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A0QNG1

- PKNB_MYCS2

UniProt

A0QNG1 - PKNB_MYCS2

Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins By similarity. Probably phosphorylates RseA.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    By K-252a.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401ATPPROSITE-ProRule annotation
    Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
    Metal bindingi143 – 1431MagnesiumBy similarity
    Metal bindingi156 – 1561MagnesiumBy similarity
    Binding sitei156 – 1561ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation
    Nucleotide bindingi93 – 953ATPPROSITE-ProRule annotation
    Nucleotide bindingi140 – 1434ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. penicillin binding Source: InterPro
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-29-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase PknB (EC:2.7.11.1)
    Gene namesi
    Name:pknB
    Ordered Locus Names:MSMEG_0028, MSMEI_0031
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    When depleted (with anti-sense RNA) no vancomycin-induced degradation of RseA is seen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625Serine/threonine-protein kinase PknBPRO_0000422953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei169 – 1691Phosphoserine; by autocatalysisBy similarity
    Modified residuei171 – 1711Phosphothreonine; by autocatalysisBy similarity
    Modified residuei173 – 1731Phosphothreonine; by autocatalysisBy similarity
    Modified residuei294 – 2941Phosphothreonine; by autocatalysisBy similarity
    Modified residuei295 – 2951Phosphoserine; by autocatalysisBy similarity
    Modified residuei309 – 3091Phosphothreonine; by autocatalysisBy similarity

    Post-translational modificationi

    Autophosphorylated. Dephosphorylated by PstP By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0028.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QNG1.
    SMRiA0QNG1. Positions 1-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 331331CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini353 – 625273ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei332 – 35221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 274264Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini355 – 42167PASTA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini422 – 48968PASTA 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini490 – 55667PASTA 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini557 – 62569PASTA 4PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The PASTA domains interact with peptidoglycans and are required for PknB localization.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 4 PASTA domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000037185.
    KOiK08884.
    OMAiMSKNPAN.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QNG1-1 [UniParc]FASTAAdd to Basket

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    MTTPQHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP    50
    SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPNGPLPYI VMEYVDGVTL 100
    RDIVHTDGPI APRRAIEIIA DACQALNFSH QHGIIHRDVK PANIMISKNN 150
    AVKVMDFGIA RALADTGNSV TQTAAVIGTA QYLSPEQARG ETVDARSDVY 200
    SLGCVLYEIL TGEPPFIGDS PVAVAYQHVR EDPVPPSRRH ADVTPELDAV 250
    VLKALAKNPD NRYQTAAEMR ADLIRVHEGQ APDAPKVLTD AERTSMLAAP 300
    PADRAGAATQ DMPVPRPAGY SKQRSTSVAR WLIAVAVLAV LTVVVTVAIN 350
    MVGGNPRNVQ VPDVAEQSAD DAQAALQNRG FKTVIDRQPD NEVPPGLVIG 400
    TDPEAGSELG AGEQVTINVS TGPEQALVPD VAGLTPTQAR QKLKDAGFEK 450
    FRESPSPSTP EQKGRVLATN PQANQTAAII NEITIVVGAG PEDAPVLSCA 500
    GQNAESCKAI LAAGGFTNTV VVEVDNPAAA GQVVGTEPAD GQSVPKDTVI 550
    QIRVSKGNQF VMPDLVGQFW SDAYPRLTAL GWTGVLDKGP DVRDSGQRTN 600
    AVVTQSPSAG TPVNKDAKIT LSFAA 625
    Length:625
    Mass (Da):66,318
    Last modified:January 9, 2007 - v1
    Checksum:iE9B75950ADF718DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74027.1.
    CP001663 Genomic DNA. Translation: AFP36514.1.
    RefSeqiYP_006564809.1. NC_018289.1.
    YP_884449.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK74027; ABK74027; MSMEG_0028.
    AFP36514; AFP36514; MSMEI_0031.
    GeneIDi4536298.
    KEGGimsg:MSMEI_0031.
    msm:MSMEG_0028.
    PATRICi18072526. VBIMycSme59918_0027.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74027.1 .
    CP001663 Genomic DNA. Translation: AFP36514.1 .
    RefSeqi YP_006564809.1. NC_018289.1.
    YP_884449.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QNG1.
    SMRi A0QNG1. Positions 1-286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_0028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK74027 ; ABK74027 ; MSMEG_0028 .
    AFP36514 ; AFP36514 ; MSMEI_0031 .
    GeneIDi 4536298.
    KEGGi msg:MSMEI_0031.
    msm:MSMEG_0028.
    PATRICi 18072526. VBIMycSme59918_0027.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000037185.
    KOi K08884.
    OMAi MSKNPAN.
    OrthoDBi EOG6B35XT.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-29-MONOMER.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis."
      Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.
      Mol. Microbiol. 75:592-606(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROBABLE FUNCTION AS A KINASE WITH RSEA AS A SUBSTRATE, ENZYME REGULATION, DISRUPTION PHENOTYPE.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiPKNB_MYCS2
    AccessioniPrimary (citable) accession number: A0QNG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3