A0QHB6 (SYFA_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	MAV_3120

Organism

Mycobacterium avium (strain 104) [Complete proteome] [HAMAP]

Taxonomic identifier

243243 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000119399
Sites
	Metal binding	260	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QHB6 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 53EF6FBEA51203A7	FASTA	342	37,402
	10 20 30 40 50 60 MDLSETALAE AVGAARQAFA RAGDLDALAR LKTEHLGDRA PLALARQALG GVPKDQRADA 70 80 90 100 110 120 GKRVNAARAQ AQQAYDERLA ELRAERDAAV LVAERIDVTL PSTRQPVGAR HPITILAEHI 130 140 150 160 170 180 ADTFIAMGWE LAEGPEVEAE QFNFDALNFP ADHPARSEQD TFYIAPEGSR QLLRTHTSPV 190 200 210 220 230 240 QVRTLLAREL PVYVISIGRT FRTDELDATH TPVFHQVEGL AVDRGLSMAH LRGTLDAFAR 250 260 270 280 290 300 AEFGPQARTR IRPHFFPFTE PSAEVDVWFV GKKGGAGWVE WGGCGMVHPN VLRAAGIDPD 310 320 330 340 VYSGFAFGMG LERTLQFRNG IPDMRDMVEG DMRFSLPFGV GA

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 104.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000479 Genomic DNA. Translation: ABK68100.1.
RefSeq	YP_882304.1. NC_008595.1.
3D structure databases
ProteinModelPortal	A0QHB6.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QHB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000013993; EBMYCP00000013879; EBMYCG00000013991.
GeneID	4528998.
GenomeReviews	Gene locus MAV_3120 in contig CP000479_GR.
KEGG	mav:MAV_3120.
PATRIC	17987931. VBIMycAvi38287_3051.
TIGR	MAV_3120.
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000016426.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	A0QHB6.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MAVI243243:MAV_3120-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCA1

Accession

Primary (citable) accession number: A0QHB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program