Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A0QQK1

- ACKA_MYCS2

UniProt

A0QQK1 - ACKA_MYCS2

Protein

Acetate kinase

Gene

ackA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

    Catalytic activityi

    ATP + acetate = ADP + acetyl phosphate.UniRule annotation

    Cofactori

    Mg2+. Can also accept Mn2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Binding sitei14 – 141ATPUniRule annotation
    Binding sitei71 – 711SubstrateUniRule annotation
    Active sitei128 – 1281Proton donor/acceptorUniRule annotation
    Sitei160 – 1601Transition state stabilizerUniRule annotation
    Sitei221 – 2211Transition state stabilizerUniRule annotation
    Metal bindingi364 – 3641MagnesiumUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi188 – 1925ATP
    Nucleotide bindingi262 – 2643ATP
    Nucleotide bindingi310 – 3145ATP

    GO - Molecular functioni

    1. acetate kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway
    2. organic acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-784-MONOMER.
    UniPathwayiUPA00340; UER00458.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
    Alternative name(s):
    AcetokinaseUniRule annotation
    Gene namesi
    Name:ackAUniRule annotation
    Ordered Locus Names:MSMEG_0784, MSMEI_0768
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 376376Acetate kinasePRO_0000421948Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0784.

    Structurei

    Secondary structure

    1
    376
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Beta strandi13 – 186
    Turni20 – 223
    Beta strandi25 – 317
    Helixi42 – 5514
    Helixi60 – 623
    Beta strandi65 – 739
    Turni76 – 783
    Helixi87 – 959
    Helixi97 – 993
    Turni101 – 1033
    Helixi104 – 11714
    Beta strandi123 – 1275
    Helixi130 – 1323
    Helixi137 – 1404
    Helixi146 – 1516
    Helixi161 – 17414
    Helixi179 – 1813
    Beta strandi183 – 19917
    Beta strandi202 – 2076
    Beta strandi214 – 2163
    Helixi227 – 2359
    Helixi241 – 25010
    Helixi253 – 2586
    Helixi263 – 2719
    Helixi275 – 29925
    Beta strandi304 – 3085
    Helixi309 – 3146
    Helixi316 – 3238
    Helixi327 – 3293
    Helixi335 – 3395
    Beta strandi354 – 3596
    Helixi364 – 3729

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IJNX-ray1.70A/B2-376[»]
    ProteinModelPortaliA0QQK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acetokinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0282.
    HOGENOMiHOG000288399.
    KOiK00925.
    OMAiMIARETV.
    OrthoDBiEOG69975F.

    Family and domain databases

    HAMAPiMF_00020. Acetate_kinase.
    InterProiIPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    IPR023865. Aliphatic_acid_kinase_CS.
    [Graphical view]
    PANTHERiPTHR21060. PTHR21060. 1 hit.
    PfamiPF00871. Acetate_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
    PRINTSiPR00471. ACETATEKNASE.
    TIGRFAMsiTIGR00016. ackA. 1 hit.
    PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
    PS01076. ACETATE_KINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QQK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVLVVNSGS SSLKYAVVRP ASGEFLADGI IEEIGSGAVP DHDAALRAAF    50
    DELAAAGLHL EDLDLKAVGH RMVHGGKTFY KPSVVDDELI AKARELSPLA 100
    PLHNPPAIKG IEVARKLLPD LPHIAVFDTA FFHDLPAPAS TYAIDRELAE 150
    TWHIKRYGFH GTSHEYVSQQ AAIFLDRPLE SLNQIVLHLG NGASASAVAG 200
    GKAVDTSMGL TPMEGLVMGT RSGDIDPGVI MYLWRTAGMS VDDIESMLNR 250
    RSGVLGLGGA SDFRKLRELI ESGDEHAKLA YDVYIHRLRK YIGAYMAVLG 300
    RTDVISFTAG VGENVPPVRR DALAGLGGLG IEIDDALNSA KSDEPRLIST 350
    PDSRVTVLVV PTNEELAIAR ACVGVV 376
    Length:376
    Mass (Da):40,116
    Last modified:January 9, 2007 - v1
    Checksum:i5F9BBCA380D2A161
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74906.1.
    CP001663 Genomic DNA. Translation: AFP37248.1.
    RefSeqiYP_006565543.1. NC_018289.1.
    YP_885189.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK74906; ABK74906; MSMEG_0784.
    AFP37248; AFP37248; MSMEI_0768.
    GeneIDi4531693.
    KEGGimsg:MSMEI_0768.
    msm:MSMEG_0784.
    PATRICi18074036. VBIMycSme59918_0779.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74906.1 .
    CP001663 Genomic DNA. Translation: AFP37248.1 .
    RefSeqi YP_006565543.1. NC_018289.1.
    YP_885189.1. NC_008596.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IJN X-ray 1.70 A/B 2-376 [» ]
    ProteinModelPortali A0QQK1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_0784.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK74906 ; ABK74906 ; MSMEG_0784 .
    AFP37248 ; AFP37248 ; MSMEI_0768 .
    GeneIDi 4531693.
    KEGGi msg:MSMEI_0768.
    msm:MSMEG_0784.
    PATRICi 18074036. VBIMycSme59918_0779.

    Phylogenomic databases

    eggNOGi COG0282.
    HOGENOMi HOG000288399.
    KOi K00925.
    OMAi MIARETV.
    OrthoDBi EOG69975F.

    Enzyme and pathway databases

    UniPathwayi UPA00340 ; UER00458 .
    BioCyci MSME246196:GJ4Y-784-MONOMER.

    Family and domain databases

    HAMAPi MF_00020. Acetate_kinase.
    InterProi IPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    IPR023865. Aliphatic_acid_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR21060. PTHR21060. 1 hit.
    Pfami PF00871. Acetate_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000722. Acetate_prop_kin. 1 hit.
    PRINTSi PR00471. ACETATEKNASE.
    TIGRFAMsi TIGR00016. ackA. 1 hit.
    PROSITEi PS01075. ACETATE_KINASE_1. 1 hit.
    PS01076. ACETATE_KINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Crystal structure of an acetate kinase from Mycobacterium smegmatis bound to AMP and sulfate."
      Seattle structural genomics center for infectious disease (SSGCID)
      Submitted (JAN-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH THE ATP ANALOG AMP, SUBUNIT.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiACKA_MYCS2
    AccessioniPrimary (citable) accession number: A0QQK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3