A0QJ14 (DXR_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	MAV_3727

Organism

Mycobacterium avium (strain 104) [Complete proteome] [HAMAP]

Taxonomic identifier

243243 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000020277
Regions
	Nucleotide binding	19 – 48	30	NADP By similarity
Sites
	Metal binding	152	1	Divalent metal cation By similarity
	Metal binding	154	1	Divalent metal cation By similarity
	Metal binding	223	1	Divalent metal cation By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QJ14 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 629AEEF2B36C8134	FASTA	407	42,315
	10 20 30 40 50 60 MTNPTSDGQP RGRLRVLVLG STGSIGTQAL QVIAANPDRF EVVGLAAGGA NLDTLLRQRA 70 80 90 100 110 120 ETGVTNVAVA DEHAARRAGD IPFCGPEAAT RLVEETEADV VLNALVGALG LRPTLAALES 130 140 150 160 170 180 GARLALANKE SLIAGGPLVL AAAAPGQIVP VDSEHSALAQ CLRGGTGDEV AKLVLTASGG 190 200 210 220 230 240 PFRGWTAEEL EHVTPEQAGA HPTWSMGPMN TLNSASLVNK GLELIETHLL FGIPYDRIEV 250 260 270 280 290 300 VVHPQSIVHS MVTFVDGSTL AQASPPDMRL PISLALGWPH RVPGAAACCD FSTASSWEFE 310 320 330 340 350 360 PLDNEVFPAV ELARHAGQAG GCMTAIYNAA NEEAAAAFLA GRVSFGAIVE TIADVLHAAD 370 380 390 400 QWAPKFSETP ANVDDVLDAQ RWARQQAQRA VAQARPATVS AKTPGVV

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 104.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000479 Genomic DNA. Translation: ABK68340.1.
RefSeq	YP_882902.1. NC_008595.1.
3D structure databases
ProteinModelPortal	A0QJ14.
SMR	A0QJ14. Positions 12-392.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QJ14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000011397; EBMYCP00000011283; EBMYCG00000011395.
GeneID	4528778.
GenomeReviews	Gene locus MAV_3727 in contig CP000479_GR.
KEGG	mav:MAV_3727.
PATRIC	17989134. VBIMycAvi38287_3647.
TIGR	MAV_3727.
Phylogenomic databases
eggNOG	COG0743.
GeneTree	EBGT00050000016490.
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	MAVI243243:MAV_3727-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_MYCA1

Accession

Primary (citable) accession number: A0QJ14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program