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Protein

Phosphate-import protein PhnD

Gene

phnD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PhnCDE involved in phosphate import. Responsible for phosphate binding.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Transport

Protein family/group databases

TCDBi3.A.1.9.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate-import protein PhnD
Gene namesi
Name:phnD
Ordered Locus Names:MSMEG_0649, MSMEI_0632
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22PROSITE-ProRule annotationAdd BLAST22
ChainiPRO_000035747023 – 321Phosphate-import protein PhnDAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi23N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi23S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

By phosphate-limited conditions, via derepression by PhnF, and probably also via the two-component regulatory system senX3/regX3.1 Publication

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_0649.

Structurei

3D structure databases

ProteinModelPortaliA0QQ71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106ZX3. Bacteria.
COG3221. LUCA.
HOGENOMiHOG000284940.
KOiK02044.
OMAiSSHEVNA.
OrthoDBiPOG091H0I0J.

Family and domain databases

InterProiIPR005770. PhnD.
[Graphical view]
TIGRFAMsiTIGR01098. 3A0109s03R. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QQ71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRAHHKIA TAAACVALLA SACSGSDKPQ STTAEGFPET ITLAAIPAEN
60 70 80 90 100
SSDLKASYDP LIKMLEKQTG SKVEFVQASD YAGVVEGMIA GNVDLAFFGP
110 120 130 140 150
FAYVVAGVNG AKMTPLGAVI KDEGGAPGYQ SYGLARADED NINGLKDFAG
160 170 180 190 200
KKVCFVDPGS TSGFLYPTAG LIEEGVVKSG SEADISAAMS PIFAGGHDSS
210 220 230 240 250
ALAIANGDCD AGFAFDTMVD KTMIDKGDLK PGQLKTVWKS DMIAGSVFAA
260 270 280 290 300
NDALGPEVID KLKTMFAQDA NVKSFEEEGF CEGDACRITD ERAWGVVPVT
310 320
DADYDGVRHV CDVTGSEKCK G
Length:321
Mass (Da):33,385
Last modified:January 9, 2007 - v1
Checksum:iB4B1D2809903748D
GO

Sequence cautioni

The sequence AFP37113 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73977.1.
CP001663 Genomic DNA. Translation: AFP37113.1. Different initiation.
RefSeqiWP_003892069.1. NZ_CP009494.1.
YP_885059.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73977; ABK73977; MSMEG_0649.
AFP37113; AFP37113; MSMEI_0632.
GeneIDi4536326.
KEGGimsb:LJ00_03220.
msg:MSMEI_0632.
msm:MSMEG_0649.
PATRICi18073766. VBIMycSme59918_0645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73977.1.
CP001663 Genomic DNA. Translation: AFP37113.1. Different initiation.
RefSeqiWP_003892069.1. NZ_CP009494.1.
YP_885059.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QQ71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_0649.

Protein family/group databases

TCDBi3.A.1.9.2. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73977; ABK73977; MSMEG_0649.
AFP37113; AFP37113; MSMEI_0632.
GeneIDi4536326.
KEGGimsb:LJ00_03220.
msg:MSMEI_0632.
msm:MSMEG_0649.
PATRICi18073766. VBIMycSme59918_0645.

Phylogenomic databases

eggNOGiENOG4106ZX3. Bacteria.
COG3221. LUCA.
HOGENOMiHOG000284940.
KOiK02044.
OMAiSSHEVNA.
OrthoDBiPOG091H0I0J.

Family and domain databases

InterProiIPR005770. PhnD.
[Graphical view]
TIGRFAMsiTIGR01098. 3A0109s03R. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHND_MYCS2
AccessioniPrimary (citable) accession number: A0QQ71
Secondary accession number(s): I7FDW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.