A0QF44 (RSMH_MYCA1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	MAV_2328

Organism

Mycobacterium avium (strain 104) [Complete proteome] [HAMAP]

Taxonomic identifier

243243 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386985
Regions
	Region	46 – 48	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	65	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QF44 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: FF5372BA5FD82584	FASTA	335	36,471
	10 20 30 40 50 60 MVDDSPDFGH VPVLLERCVE LLTPALTRRH PDGSGAVLLD ATLGAGGHAE RFLTDLPGLR 70 80 90 100 110 120 LIALDRDPSA LEIARERLAR FADRITLVHT RYDGIAAALT ESGYAATESV DGILFDLGVS 130 140 150 160 170 180 SMQLDRPERG FAYAQDAPLD MRMDPGSPLT AADILNTYDE AELADILHRY GEERFARRIA 190 200 210 220 230 240 AQIVRRRAKE PFTSTADLVS LLYQAIPAPA RRTGGHPAKR TFQALRIAVN DELDTLRCAL 250 260 270 280 290 300 PAALDALAVD GRIVVLAYQS LEDRIVKRLF AQAVASRTPV DLPVELPGHE PRFRALTHGA 310 320 330 GRADAAEIER NPRSAAVRLR ALQRTQAPQA TGKGD

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 104.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000479 Genomic DNA. Translation: ABK66998.1.
RefSeq	YP_881532.1. NC_008595.1.
3D structure databases
ProteinModelPortal	A0QF44.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QF44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000013206; EBMYCP00000013092; EBMYCG00000013204.
GeneID	4527633.
GenomeReviews	Gene locus MAV_2328 in contig CP000479_GR.
KEGG	mav:MAV_2328.
PATRIC	17986358. VBIMycAvi38287_2267.
TIGR	MAV_2328.
Phylogenomic databases
eggNOG	COG0275.
GeneTree	EBGT00050000016736.
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	MAVI243243:MAV_2328-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_MYCA1

Accession

Primary (citable) accession number: A0QF44

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program