A0QGA4 (KATG_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MAV_2753

Organism

Mycobacterium avium (strain 104) [Complete proteome] [HAMAP]

Taxonomic identifier

243243 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Catalase-peroxidase HAMAP MF_01961		PRO_0000354835
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	279	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 238		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264) By similarity
	Cross-link	238 ↔ 264		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QGA4 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 6BEDEBA89861472A	FASTA	748	81,661
	10 20 30 40 50 60 MSSDTSASRP PQPDTGTASK SESENPAIPS PHPKSNAPLT NRDWWPNQID VSRLHPHVAE 70 80 90 100 110 120 ANPLGEDFDY AEEFAKLDVE ALKADVISVM TTSQDWWPAD YGHYGGLFIR MSWHAAGTYR 130 140 150 160 170 180 IHDGRGGGGQ GMQRFAPLNS WPDNVSLDKA RRLLWPVKKK YGNKISWADL IIFAGNCALE 190 200 210 220 230 240 SMGFKTFGFA FGREDVWEPE EILWGEEDEW LGTDKRYPGT GERELAQPYG ATTMGLIYVN 250 260 270 280 290 300 PEGPEGKPDP IAAAIDIRET FGRMAMNDEE TAALIVGGHS FGKTHGAGDA DLVGPEPEAA 310 320 330 340 350 360 PIEQQGLGWK SSYGTGVGKD AITSGLEVVW TPTPTKWDNT FLETLYGYEW ELTKSPAGAW 370 380 390 400 410 420 QFTAKDGAGA GTIPDPFGGP GRAPTMLVTD ISLRESPIYR DITRRWLDHP EELADAFAKA 430 440 450 460 470 480 WYKLLHRDMG PVSRFLGPWV PEPQLWQDPV PPVDHPLVDD NDVAALKDKV LASGLSVPQL 490 500 510 520 530 540 VKTAWSAAGS YRNTDKRGGA NGGRLRLQPQ RNWEANEPSE LDKVLPVLEK IQQDFNASAS 550 560 570 580 590 600 GGKKISLADL IVLAGSAAVE KAAKDAGYEI SVHFAPGRTD ASQESTDVDS FAVLEPRADG 610 620 630 640 650 660 FRNFARPGEK APLEQLLLER AYLLGVTGPE MTVLVGGLRA LGANHGGSKH GVFTDRPGAL 670 680 690 700 710 720 TNDFFVNLLD MGTEWKASET AENVYEGHDR ATGALKWTAT ANDLVFGSNS VLRALAEVYA 730 740 QDDNQGKFVE DFVAAWVKVM NNDRFDLK

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 104.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000479 Genomic DNA. Translation: ABK67811.1.
RefSeq	YP_881942.1. NC_008595.1.
3D structure databases
ProteinModelPortal	A0QGA4.
SMR	A0QGA4. Positions 41-748.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QGA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000012069; EBMYCP00000011955; EBMYCG00000012067.
GeneID	4528744.
GenomeReviews	Gene locus MAV_2753 in contig CP000479_GR.
KEGG	mav:MAV_2753.
PATRIC	17987201. VBIMycAvi38287_2687.
TIGR	MAV_2753.
Phylogenomic databases
eggNOG	COG0376.
GeneTree	EBGT00050000017549.
HOGENOM	HBG285610.
OMA	QGKFVED.
PhylomeDB	A0QGA4.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	MAVI243243:MAV_2753-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCA1

Accession

Primary (citable) accession number: A0QGA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program