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A0QEY6

- LIPA_MYCA1

UniProt

A0QEY6 - LIPA_MYCA1

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Protein

Lipoyl synthase

Gene
lipA, MAV_2270
Organism
Mycobacterium avium (strain 104)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMAVI243243:GH3Y-2270-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:MAV_2270
OrganismiMycobacterium avium (strain 104)
Taxonomic identifieri243243 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000001574: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Lipoyl synthaseUniRule annotationPRO_1000012234Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243243.MAV_2270.

Structurei

3D structure databases

ProteinModelPortaliA0QEY6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiEWLRRPI.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QEY6-1 [UniParc]FASTAAdd to Basket

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MTVAPEGRKL LRLEVRNADT PIERKPPWIR VRARMGPEYT ELKSLVRREG    50
LHTVCEEAGC PNIFECWEDR EATFLIGGDQ CTRRCDFCQI DTGKPAELDR 100
DEPRRVADSV RTMGLRYATV TGVARDDLPD GGAWLYAETV RAIKELNPST 150
GVELLIPDFN GRPDRLAEVF GSRPEVLAHN VETVPRIFKR IRPAFTYRRS 200
LDVLTAAREA GLVTKSNLIL GLGETPDEVR TALADLRGAG CDIITITQYL 250
RPSARHHPVE RWVKPEEFVE FARHAEELGF SGVLAGPLVR SSYRAGRLYR 300
QAARARA 307
Length:307
Mass (Da):34,532
Last modified:January 9, 2007 - v1
Checksum:iB3CB822B73F3291C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000479 Genomic DNA. Translation: ABK66875.1.
RefSeqiWP_011724695.1. NC_008595.1.
YP_881474.1. NC_008595.1.

Genome annotation databases

EnsemblBacteriaiABK66875; ABK66875; MAV_2270.
GeneIDi4527880.
KEGGimav:MAV_2270.
PATRICi17986244. VBIMycAvi38287_2210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000479 Genomic DNA. Translation: ABK66875.1 .
RefSeqi WP_011724695.1. NC_008595.1.
YP_881474.1. NC_008595.1.

3D structure databases

ProteinModelPortali A0QEY6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243243.MAV_2270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK66875 ; ABK66875 ; MAV_2270 .
GeneIDi 4527880.
KEGGi mav:MAV_2270.
PATRICi 17986244. VBIMycAvi38287_2210.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi EWLRRPI.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci MAVI243243:GH3Y-2270-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104.

Entry informationi

Entry nameiLIPA_MYCA1
AccessioniPrimary (citable) accession number: A0QEY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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