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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium avium (strain 104)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei341 – 3411Proton acceptorUniRule annotation
Binding sitei341 – 3411GlyoxylateUniRule annotation
Metal bindingi439 – 4391MagnesiumUniRule annotation
Binding sitei439 – 4391GlyoxylateUniRule annotation
Metal bindingi467 – 4671MagnesiumUniRule annotation
Binding sitei548 – 5481Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei638 – 6381Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMAVI243243:GH3Y-2880-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MAV_2880
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000001574: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734Malate synthase GPRO_1000056904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243243.MAV_2880.

Structurei

3D structure databases

ProteinModelPortaliA0QGM8.
SMRiA0QGM8. Positions 5-732.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni464 – 4674Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QGM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NDEALPGTDI DPDSFWAGVD KVVTDLTPRN
60 70 80 90 100
QELLRRRDEL QAQIDKWHRQ RVIEPLDIDA YRDFLIEIGY LLPEPEDFTI
110 120 130 140 150
TTSGVDDEIT TTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDVIPE
160 170 180 190 200
TDGAEKGSSY NKVRGDKVIA YARNFLDQAV PLESGSWADA TGLSVEDGRL
210 220 230 240 250
QVATADGSVG LAEPEKFAGY TGQLGSPDWS VLLVNHGLHI EILIDPQSPV
260 270 280 290 300
GKTDRAGIKD VVLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLNKGDL
310 320 330 340 350
SEEVSKDGKT FTRVLNADRT YTTPDGQGEL TLPGRSLLFV RNVGHLMTND
360 370 380 390 400
AIVLSDGDEE KEVFEGIMDA LFTGLTAIHG LKTGEANGPL QNSRTGSIYI
410 420 430 440 450
VKPKMHGPDE VAFTCELFSR VEDVLGLPQG TLKIGIMDEE RRTTVNLKAC
460 470 480 490 500
IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN TTWIKAYEDA
510 520 530 540 550
NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP
560 570 580 590 600
TAATLHAMHY HYVDVGAVQE ELAGKKRTTI EQLLTIPLAK ELAWAPEEIR
610 620 630 640 650
EEVDNNCQSI LGYVVRWVAQ GVGCSKVPDI HDVALMEDRA TLRISSQLLA
660 670 680 690 700
NWLRHGVITE EDVRASLERM APLVDAQNAK DAAYQPMAPN FDDSLAFLAA
710 720 730
QDLILTGTQQ PNGYTEPILH RRRREVKARA AQSN
Length:734
Mass (Da):80,330
Last modified:January 9, 2007 - v1
Checksum:i8C5BA0A77D957361
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK64489.1.
RefSeqiYP_882066.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK64489; ABK64489; MAV_2880.
GeneIDi4525650.
KEGGimav:MAV_2880.
PATRICi17987453. VBIMycAvi38287_2813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK64489.1.
RefSeqiYP_882066.1. NC_008595.1.

3D structure databases

ProteinModelPortaliA0QGM8.
SMRiA0QGM8. Positions 5-732.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243243.MAV_2880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK64489; ABK64489; MAV_2880.
GeneIDi4525650.
KEGGimav:MAV_2880.
PATRICi17987453. VBIMycAvi38287_2813.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMAVI243243:GH3Y-2880-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104.

Entry informationi

Entry nameiMASZ_MYCA1
AccessioniPrimary (citable) accession number: A0QGM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.