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A0QHJ1 (BIOB_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MAV_3196
OrganismMycobacterium avium (strain 104) [Complete proteome] [HAMAP]
Taxonomic identifier243243 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Biotin synthase HAMAP-Rule MF_01694
PRO_0000381475

Sites

Metal binding811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1571Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2861Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0QHJ1 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: C35C84302BEC56BB

FASTA34537,200
        10         20         30         40         50         60 
MTQAATRPTA ETGNDEDILA VARRQVLEGG QGLSRDQVLQ VLRLPDDRLD DLLALAHEVR 

        70         80         90        100        110        120 
MRWCGPEVEV EGIISLKTGG CPEDCHFCSQ SGLFASPVRS ARLDIPSLVE AAKQTAKSGA 

       130        140        150        160        170        180 
TEFCIVAAVR GPDERLLAQV AAGIEAIRNE VEINIACSLG MLTAEQVEQL AEMGVHRYNH 

       190        200        210        220        230        240 
NLETARSFFP NVVTTHTWEE RWDTLSMVRD AGMEVCCGGI LGMGETLEQR AEFAAELAEL 

       250        260        270        280        290        300 
GPDEVPLNFL NPRPGTPFGD LEVMPATEAL KSVAAFRLAL PRTMLRFAGG REITLGDLGA 

       310        320        330        340 
KKGILGGINA VIVGNYLTTL GRPAESDLEL LDDLQMPLKG LNASL 

« Hide

References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 104.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000479 Genomic DNA. Translation: ABK65626.1.
RefSeqYP_882379.1. NC_008595.1.

3D structure databases

ProteinModelPortalA0QHJ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243243.MAV_3196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK65626; ABK65626; MAV_3196.
GeneID4528322.
KEGGmav:MAV_3196.
PATRIC17988085. VBIMycAvi38287_3127.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMAVI243243:GH3Y-3196-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYCA1
AccessionPrimary (citable) accession number: A0QHJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways