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A0QME9 (TRMB_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MAV_4966
OrganismMycobacterium avium (strain 104) [Complete proteome] [HAMAP]
Taxonomic identifier243243 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288180

Regions

Region238 – 2414Substrate binding By similarity

Sites

Active site1661 By similarity
Binding site911S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1661S-adenosyl-L-methionine By similarity
Binding site1701Substrate By similarity
Binding site2021Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0QME9 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 39365AAFD659D988

FASTA25928,399
        10         20         30         40         50         60 
MGHHGQMHAQ HGVTMPADSP TGQPDRSEQR YFPATAFRTR RSTLSDAQRQ TWDRRWPELG 

        70         80         90        100        110        120 
LSVGPAEDPD RPGPPLDTDA WFGRKAPVVL EIGCGNGTST LAMAKQEPGV DVIAVEVYRR 

       130        140        150        160        170        180 
GLAQLLCAID RDNVTNIRLI RGNALDVLQR LIAPASLTGV RVFFPDPWPK ARHHKRRFLQ 

       190        200        210        220        230        240 
PGTVGLIADR LLPGGVLHVA TDHAGYAEHI ADVGAGEPRL RPADPDSPLP ISVARPTTKY 

       250 
ETKAQDAGSA VTEFIWLRR 

« Hide

References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 104.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000479 Genomic DNA. Translation: ABK66693.1.
RefSeqYP_884087.1. NC_008595.1.

3D structure databases

ProteinModelPortalA0QME9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243243.MAV_4966.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK66693; ABK66693; MAV_4966.
GeneID4525552.
KEGGmav:MAV_4966.
PATRIC17991628. VBIMycAvi38287_4881.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMATEILQDC.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycMAVI243243:GH3Y-4966-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCA1
AccessionPrimary (citable) accession number: A0QME9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 9, 2007
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways