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A0QHJ1

- BIOB_MYCA1

UniProt

A0QHJ1 - BIOB_MYCA1

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Protein
Biotin synthase
Gene
bioB, MAV_3196
Organism
Mycobacterium avium (strain 104)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi157 – 1571Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi216 – 2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAVI243243:GH3Y-3196-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MAV_3196
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000001574: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381475Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243243.MAV_3196.

Structurei

3D structure databases

ProteinModelPortaliA0QHJ1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QHJ1-1 [UniParc]FASTAAdd to Basket

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MTQAATRPTA ETGNDEDILA VARRQVLEGG QGLSRDQVLQ VLRLPDDRLD    50
DLLALAHEVR MRWCGPEVEV EGIISLKTGG CPEDCHFCSQ SGLFASPVRS 100
ARLDIPSLVE AAKQTAKSGA TEFCIVAAVR GPDERLLAQV AAGIEAIRNE 150
VEINIACSLG MLTAEQVEQL AEMGVHRYNH NLETARSFFP NVVTTHTWEE 200
RWDTLSMVRD AGMEVCCGGI LGMGETLEQR AEFAAELAEL GPDEVPLNFL 250
NPRPGTPFGD LEVMPATEAL KSVAAFRLAL PRTMLRFAGG REITLGDLGA 300
KKGILGGINA VIVGNYLTTL GRPAESDLEL LDDLQMPLKG LNASL 345
Length:345
Mass (Da):37,200
Last modified:January 9, 2007 - v1
Checksum:iC35C84302BEC56BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000479 Genomic DNA. Translation: ABK65626.1.
RefSeqiYP_882379.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK65626; ABK65626; MAV_3196.
GeneIDi4528322.
KEGGimav:MAV_3196.
PATRICi17988085. VBIMycAvi38287_3127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000479 Genomic DNA. Translation: ABK65626.1 .
RefSeqi YP_882379.1. NC_008595.1.

3D structure databases

ProteinModelPortali A0QHJ1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243243.MAV_3196.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK65626 ; ABK65626 ; MAV_3196 .
GeneIDi 4528322.
KEGGi mav:MAV_3196.
PATRICi 17988085. VBIMycAvi38287_3127.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MAVI243243:GH3Y-3196-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104.

Entry informationi

Entry nameiBIOB_MYCA1
AccessioniPrimary (citable) accession number: A0QHJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3