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Protein

Acetate kinase

Gene

ackA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Binding sitei14ATPUniRule annotation1
Binding sitei71SubstrateUniRule annotation1
Active sitei128Proton donor/acceptorUniRule annotation1
Sitei160Transition state stabilizerUniRule annotation1
Sitei221Transition state stabilizerUniRule annotation1
Metal bindingi364MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi188 – 192ATP5
Nucleotide bindingi262 – 264ATP3
Nucleotide bindingi310 – 314ATP5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:MSMEG_0784, MSMEI_0768
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004219481 – 376Acetate kinaseAdd BLAST376

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi246196.MSMEG_0784.

Structurei

Secondary structure

1376
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Beta strandi13 – 18Combined sources6
Turni20 – 22Combined sources3
Beta strandi25 – 31Combined sources7
Helixi42 – 55Combined sources14
Helixi60 – 62Combined sources3
Beta strandi65 – 73Combined sources9
Turni76 – 78Combined sources3
Helixi87 – 95Combined sources9
Helixi97 – 99Combined sources3
Turni101 – 103Combined sources3
Helixi104 – 117Combined sources14
Beta strandi123 – 127Combined sources5
Helixi130 – 132Combined sources3
Helixi137 – 140Combined sources4
Helixi146 – 151Combined sources6
Helixi161 – 174Combined sources14
Helixi179 – 181Combined sources3
Beta strandi183 – 199Combined sources17
Beta strandi202 – 207Combined sources6
Beta strandi214 – 216Combined sources3
Helixi227 – 235Combined sources9
Helixi241 – 250Combined sources10
Helixi253 – 258Combined sources6
Helixi263 – 271Combined sources9
Helixi275 – 299Combined sources25
Beta strandi304 – 308Combined sources5
Helixi309 – 314Combined sources6
Helixi316 – 323Combined sources8
Helixi327 – 329Combined sources3
Helixi335 – 339Combined sources5
Beta strandi354 – 359Combined sources6
Helixi364 – 372Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IJNX-ray1.70A/B2-376[»]
ProteinModelPortaliA0QQK1.
SMRiA0QQK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QQK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLVVNSGS SSLKYAVVRP ASGEFLADGI IEEIGSGAVP DHDAALRAAF
60 70 80 90 100
DELAAAGLHL EDLDLKAVGH RMVHGGKTFY KPSVVDDELI AKARELSPLA
110 120 130 140 150
PLHNPPAIKG IEVARKLLPD LPHIAVFDTA FFHDLPAPAS TYAIDRELAE
160 170 180 190 200
TWHIKRYGFH GTSHEYVSQQ AAIFLDRPLE SLNQIVLHLG NGASASAVAG
210 220 230 240 250
GKAVDTSMGL TPMEGLVMGT RSGDIDPGVI MYLWRTAGMS VDDIESMLNR
260 270 280 290 300
RSGVLGLGGA SDFRKLRELI ESGDEHAKLA YDVYIHRLRK YIGAYMAVLG
310 320 330 340 350
RTDVISFTAG VGENVPPVRR DALAGLGGLG IEIDDALNSA KSDEPRLIST
360 370
PDSRVTVLVV PTNEELAIAR ACVGVV
Length:376
Mass (Da):40,116
Last modified:January 9, 2007 - v1
Checksum:i5F9BBCA380D2A161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74906.1.
CP001663 Genomic DNA. Translation: AFP37248.1.
RefSeqiWP_011727188.1. NZ_CP009494.1.
YP_885189.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74906; ABK74906; MSMEG_0784.
AFP37248; AFP37248; MSMEI_0768.
GeneIDi4531693.
KEGGimsb:LJ00_03895.
msg:MSMEI_0768.
msm:MSMEG_0784.
PATRICi18074036. VBIMycSme59918_0779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74906.1.
CP001663 Genomic DNA. Translation: AFP37248.1.
RefSeqiWP_011727188.1. NZ_CP009494.1.
YP_885189.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IJNX-ray1.70A/B2-376[»]
ProteinModelPortaliA0QQK1.
SMRiA0QQK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_0784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74906; ABK74906; MSMEG_0784.
AFP37248; AFP37248; MSMEI_0768.
GeneIDi4531693.
KEGGimsb:LJ00_03895.
msg:MSMEI_0768.
msm:MSMEG_0784.
PATRICi18074036. VBIMycSme59918_0779.

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACKA_MYCS2
AccessioniPrimary (citable) accession number: A0QQK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.