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Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that regulates many aspects of mycobacterial physiology. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins (By similarity). Probably phosphorylates RseA (PubMed:20025669).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

By K-252a.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1
Metal bindingi143MagnesiumBy similarity1
Metal bindingi156MagnesiumBy similarity1
Binding sitei156ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9
Nucleotide bindingi93 – 95ATPPROSITE-ProRule annotation3
Nucleotide bindingi140 – 143ATPPROSITE-ProRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:MSMEG_0028, MSMEI_0031
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 331CytoplasmicSequence analysisAdd BLAST331
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 625ExtracellularSequence analysisAdd BLAST273

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

When depleted (with anti-sense RNA) no vancomycin-induced degradation of RseA is seen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004229531 – 625Serine/threonine-protein kinase PknBAdd BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169Phosphoserine; by autocatalysisBy similarity1
Modified residuei171Phosphothreonine; by autocatalysisBy similarity1
Modified residuei173Phosphothreonine; by autocatalysisBy similarity1
Modified residuei294Phosphothreonine; by autocatalysisBy similarity1
Modified residuei295Phosphoserine; by autocatalysisBy similarity1
Modified residuei309Phosphothreonine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_0028.

Structurei

3D structure databases

ProteinModelPortaliA0QNG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 274Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini355 – 421PASTA 1PROSITE-ProRule annotationAdd BLAST67
Domaini422 – 489PASTA 2PROSITE-ProRule annotationAdd BLAST68
Domaini490 – 556PASTA 3PROSITE-ProRule annotationAdd BLAST67
Domaini557 – 625PASTA 4PROSITE-ProRule annotationAdd BLAST69

Domaini

The PASTA domains interact with peptidoglycans and are required for PknB localization.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D9P. Bacteria.
COG0515. LUCA.
COG2815. LUCA.
HOGENOMiHOG000037185.
KOiK12132.
OMAiIRMYIND.
OrthoDBiPOG091H0264.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QNG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPNGPLPYI VMEYVDGVTL
110 120 130 140 150
RDIVHTDGPI APRRAIEIIA DACQALNFSH QHGIIHRDVK PANIMISKNN
160 170 180 190 200
AVKVMDFGIA RALADTGNSV TQTAAVIGTA QYLSPEQARG ETVDARSDVY
210 220 230 240 250
SLGCVLYEIL TGEPPFIGDS PVAVAYQHVR EDPVPPSRRH ADVTPELDAV
260 270 280 290 300
VLKALAKNPD NRYQTAAEMR ADLIRVHEGQ APDAPKVLTD AERTSMLAAP
310 320 330 340 350
PADRAGAATQ DMPVPRPAGY SKQRSTSVAR WLIAVAVLAV LTVVVTVAIN
360 370 380 390 400
MVGGNPRNVQ VPDVAEQSAD DAQAALQNRG FKTVIDRQPD NEVPPGLVIG
410 420 430 440 450
TDPEAGSELG AGEQVTINVS TGPEQALVPD VAGLTPTQAR QKLKDAGFEK
460 470 480 490 500
FRESPSPSTP EQKGRVLATN PQANQTAAII NEITIVVGAG PEDAPVLSCA
510 520 530 540 550
GQNAESCKAI LAAGGFTNTV VVEVDNPAAA GQVVGTEPAD GQSVPKDTVI
560 570 580 590 600
QIRVSKGNQF VMPDLVGQFW SDAYPRLTAL GWTGVLDKGP DVRDSGQRTN
610 620
AVVTQSPSAG TPVNKDAKIT LSFAA
Length:625
Mass (Da):66,318
Last modified:January 9, 2007 - v1
Checksum:iE9B75950ADF718DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74027.1.
CP001663 Genomic DNA. Translation: AFP36514.1.
RefSeqiWP_003891355.1. NZ_CP009494.1.
YP_884449.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74027; ABK74027; MSMEG_0028.
AFP36514; AFP36514; MSMEI_0031.
GeneIDi4536298.
KEGGimsb:LJ00_00145.
msg:MSMEI_0031.
msm:MSMEG_0028.
PATRICi18072526. VBIMycSme59918_0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74027.1.
CP001663 Genomic DNA. Translation: AFP36514.1.
RefSeqiWP_003891355.1. NZ_CP009494.1.
YP_884449.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QNG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_0028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74027; ABK74027; MSMEG_0028.
AFP36514; AFP36514; MSMEI_0031.
GeneIDi4536298.
KEGGimsb:LJ00_00145.
msg:MSMEI_0031.
msm:MSMEG_0028.
PATRICi18072526. VBIMycSme59918_0027.

Phylogenomic databases

eggNOGiENOG4105D9P. Bacteria.
COG0515. LUCA.
COG2815. LUCA.
HOGENOMiHOG000037185.
KOiK12132.
OMAiIRMYIND.
OrthoDBiPOG091H0264.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKNB_MYCS2
AccessioniPrimary (citable) accession number: A0QNG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.