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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Mycobacterium avium (strain 104)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygenUniRule annotation1
Binding sitei203UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei211UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei405Meso-diaminopimelateUniRule annotation1
Binding sitei483Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei487Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 140ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:MAV_2331
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000001574 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000585891 – 520UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei243N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0QF47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 177UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni429 – 432Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi429 – 432Meso-diaminopimelate recognition motif4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000268118.
KOiK01928.
OMAiGRFNLYN.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QF47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVPTGLRP SAVPGVRLPA LADQVGAVMA GPDRRAAVPD VTVTGVTLRA
60 70 80 90 100
QDVLPGDLFA ALPGATTHGA RHAAEAIERG AVAVLTDAAG VAQLTGGRTT
110 120 130 140 150
PTLLHPRPRS VLGELAAAVY AHPSERLTVI GITGTSGKTT TTYLVESGLR
160 170 180 190 200
AAGRTAGLIG TVGIRIDGAD IPSALTTPEA PALQALLAAM AEQRVDTVVM
210 220 230 240 250
EVSSHALALG RVDGTRFAVG GFTNLSRDHL DFHPTMADYF EAKALLFDPN
260 270 280 290 300
SPLRAHRAVV CIDDEAGREM AARAGDAVTV SAEGQPAHWR AVEVAPLGTG
310 320 330 340 350
GQQFTVIDPA GVQHRAGIRL PGHYNVANCL VALALLDAVG VSPEQAAPGL
360 370 380 390 400
LQTRVPGRME EIDAGQDFLA LVDYAHKPGA LRAVLSSLKR PDRRLAVVFG
410 420 430 440 450
AGGERDPGKR APMGATAAEL ADLVVVTDDN PRGEDPAAIR REILAGATES
460 470 480 490 500
GCAAEVIEIG DRRAAIRHAV AWAGPGDVVL VAGKGHETGQ RTGEHTRPFD
510 520
DRVELAEALR EAVGPRKAQG
Length:520
Mass (Da):53,765
Last modified:January 9, 2007 - v1
Checksum:iC22B8555632D09C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK64811.1.
RefSeqiWP_009976555.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK64811; ABK64811; MAV_2331.
KEGGimav:MAV_2331.
PATRICi17986364. VBIMycAvi38287_2270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000479 Genomic DNA. Translation: ABK64811.1.
RefSeqiWP_009976555.1. NC_008595.1.

3D structure databases

ProteinModelPortaliA0QF47.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK64811; ABK64811; MAV_2331.
KEGGimav:MAV_2331.
PATRICi17986364. VBIMycAvi38287_2270.

Phylogenomic databases

HOGENOMiHOG000268118.
KOiK01928.
OMAiGRFNLYN.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURE_MYCA1
AccessioniPrimary (citable) accession number: A0QF47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.