Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A0QNG1

- PKNB_MYCS2

UniProt

A0QNG1 - PKNB_MYCS2

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins (By similarity). Probably phosphorylates RseA.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

By K-252a.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATPPROSITE-ProRule annotation
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
Metal bindingi143 – 1431MagnesiumBy similarity
Metal bindingi156 – 1561MagnesiumBy similarity
Binding sitei156 – 1561ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation
Nucleotide bindingi93 – 953ATPPROSITE-ProRule annotation
Nucleotide bindingi140 – 1434ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. penicillin binding Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-29-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:MSMEG_0028, MSMEI_0031
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

When depleted (with anti-sense RNA) no vancomycin-induced degradation of RseA is seen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Serine/threonine-protein kinase PknBPRO_0000422953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei169 – 1691Phosphoserine; by autocatalysisBy similarity
Modified residuei171 – 1711Phosphothreonine; by autocatalysisBy similarity
Modified residuei173 – 1731Phosphothreonine; by autocatalysisBy similarity
Modified residuei294 – 2941Phosphothreonine; by autocatalysisBy similarity
Modified residuei295 – 2951Phosphoserine; by autocatalysisBy similarity
Modified residuei309 – 3091Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_0028.

Structurei

3D structure databases

ProteinModelPortaliA0QNG1.
SMRiA0QNG1. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 331331CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini353 – 625273ExtracellularSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 274264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini355 – 42167PASTA 1PROSITE-ProRule annotationAdd
BLAST
Domaini422 – 48968PASTA 2PROSITE-ProRule annotationAdd
BLAST
Domaini490 – 55667PASTA 3PROSITE-ProRule annotationAdd
BLAST
Domaini557 – 62569PASTA 4PROSITE-ProRule annotationAdd
BLAST

Domaini

The PASTA domains interact with peptidoglycans and are required for PknB localization.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037185.
KOiK08884.
OMAiMSKNPAN.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QNG1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPQHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPNGPLPYI VMEYVDGVTL
110 120 130 140 150
RDIVHTDGPI APRRAIEIIA DACQALNFSH QHGIIHRDVK PANIMISKNN
160 170 180 190 200
AVKVMDFGIA RALADTGNSV TQTAAVIGTA QYLSPEQARG ETVDARSDVY
210 220 230 240 250
SLGCVLYEIL TGEPPFIGDS PVAVAYQHVR EDPVPPSRRH ADVTPELDAV
260 270 280 290 300
VLKALAKNPD NRYQTAAEMR ADLIRVHEGQ APDAPKVLTD AERTSMLAAP
310 320 330 340 350
PADRAGAATQ DMPVPRPAGY SKQRSTSVAR WLIAVAVLAV LTVVVTVAIN
360 370 380 390 400
MVGGNPRNVQ VPDVAEQSAD DAQAALQNRG FKTVIDRQPD NEVPPGLVIG
410 420 430 440 450
TDPEAGSELG AGEQVTINVS TGPEQALVPD VAGLTPTQAR QKLKDAGFEK
460 470 480 490 500
FRESPSPSTP EQKGRVLATN PQANQTAAII NEITIVVGAG PEDAPVLSCA
510 520 530 540 550
GQNAESCKAI LAAGGFTNTV VVEVDNPAAA GQVVGTEPAD GQSVPKDTVI
560 570 580 590 600
QIRVSKGNQF VMPDLVGQFW SDAYPRLTAL GWTGVLDKGP DVRDSGQRTN
610 620
AVVTQSPSAG TPVNKDAKIT LSFAA
Length:625
Mass (Da):66,318
Last modified:January 9, 2007 - v1
Checksum:iE9B75950ADF718DA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK74027.1.
CP001663 Genomic DNA. Translation: AFP36514.1.
RefSeqiYP_006564809.1. NC_018289.1.
YP_884449.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74027; ABK74027; MSMEG_0028.
AFP36514; AFP36514; MSMEI_0031.
GeneIDi4536298.
KEGGimsg:MSMEI_0031.
msm:MSMEG_0028.
PATRICi18072526. VBIMycSme59918_0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK74027.1 .
CP001663 Genomic DNA. Translation: AFP36514.1 .
RefSeqi YP_006564809.1. NC_018289.1.
YP_884449.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0QNG1.
SMRi A0QNG1. Positions 1-286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_0028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK74027 ; ABK74027 ; MSMEG_0028 .
AFP36514 ; AFP36514 ; MSMEI_0031 .
GeneIDi 4536298.
KEGGi msg:MSMEI_0031.
msm:MSMEG_0028.
PATRICi 18072526. VBIMycSme59918_0027.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000037185.
KOi K08884.
OMAi MSKNPAN.
OrthoDBi EOG6B35XT.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-29-MONOMER.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis."
    Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.
    Mol. Microbiol. 75:592-606(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE FUNCTION AS A KINASE WITH RSEA AS A SUBSTRATE, ENZYME REGULATION, DISRUPTION PHENOTYPE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPKNB_MYCS2
AccessioniPrimary (citable) accession number: A0QNG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: January 9, 2007
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3