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Protein

DNA gyrase subunit B

Gene

gyrB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner (PubMed:8878580) to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation1 Publication

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Enzyme regulationi

Inhibited by 4-quinoline drugs (nalidixic acid, ciprofloxacin, ofloxacin), although it is much less sensitive than the corresponding enzyme from E.coli (PubMed:8878580). GyrB intrinsic ATPase activity inhibited by aminopyrazinamide and pyrrolamide derivatives (PubMed:23268609, PubMed:24126580).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi459Magnesium 1; catalyticUniRule annotation1
Metal bindingi532Magnesium 1; catalyticUniRule annotation1
Metal bindingi532Magnesium 2UniRule annotation1
Metal bindingi534Magnesium 2UniRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA supercoiling activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrBUniRule annotation
Ordered Locus Names:MSMEG_0005, MSMEI_0007
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6059

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002935992 – 675DNA gyrase subunit BAdd BLAST674

Proteomic databases

PRIDEiA0QNE0

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains (PubMed:8878580). In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei484Interaction with DNAUniRule annotation1
Sitei487Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi246196.MSMEG_0005

Structurei

Secondary structure

1675
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 28Combined sources5
Helixi31 – 34Combined sources4
Helixi39 – 58Combined sources20
Beta strandi64 – 69Combined sources6
Beta strandi73 – 79Combined sources7
Beta strandi90 – 94Combined sources5
Helixi95 – 101Combined sources7
Helixi127 – 130Combined sources4
Beta strandi131 – 141Combined sources11
Beta strandi144 – 151Combined sources8
Beta strandi159 – 163Combined sources5
Beta strandi168 – 175Combined sources8
Turni177 – 179Combined sources3
Helixi187 – 200Combined sources14
Beta strandi205 – 210Combined sources6
Turni211 – 213Combined sources3
Beta strandi248 – 251Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B6CX-ray2.20A/B9-255[»]
4BAEX-ray2.35A/B/C/D19-212[»]
A/B/C/D247-255[»]
ProteinModelPortaliA0QNE0
SMRiA0QNE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini453 – 567ToprimUniRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the type II topoisomerase GyrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C7D Bacteria
COG0187 LUCA
HOGENOMiHOG000075154
KOiK02470
OMAiDCSSRDP
OrthoDBiPOG091H01XP

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
Gene3Di3.30.230.10, 1 hit
3.30.565.10, 1 hit
3.40.50.670, 1 hit
HAMAPiMF_01898 GyrB, 1 hit
InterProiView protein in InterPro
IPR002288 DNA_gyrase_B_C
IPR011557 GyrB
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR001241 Topo_IIA
IPR013760 Topo_IIA-like_dom_sf
IPR013759 Topo_IIA_B_C
IPR013506 Topo_IIA_bsu_dom2
IPR018522 TopoIIA_CS
IPR006171 TOPRIM_domain
PANTHERiPTHR10169 PTHR10169, 1 hit
PfamiView protein in Pfam
PF00204 DNA_gyraseB, 1 hit
PF00986 DNA_gyraseB_C, 1 hit
PF02518 HATPase_c, 1 hit
PF01751 Toprim, 1 hit
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00433 TOP2c, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
SSF56719 SSF56719, 1 hit
TIGRFAMsiTIGR01059 gyrB, 1 hit
PROSITEiView protein in PROSITE
PS00177 TOPOISOMERASE_II, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QNE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQKNNAPK EYGADSITIL EGLEAVRKRP GMYIGSTGER GLHHLIWEVV
60 70 80 90 100
DNAVDEAMAG FATRVDVKIH ADGSVEVRDD GRGIPVEMHA TGMPTIDVVM
110 120 130 140 150
TQLHAGGKFD GETYAVSGGL HGVGVSVVNA LSTRLEATVL RDGYEWFQYY
160 170 180 190 200
DRSVPGKLKQ GGETKETGTT IRFWADPEIF ETTDYNFETV ARRLQEMAFL
210 220 230 240 250
NKGLTIELTD ERVTAEEVVD DVVKDTAEAP KTADEKAAEA TGPSKVKHRV
260 270 280 290 300
FHYPGGLVDY VKHINRTKTP IQQSIIDFDG KGPGHEVEIA MQWNAGYSES
310 320 330 340 350
VHTFANTINT HEGGTHEEGF RAALTSVVNR YAKDKKLLKD KDPNLTGDDI
360 370 380 390 400
REGLAAVISV KVAEPQFEGQ TKTKLGNTEV KSFVQKICNE QLQHWFEANP
410 420 430 440 450
AEAKTVVNKA VSSAQARIAA RKARELVRRK SATDIGGLPG KLADCRSTDP
460 470 480 490 500
SKSELYVVEG DSAGGSAKSG RDSMFQAILP LRGKIINVEK ARIDRVLKNT
510 520 530 540 550
EVQSIITALG TGIHDEFDIS KLRYHKIVLM ADADVDGQHI STLLLTLLFR
560 570 580 590 600
FMKPLVENGH IFLAQPPLYK LKWQRSEPEF AYSDRERDGL LEAGRAAGKK
610 620 630 640 650
INVDDGIQRY KGLGEMDAKE LWETTMDPSV RVLRQVTLDD AAAADELFSI
660 670
LMGEDVEARR SFITRNAKDV RFLDV
Length:675
Mass (Da):74,512
Last modified:January 9, 2007 - v1
Checksum:i68A9B7F98BE07951
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442L → S in CAA63917 (PubMed:8878580).Curated1
Sequence conflicti507T → R in CAA63917 (PubMed:8878580).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94224 Genomic DNA Translation: CAA63917.1
CP000480 Genomic DNA Translation: ABK72750.1
CP001663 Genomic DNA Translation: AFP36492.1
RefSeqiWP_003891333.1, NZ_CP009494.1
YP_884428.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK72750; ABK72750; MSMEG_0005
AFP36492; AFP36492; MSMEI_0007
GeneIDi4531510
KEGGimsb:LJ00_00025
msg:MSMEI_0007
msm:MSMEG_0005
PATRICifig|246196.19.peg.5

Similar proteinsi

Entry informationi

Entry nameiGYRB_MYCS2
AccessioniPrimary (citable) accession number: A0QNE0
Secondary accession number(s): I7FVC1, P48355, Q59555
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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