3641Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi188 – 1925ATPUniRule annotation
Nucleotide bindingi262 – 2643ATPUniRule annotation
Nucleotide bindingi310 – 3145ATPUniRule annotation

GO - Molecular functioni

  1. acetate kinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway
  2. organic acid metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-784-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinase (EC:2.7.2.1)
Alternative name(s):
Acetokinase
Gene namesi
Name:ackA
Ordered Locus Names:MSMEG_0784, MSMEI_0768
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Acetate kinaseUniRule annotationPRO_0000421948Add
BLAST

Interactioni

Subunit structurei

Homodimer Inferred.1 Publication

Protein-protein interaction databases

STRINGi246196.MSMEG_0784.

Structurei

Secondary structure

1
376
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87
Beta strandi13 – 186
Turni20 – 223
Beta strandi25 – 317
Helixi42 – 5514
Helixi60 – 623
Beta strandi65 – 739
Turni76 – 783
Helixi87 – 959
Helixi97 – 993
Turni101 – 1033
Helixi104 – 11714
Beta strandi123 – 1275
Helixi130 – 1323
Helixi137 – 1404
Helixi146 – 1516
Helixi161 – 17414
Helixi179 – 1813
Beta strandi183 – 19917
Beta strandi202 – 2076
Beta strandi214 – 2163
Helixi227 – 2359
Helixi241 – 25010
Helixi253 – 2586
Helixi263 – 2719
Helixi275 – 29925
Beta strandi304 – 3085
Helixi309 – 3146
Helixi316 – 3238
Helixi327 – 3293
Helixi335 – 3395
Beta strandi354 – 3596
Helixi364 – 3729

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IJNX-ray1.70A/B2-376[»]
ProteinModelPortaliA0QQK1.

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.

Phylogenomic databases

eggNOGiCOG0282.
HOGENOMiHOG000288399.
KOiK00925.
OMAiMIARETV.
OrthoDBiEOG69975F.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QQK1-1 [UniParc]FASTAAdd to Basket

« Hide

MTVLVVNSGS SSLKYAVVRP ASGEFLADGI IEEIGSGAVP DHDAALRAAF    50
DELAAAGLHL EDLDLKAVGH RMVHGGKTFY KPSVVDDELI AKARELSPLA 100
PLHNPPAIKG IEVARKLLPD LPHIAVFDTA FFHDLPAPAS TYAIDRELAE 150
TWHIKRYGFH GTSHEYVSQQ AAIFLDRPLE SLNQIVLHLG NGASASAVAG 200
GKAVDTSMGL TPMEGLVMGT RSGDIDPGVI MYLWRTAGMS VDDIESMLNR 250
RSGVLGLGGA SDFRKLRELI ESGDEHAKLA YDVYIHRLRK YIGAYMAVLG 300
RTDVISFTAG VGENVPPVRR DALAGLGGLG IEIDDALNSA KSDEPRLIST 350
PDSRVTVLVV PTNEELAIAR ACVGVV 376
Length:376
Mass (Da):40,116
Last modified:January 9, 2007 - v1
Checksum:i5F9BBCA380D2A161
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK74906.1.
CP001663 Genomic DNA. Translation: AFP37248.1.
RefSeqiYP_006565543.1. NC_018289.1.
YP_885189.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74906; ABK74906; MSMEG_0784.
AFP37248; AFP37248; MSMEI_0768.
GeneIDi4531693.
KEGGimsg:MSMEI_0768.
msm:MSMEG_0784.
PATRICi18074036. VBIMycSme59918_0779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK74906.1 .
CP001663 Genomic DNA. Translation: AFP37248.1 .
RefSeqi YP_006565543.1. NC_018289.1.
YP_885189.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IJN X-ray 1.70 A/B 2-376 [» ]
ProteinModelPortali A0QQK1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_0784.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK74906 ; ABK74906 ; MSMEG_0784 .
AFP37248 ; AFP37248 ; MSMEI_0768 .
GeneIDi 4531693.
KEGGi msg:MSMEI_0768.
msm:MSMEG_0784.
PATRICi 18074036. VBIMycSme59918_0779.

Phylogenomic databases

eggNOGi COG0282.
HOGENOMi HOG000288399.
KOi K00925.
OMAi MIARETV.
OrthoDBi EOG69975F.

Enzyme and pathway databases

UniPathwayi UPA00340 ; UER00458 .
BioCyci MSME246196:GJ4Y-784-MONOMER.

Family and domain databases

HAMAPi MF_00020. Acetate_kinase.
InterProi IPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view ]
PANTHERi PTHR21060. PTHR21060. 1 hit.
Pfami PF00871. Acetate_kinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSi PR00471. ACETATEKNASE.
TIGRFAMsi TIGR00016. ackA. 1 hit.
PROSITEi PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Crystal structure of an acetate kinase from Mycobacterium smegmatis bound to AMP and sulfate."
    Seattle structural genomics center for infectious disease (SSGCID)
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH ATP ANALOG AMP, SUBUNIT.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiACKA_MYCS2
AccessioniPrimary (citable) accession number: A0QQK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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