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A0QNG1

- PKNB_MYCS2

UniProt

A0QNG1 - PKNB_MYCS2

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Protein
Serine/threonine-protein kinase PknB
Gene
pknB, MSMEG_0028, MSMEI_0031
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins By similarity. Probably phosphorylates RseA.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

By K-252a Inferred.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATP By similarity
Active sitei138 – 1381Proton acceptor By similarity
Metal bindingi143 – 1431Magnesium By similarity
Metal bindingi156 – 1561Magnesium By similarity
Binding sitei156 – 1561ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATP By similarity
Nucleotide bindingi93 – 953ATP By similarity
Nucleotide bindingi140 – 1434ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. penicillin binding Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-29-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase PknB (EC:2.7.11.1)
    Gene namesi
    Name:pknB
    Ordered Locus Names:MSMEG_0028, MSMEI_0031
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 331331Cytoplasmic Reviewed prediction
    Add
    BLAST
    Transmembranei332 – 35221Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini353 – 625273Extracellular Reviewed prediction
    Add
    BLAST

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    When depleted (with anti-sense RNA) no vancomycin-induced degradation of RseA is seen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625Serine/threonine-protein kinase PknB
    PRO_0000422953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei169 – 1691Phosphoserine; by autocatalysis By similarity
    Modified residuei171 – 1711Phosphothreonine; by autocatalysis By similarity
    Modified residuei173 – 1731Phosphothreonine; by autocatalysis By similarity
    Modified residuei294 – 2941Phosphothreonine; by autocatalysis By similarity
    Modified residuei295 – 2951Phosphoserine; by autocatalysis By similarity
    Modified residuei309 – 3091Phosphothreonine; by autocatalysis By similarity

    Post-translational modificationi

    Autophosphorylated. Dephosphorylated by PstP By similarity.

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer By similarity.

    Protein-protein interaction databases

    STRINGi246196.MSMEG_0028.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QNG1.
    SMRiA0QNG1. Positions 1-286.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 274264Protein kinase
    Add
    BLAST
    Domaini355 – 42167PASTA 1
    Add
    BLAST
    Domaini422 – 48968PASTA 2
    Add
    BLAST
    Domaini490 – 55667PASTA 3
    Add
    BLAST
    Domaini557 – 62569PASTA 4
    Add
    BLAST

    Domaini

    The PASTA domains interact with peptidoglycans and are required for PknB localization By similarity.

    Sequence similaritiesi

    Contains 4 PASTA domains.

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000037185.
    KOiK08884.
    OMAiMSKNPAN.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QNG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPQHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP    50
    SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPNGPLPYI VMEYVDGVTL 100
    RDIVHTDGPI APRRAIEIIA DACQALNFSH QHGIIHRDVK PANIMISKNN 150
    AVKVMDFGIA RALADTGNSV TQTAAVIGTA QYLSPEQARG ETVDARSDVY 200
    SLGCVLYEIL TGEPPFIGDS PVAVAYQHVR EDPVPPSRRH ADVTPELDAV 250
    VLKALAKNPD NRYQTAAEMR ADLIRVHEGQ APDAPKVLTD AERTSMLAAP 300
    PADRAGAATQ DMPVPRPAGY SKQRSTSVAR WLIAVAVLAV LTVVVTVAIN 350
    MVGGNPRNVQ VPDVAEQSAD DAQAALQNRG FKTVIDRQPD NEVPPGLVIG 400
    TDPEAGSELG AGEQVTINVS TGPEQALVPD VAGLTPTQAR QKLKDAGFEK 450
    FRESPSPSTP EQKGRVLATN PQANQTAAII NEITIVVGAG PEDAPVLSCA 500
    GQNAESCKAI LAAGGFTNTV VVEVDNPAAA GQVVGTEPAD GQSVPKDTVI 550
    QIRVSKGNQF VMPDLVGQFW SDAYPRLTAL GWTGVLDKGP DVRDSGQRTN 600
    AVVTQSPSAG TPVNKDAKIT LSFAA 625
    Length:625
    Mass (Da):66,318
    Last modified:January 9, 2007 - v1
    Checksum:iE9B75950ADF718DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74027.1.
    CP001663 Genomic DNA. Translation: AFP36514.1.
    RefSeqiYP_006564809.1. NC_018289.1.
    YP_884449.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK74027; ABK74027; MSMEG_0028.
    AFP36514; AFP36514; MSMEI_0031.
    GeneIDi4536298.
    KEGGimsg:MSMEI_0031.
    msm:MSMEG_0028.
    PATRICi18072526. VBIMycSme59918_0027.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74027.1 .
    CP001663 Genomic DNA. Translation: AFP36514.1 .
    RefSeqi YP_006564809.1. NC_018289.1.
    YP_884449.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QNG1.
    SMRi A0QNG1. Positions 1-286.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_0028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK74027 ; ABK74027 ; MSMEG_0028 .
    AFP36514 ; AFP36514 ; MSMEI_0031 .
    GeneIDi 4536298.
    KEGGi msg:MSMEI_0031.
    msm:MSMEG_0028.
    PATRICi 18072526. VBIMycSme59918_0027.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000037185.
    KOi K08884.
    OMAi MSKNPAN.
    OrthoDBi EOG6B35XT.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-29-MONOMER.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "RseA, the SigE specific anti-sigma factor of Mycobacterium tuberculosis, is inactivated by phosphorylation-dependent ClpC1P2 proteolysis."
      Barik S., Sureka K., Mukherjee P., Basu J., Kundu M.
      Mol. Microbiol. 75:592-606(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROBABLE FUNCTION AS A KINASE WITH RSEA AS A SUBSTRATE, ENZYME REGULATION, DISRUPTION PHENOTYPE.
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiPKNB_MYCS2
    AccessioniPrimary (citable) accession number: A0QNG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: January 9, 2007
    Last modified: September 3, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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