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A0Q7W6

- RNC_FRATN

UniProt

A0Q7W6 - RNC_FRATN

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Protein

Ribonuclease 3

Gene
rnc, FTN_1463
Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity.UniRule annotation
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA Inferred. Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370 (1 Publication).

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Magnesium By similarity
Active sitei44 – 441 Reviewed prediction
Metal bindingi111 – 1111Magnesium By similarity
Active sitei114 – 1141 By similarity
Metal bindingi114 – 1141Magnesium By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciFNOV401614:GC4M-1462-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:FTN_1463
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000000762: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Ribonuclease 3UniRule annotationPRO_1000075757Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi401614.FTN_1463.

Structurei

3D structure databases

ProteinModelPortaliA0Q7W6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 125121RNase IIIAdd
BLAST
Domaini153 – 22371DRBMAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246809.
KOiK03685.
OMAiAQKDPKT.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0Q7W6-1 [UniParc]FASTAAdd to Basket

« Hide

MVPEYSRFYN ILGYNFKDYT LLIRALTHRS KTKKNYERLE FLGDSVLSFV    50
IAEVLYKQFT DLAEGKLSQL RSKLVKGTTL AQLASSLKMD EYIILGASEQ 100
GGHKREKILE DVFEAVIGAI YLDSDFATVK KVILKWYQPI ISSINLDTIK 150
VKDSKSKLQE ILLQNALSLP EYSIETIDGK DHEQQFTVVA MSKDLNLRVK 200
AQGTSRKKAE QKAAEKMIEM LSQQGLHEKK 230
Length:230
Mass (Da):26,229
Last modified:January 9, 2007 - v1
Checksum:iFC742D0460DF4859
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000439 Genomic DNA. Translation: ABK90331.1.
RefSeqiYP_899085.1. NC_008601.1.

Genome annotation databases

EnsemblBacteriaiABK90331; ABK90331; FTN_1463.
GeneIDi4548558.
KEGGiftn:FTN_1463.
PATRICi17936089. VBIFraNov128299_1496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000439 Genomic DNA. Translation: ABK90331.1 .
RefSeqi YP_899085.1. NC_008601.1.

3D structure databases

ProteinModelPortali A0Q7W6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 401614.FTN_1463.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK90331 ; ABK90331 ; FTN_1463 .
GeneIDi 4548558.
KEGGi ftn:FTN_1463.
PATRICi 17936089. VBIFraNov128299_1496.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246809.
KOi K03685.
OMAi AQKDPKT.
OrthoDBi EOG6T1WVS.

Enzyme and pathway databases

BioCyci FNOV401614:GC4M-1462-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: U112.
  2. "Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems."
    Fonfara I., Le Rhun A., Chylinski K., Makarova K.S., Lecrivain A.L., Bzdrenga J., Koonin E.V., Charpentier E.
    Nucleic Acids Res. 42:2577-2590(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: U112.

Entry informationi

Entry nameiRNC_FRATN
AccessioniPrimary (citable) accession number: A0Q7W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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