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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium avium (strain 104)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911S-adenosyl-L-methionineUniRule annotation
Binding sitei116 – 1161S-adenosyl-L-methionineUniRule annotation
Binding sitei143 – 1431S-adenosyl-L-methionineUniRule annotation
Active sitei166 – 1661By similarity
Binding sitei166 – 1661S-adenosyl-L-methionineUniRule annotation
Binding sitei170 – 1701SubstrateUniRule annotation
Binding sitei202 – 2021SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAVI243243:GH3Y-4966-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MAV_4966
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259tRNA (guanine-N(7)-)-methyltransferasePRO_0000288180Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA0QME9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2414Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiFIHCATD.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QME9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHHGQMHAQ HGVTMPADSP TGQPDRSEQR YFPATAFRTR RSTLSDAQRQ
60 70 80 90 100
TWDRRWPELG LSVGPAEDPD RPGPPLDTDA WFGRKAPVVL EIGCGNGTST
110 120 130 140 150
LAMAKQEPGV DVIAVEVYRR GLAQLLCAID RDNVTNIRLI RGNALDVLQR
160 170 180 190 200
LIAPASLTGV RVFFPDPWPK ARHHKRRFLQ PGTVGLIADR LLPGGVLHVA
210 220 230 240 250
TDHAGYAEHI ADVGAGEPRL RPADPDSPLP ISVARPTTKY ETKAQDAGSA

VTEFIWLRR
Length:259
Mass (Da):28,399
Last modified:January 9, 2007 - v1
Checksum:i39365AAFD659D988
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66693.1.
RefSeqiWP_011726358.1. NC_008595.1.
YP_884087.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK66693; ABK66693; MAV_4966.
KEGGimav:MAV_4966.
PATRICi17991628. VBIMycAvi38287_4881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66693.1.
RefSeqiWP_011726358.1. NC_008595.1.
YP_884087.1. NC_008595.1.

3D structure databases

ProteinModelPortaliA0QME9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK66693; ABK66693; MAV_4966.
KEGGimav:MAV_4966.
PATRICi17991628. VBIMycAvi38287_4881.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiFIHCATD.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciMAVI243243:GH3Y-4966-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104.

Entry informationi

Entry nameiTRMB_MYCA1
AccessioniPrimary (citable) accession number: A0QME9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 9, 2007
Last modified: June 24, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.