A0QMH2 (ILVD_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	MAV_4989

Organism

Mycobacterium avium (strain 104) [Complete proteome] [HAMAP]

Taxonomic identifier

243243 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001015
Sites
	Metal binding	137	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	214	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A0QMH2 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 50F90CF6C133629F	FASTA	575	59,575
	10 20 30 40 50 60 MPTTDSARAA DIKQPDIKPR SRDVTDGLEK AAARGMLRAV GMGDEDFAKP QIGVASSWNE 70 80 90 100 110 120 ITPCNLSLDR LAKAVKEGVF AAGGYPLEFG TISVSDGISM GHEGMHFSLV SREVIADSVE 130 140 150 160 170 180 TVMQAERLDG SVLLAGCDKS LPGMLMAAAR LDLASVFLYA GSILPGVAKL SDGSEREVTI 190 200 210 220 230 240 IDAFEAVGAC ARGLMPREDV DAIERAICPG EGACGGMYTA NTMASAAEAL GMSLPGSAAP 250 260 270 280 290 300 PATDRRRDGF ARRSGQAVVE LLRRGITARD ILTKEAFENA IAVVMAFGGS TNAVLHLLAI 310 320 330 340 350 360 AHEADVALSL DDFSRIGSKV PHLADVKPFG RHVMTDVDHI GGVPVMMKAL LDAGLLNGDC 370 380 390 400 410 420 LTVTGATVAQ NLAAIAPPDP DGKVLRALSD PLHPTGGITI LRGSLAPEGA VVKSAGFDSD 430 440 450 460 470 480 VFEGTARVFD GERAALDALE DGTITKGDAV VIRYEGPKGG PGMREMLAIT GAIKGAGLGK 490 500 510 520 530 540 DVLLLTDGRF SGGTTGLCVG HIAPEAVDAG PIAFLRDGDR IRLDVANRVL DVLVDPAEFD 550 560 570 SRRTAFTPPP PRYKTGVLAK YVKLVGSAAI GAVCG

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 104.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000479 Genomic DNA. Translation: ABK68203.1.
RefSeq	YP_884110.1. NC_008595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0QMH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000011010; EBMYCP00000010896; EBMYCG00000011008.
GeneID	4529905.
GenomeReviews	Gene locus MAV_4989 in contig CP000479_GR.
KEGG	mav:MAV_4989.
PATRIC	17991670. VBIMycAvi38287_4902.
TIGR	MAV_4989.
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000016366.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	A0QMH2.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MAVI243243:MAV_4989-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_MYCA1

Accession

Primary (citable) accession number: A0QMH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program