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Protein

Phosphoserine phosphatase

Gene

serB

Organism
Mycobacterium avium (strain 104)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of phosphoserine (P-Ser).By similarity

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Pathwayi: L-serine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase (serA)
  2. Putative phosphoserine aminotransferase (serC)
  3. Phosphoserine phosphatase (serB)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei187NucleophileBy similarity1
Metal bindingi187MagnesiumCombined sources1 Publication1
Active sitei189Proton donorBy similarity1
Metal bindingi189Magnesium; via carbonyl oxygenCombined sources1 Publication1
Binding sitei196SubstrateBy similarity1
Binding sitei232SubstrateBy similarity1
Binding sitei320SubstrateBy similarity1
Metal bindingi343MagnesiumCombined sources1 Publication1
Binding sitei346SubstrateBy similarity1

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • magnesium ion binding Source: UniProtKB
  • phosphoserine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Serine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00135; UER00198.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine phosphatase (EC:3.1.3.3)
Short name:
PSP
Short name:
PSPase
Alternative name(s):
O-phosphoserine phosphohydrolase
Gene namesi
Name:serB
Ordered Locus Names:MAV_3907
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000001574 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004011841 – 411Phosphoserine phosphataseAdd BLAST411

Proteomic databases

PRIDEiA0QJI1.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 16Combined sources10
Helixi21 – 30Combined sources10
Turni31 – 33Combined sources3
Beta strandi35 – 44Combined sources10
Beta strandi47 – 56Combined sources10
Helixi58 – 61Combined sources4
Helixi64 – 76Combined sources13
Beta strandi80 – 85Combined sources6
Beta strandi87 – 89Combined sources3
Beta strandi97 – 106Combined sources10
Helixi109 – 121Combined sources13
Beta strandi125 – 145Combined sources21
Helixi151 – 165Combined sources15
Beta strandi168 – 173Combined sources6
Turni176 – 179Combined sources4
Beta strandi183 – 186Combined sources4
Turni190 – 192Combined sources3
Beta strandi193 – 195Combined sources3
Helixi197 – 204Combined sources8
Turni208 – 210Combined sources3
Helixi214 – 219Combined sources6
Helixi225 – 234Combined sources10
Turni235 – 238Combined sources4
Helixi242 – 248Combined sources7
Helixi257 – 266Combined sources10
Beta strandi270 – 278Combined sources9
Helixi279 – 288Combined sources10
Beta strandi292 – 297Combined sources6
Beta strandi299 – 302Combined sources4
Beta strandi305 – 310Combined sources6
Helixi317 – 331Combined sources15
Helixi335 – 337Combined sources3
Beta strandi338 – 342Combined sources5
Helixi345 – 347Combined sources3
Helixi348 – 353Combined sources6
Beta strandi354 – 361Combined sources8
Helixi364 – 367Combined sources4
Beta strandi370 – 374Combined sources5
Helixi381 – 385Combined sources5
Helixi390 – 399Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P96X-ray2.05A1-411[»]
5IS2X-ray1.88A5-400[»]
5IT0X-ray1.97A5-400[»]
5IT4X-ray2.10A5-400[»]
5JJBX-ray2.31A5-400[»]
ProteinModelPortaliA0QJI1.
SMRiA0QJI1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA0QJI1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 88ACTPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni275 – 276Substrate bindingBy similarity2

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231114.
KOiK01079.
OMAiFTYFAKQ.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR002912. ACT_dom.
IPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QJI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSPPKVSVL ITVTGVDQPG VTATLFEVLS GHGVELLNVE QVVIRHRLTL
60 70 80 90 100
GVLVCCPADV ADGPALRHDV EAAIRKVGLD VSIERSDDVP IIREPSTHTI
110 120 130 140 150
FVLGRPITAA AFGAVAREVA ALGVNIDLIR GVSDYPVIGL ELRVSVPPGA
160 170 180 190 200
DGALRTALNR VSSEEHVDVA VEDYTLERRA KRLIVFDVDS TLVQGEVIEM
210 220 230 240 250
LAAKAGAEGQ VAAITDAAMR GELDFAQSLQ QRVATLAGLP ATVIDEVAGQ
260 270 280 290 300
LELMPGARTT LRTLRRLGYA CGVVSGGFRR IIEPLAEELM LDYVAANELE
310 320 330 340 350
IVDGTLTGRV VGPIIDRAGK ATALREFAQR AGVPMAQTVA VGDGANDIDM
360 370 380 390 400
LAAAGLGIAF NAKPALREVA DASLSHPYLD TVLFLLGVTR GEIEAADAID
410
GEVRRVEIPP E
Length:411
Mass (Da):43,339
Last modified:January 9, 2007 - v1
Checksum:i2BBB69EBBDE7D022
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66444.1.
RefSeqiWP_011725747.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK66444; ABK66444; MAV_3907.
KEGGimav:MAV_3907.
PATRICi17989500. VBIMycAvi38287_3827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK66444.1.
RefSeqiWP_011725747.1. NC_008595.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P96X-ray2.05A1-411[»]
5IS2X-ray1.88A5-400[»]
5IT0X-ray1.97A5-400[»]
5IT4X-ray2.10A5-400[»]
5JJBX-ray2.31A5-400[»]
ProteinModelPortaliA0QJI1.
SMRiA0QJI1.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA0QJI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK66444; ABK66444; MAV_3907.
KEGGimav:MAV_3907.
PATRICi17989500. VBIMycAvi38287_3827.

Phylogenomic databases

HOGENOMiHOG000231114.
KOiK01079.
OMAiFTYFAKQ.

Enzyme and pathway databases

UniPathwayiUPA00135; UER00198.

Miscellaneous databases

EvolutionaryTraceiA0QJI1.

Family and domain databases

Gene3Di1.10.150.210. 1 hit.
3.40.50.1000. 1 hit.
InterProiIPR002912. ACT_dom.
IPR023214. HAD-like_dom.
IPR006383. HAD-SF_hydro_IB_PSP-like.
IPR023190. Pser_Pase_dom_2.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01488. HAD-SF-IB. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSERB_MYCA1
AccessioniPrimary (citable) accession number: A0QJI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.