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Protein

Phosphoserine phosphatase

Gene

serB

Organism
Mycobacterium avium (strain 104)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of phosphoserine (P-Ser).By similarity

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Pathwayi: L-serine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase (serA)
  2. Putative phosphoserine aminotransferase (serC)
  3. Phosphoserine phosphatase (serB)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871NucleophileBy similarity
Metal bindingi187 – 1871Magnesium
Active sitei189 – 1891Proton donorBy similarity
Metal bindingi189 – 1891Magnesium; via carbonyl oxygen
Binding sitei196 – 1961SubstrateBy similarity
Binding sitei232 – 2321SubstrateBy similarity
Binding sitei320 – 3201SubstrateBy similarity
Metal bindingi343 – 3431Magnesium
Binding sitei346 – 3461SubstrateBy similarity

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • magnesium ion binding Source: UniProtKB
  • phosphoserine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Serine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMAVI243243:GH3Y-3907-MONOMER.
UniPathwayiUPA00135; UER00198.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine phosphatase (EC:3.1.3.3)
Short name:
PSP
Short name:
PSPase
Alternative name(s):
O-phosphoserine phosphohydrolase
Gene namesi
Name:serB
Ordered Locus Names:MAV_3907
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000001574 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Phosphoserine phosphatasePRO_0000401184Add
BLAST

Proteomic databases

PRIDEiA0QJI1.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
411
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 1610Combined sources
Helixi21 – 3010Combined sources
Turni31 – 333Combined sources
Beta strandi35 – 4410Combined sources
Beta strandi47 – 5610Combined sources
Helixi58 – 614Combined sources
Helixi64 – 7613Combined sources
Beta strandi80 – 856Combined sources
Beta strandi87 – 893Combined sources
Beta strandi97 – 10610Combined sources
Helixi109 – 12113Combined sources
Beta strandi125 – 14521Combined sources
Helixi151 – 16515Combined sources
Beta strandi168 – 1736Combined sources
Turni176 – 1794Combined sources
Beta strandi183 – 1864Combined sources
Turni190 – 1923Combined sources
Beta strandi193 – 1953Combined sources
Helixi197 – 2048Combined sources
Turni208 – 2103Combined sources
Helixi214 – 2196Combined sources
Helixi225 – 23410Combined sources
Turni235 – 2384Combined sources
Helixi242 – 2487Combined sources
Helixi257 – 26610Combined sources
Beta strandi270 – 2789Combined sources
Helixi279 – 28810Combined sources
Beta strandi292 – 2976Combined sources
Beta strandi299 – 3024Combined sources
Beta strandi305 – 3106Combined sources
Helixi317 – 33115Combined sources
Helixi335 – 3373Combined sources
Beta strandi338 – 3425Combined sources
Helixi345 – 3473Combined sources
Helixi348 – 3536Combined sources
Beta strandi354 – 3618Combined sources
Helixi364 – 3674Combined sources
Beta strandi370 – 3745Combined sources
Helixi381 – 3855Combined sources
Helixi390 – 39910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P96X-ray2.05A1-411[»]
5IS2X-ray1.88A5-400[»]
5IT0X-ray1.97A5-400[»]
5IT4X-ray2.10A5-400[»]
5JJBX-ray2.31A5-400[»]
ProteinModelPortaliA0QJI1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA0QJI1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 8879ACTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni275 – 2762Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the SerB family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231114.
KOiK01079.
OMAiFTYFAKQ.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR002912. ACT_dom.
IPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QJI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSPPKVSVL ITVTGVDQPG VTATLFEVLS GHGVELLNVE QVVIRHRLTL
60 70 80 90 100
GVLVCCPADV ADGPALRHDV EAAIRKVGLD VSIERSDDVP IIREPSTHTI
110 120 130 140 150
FVLGRPITAA AFGAVAREVA ALGVNIDLIR GVSDYPVIGL ELRVSVPPGA
160 170 180 190 200
DGALRTALNR VSSEEHVDVA VEDYTLERRA KRLIVFDVDS TLVQGEVIEM
210 220 230 240 250
LAAKAGAEGQ VAAITDAAMR GELDFAQSLQ QRVATLAGLP ATVIDEVAGQ
260 270 280 290 300
LELMPGARTT LRTLRRLGYA CGVVSGGFRR IIEPLAEELM LDYVAANELE
310 320 330 340 350
IVDGTLTGRV VGPIIDRAGK ATALREFAQR AGVPMAQTVA VGDGANDIDM
360 370 380 390 400
LAAAGLGIAF NAKPALREVA DASLSHPYLD TVLFLLGVTR GEIEAADAID
410
GEVRRVEIPP E
Length:411
Mass (Da):43,339
Last modified:January 9, 2007 - v1
Checksum:i2BBB69EBBDE7D022
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66444.1.
RefSeqiWP_011725747.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK66444; ABK66444; MAV_3907.
KEGGimav:MAV_3907.
PATRICi17989500. VBIMycAvi38287_3827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66444.1.
RefSeqiWP_011725747.1. NC_008595.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P96X-ray2.05A1-411[»]
5IS2X-ray1.88A5-400[»]
5IT0X-ray1.97A5-400[»]
5IT4X-ray2.10A5-400[»]
5JJBX-ray2.31A5-400[»]
ProteinModelPortaliA0QJI1.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA0QJI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK66444; ABK66444; MAV_3907.
KEGGimav:MAV_3907.
PATRICi17989500. VBIMycAvi38287_3827.

Phylogenomic databases

HOGENOMiHOG000231114.
KOiK01079.
OMAiFTYFAKQ.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00198.
BioCyciMAVI243243:GH3Y-3907-MONOMER.

Miscellaneous databases

EvolutionaryTraceiA0QJI1.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR002912. ACT_dom.
IPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERB_MYCA1
AccessioniPrimary (citable) accession number: A0QJI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.