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Protein

Trehalose 2-sulfotransferase

Gene

stf0

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sulfuryl group transfer from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid in pathogenic mycobacteria. Cannot use free glucose and unnatural stereoisomers of trehalose (alpha,beta (neo-trehalose) and beta,beta (iso-trehalose)) as substrates.1 Publication

Miscellaneous

The reaction catalyzed by Stf0 follows a rapid equilibrium random sequential Bi-Bi mechanism with formation of a ternary complex intermediate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose.1 Publication

Kineticsi

kcat is 1.6 sec(-1) (PubMed:15258569). kcat is 134 min(-1) toward trehalose and 96 min(-1) toward PAPS (PubMed:15866533).2 Publications
  1. KM=18 µM for 3'-phosphoadenylyl sulfate1 Publication
  2. KM=18 mM for alpha,alpha-trehalose1 Publication
  3. KM=15 mM for alpha,alpha-trehalose1 Publication

    Pathwayi: Glycolipid metabolism

    This protein is involved in Glycolipid metabolism.1 Publication
    View all proteins of this organism that are known to be involved in Glycolipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei14alpha,alpha-trehaloseCombined sources1 Publication1
    Active sitei36Proton acceptor1 Publication1
    Binding sitei48alpha,alpha-trehalose; via carbonyl oxygenCombined sources1 Publication1
    Binding sitei53alpha,alpha-trehaloseCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processCarbohydrate metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trehalose 2-sulfotransferase1 Publication (EC:2.8.2.371 Publication)
    Gene namesi
    Name:stf01 Publication
    Ordered Locus Names:MSMEG_0630Imported, MSMEI_0614Imported
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
    Proteomesi
    • UP000006158 Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Loss of trehalose-2-sulfate (T2S) formation.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi33E → A: 4-fold decrease in catalytic efficiency due to a reduction in both trehalose affinity and reaction rate. 1 Publication1
    Mutagenesisi36E → A: 100-fold decrease in reaction rate and 3-fold increase in trehalose affinity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004347831 – 267Trehalose 2-sulfotransferaseAdd BLAST267

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0630.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1TEXX-ray2.60A/B/C/D1-267[»]
    ProteinModelPortaliA0QQ53.
    SMRiA0QQ53.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni33 – 39alpha,alpha-trehalose bindingCombined sources1 Publication7

    Sequence similaritiesi

    Belongs to the Stf0 sulfotransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG4424. LUCA.
    HOGENOMiHOG000070040.
    KOiK21014.
    OMAiAVSMWRA.
    OrthoDBiPOG091H08HB.

    Family and domain databases

    InterProiView protein in InterPro
    IPR027417. P-loop_NTPase.
    IPR015124. Stf0.
    IPR024628. Sulfotransferase_Stf0_dom.
    PfamiView protein in Pfam
    PF09037. Sulphotransf. 1 hit.
    PIRSFiPIRSF021497. Sulphotransferase_Stf0. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0QQ53-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSDHPTAYLV LASQRSGSTL LVESLRATGV AGEPQEFFQY LPNTSMSPQP
    60 70 80 90 100
    REWFADVEDQ SILRLLDPLI EGKPDLAPAT IWRDYIQTVG RTPNGVWGGK
    110 120 130 140 150
    LMWNQTPLLV QRAKDLPDRS GSGLLSAIRD VVGSDPVLIH IHRPDVVSQA
    160 170 180 190 200
    VSFWRAVQTR VWRGRPDPVR DARAEYHAGA IAHVITMLRA QEEGWRAWFT
    210 220 230 240 250
    EENVEPIDVD YPYLWRNLTE VVGTVLEALG QDPRLAPKPV LERQADQRSD
    260
    EWVERYRRDA QRDGLPL
    Length:267
    Mass (Da):30,291
    Last modified:January 9, 2007 - v1
    Checksum:i7432364D0ABB8956
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA. Translation: ABK69637.1.
    CP001663 Genomic DNA. Translation: AFP37095.1.
    RefSeqiWP_011727079.1. NZ_CP009494.1.
    YP_885041.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK69637; ABK69637; MSMEG_0630.
    AFP37095; AFP37095; MSMEI_0614.
    GeneIDi4536599.
    KEGGimsb:LJ00_03130.
    msg:MSMEI_0614.
    msm:MSMEG_0630.
    PATRICifig|246196.19.peg.626.

    Similar proteinsi

    Entry informationi

    Entry nameiSTF0_MYCS2
    AccessioniPrimary (citable) accession number: A0QQ53
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 11, 2015
    Last sequence update: January 9, 2007
    Last modified: July 5, 2017
    This is version 84 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families