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Protein

Ribonuclease 3

Gene

rnc

Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity).By similarity
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (Probable). Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370 (PubMed:24270795).Curated1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40MagnesiumUniRule annotation1
Active sitei44UniRule annotation1
Metal bindingi111MagnesiumUniRule annotation1
Active sitei114UniRule annotation1
Metal bindingi114MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:FTN_1463
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000000762 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000757571 – 230Ribonuclease 3Add BLAST230

Proteomic databases

PRIDEiA0Q7W6

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0Q7W6
SMRiA0Q7W6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 125RNase IIIUniRule annotationAdd BLAST121
Domaini153 – 223DRBMUniRule annotationAdd BLAST71

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246809
KOiK03685
OMAiLTHKSCK

Family and domain databases

CDDicd00593 RIBOc, 1 hit
Gene3Di1.10.1520.10, 1 hit
HAMAPiMF_00104 RNase_III, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR011907 RNase_III
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF14622 Ribonucleas_3_3, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00535 RIBOc, 1 hit
SUPFAMiSSF69065 SSF69065, 1 hit
TIGRFAMsiTIGR02191 RNaseIII, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0Q7W6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPEYSRFYN ILGYNFKDYT LLIRALTHRS KTKKNYERLE FLGDSVLSFV
60 70 80 90 100
IAEVLYKQFT DLAEGKLSQL RSKLVKGTTL AQLASSLKMD EYIILGASEQ
110 120 130 140 150
GGHKREKILE DVFEAVIGAI YLDSDFATVK KVILKWYQPI ISSINLDTIK
160 170 180 190 200
VKDSKSKLQE ILLQNALSLP EYSIETIDGK DHEQQFTVVA MSKDLNLRVK
210 220 230
AQGTSRKKAE QKAAEKMIEM LSQQGLHEKK
Length:230
Mass (Da):26,229
Last modified:January 9, 2007 - v1
Checksum:iFC742D0460DF4859
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000439 Genomic DNA Translation: ABK90331.1
RefSeqiWP_003034807.1, NZ_CP009633.1

Genome annotation databases

EnsemblBacteriaiABK90331; ABK90331; FTN_1463
KEGGiftn:FTN_1463
ftx:AW25_538

Similar proteinsi

Entry informationi

Entry nameiRNC_FRATN
AccessioniPrimary (citable) accession number: A0Q7W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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