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Protein

CRISPR-associated endonuclease Cpf1

Gene

cpf1

Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a single-RNA guided endonuclease that is also capable of guiding crRNA processing; correct processing of pre-crRNA requires only this protein and the CRISPR locus (PubMed:26422227). When this protein is expressed in E.coli it prevents plasmids homologous to the first CRISPR spacer from transforming, showing it is responsible for plasmid immunity (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 5-base 5' overhangs 18 and 23 bases downstream of the PAM (protospacer adjacent motif) on the non-targeted and targeted strands respectively (PubMed:26422227).1 Publication

Miscellaneous

Part of a type V-A CRISPR-Cas system.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei917For RuvC domain1 Publication1
Active sitei1006For RuvC domain1 Publication1
Active sitei1255For Nuc domain1 Publication1
Active sitei1257For RuvC domain1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding
Biological processAntiviral defense
LigandMagnesium

Enzyme and pathway databases

BioCyciFTUL401614:G12WZ-1387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated endonuclease Cpf11 Publication (EC:3.1.-.-)
Alternative name(s):
FnCpf11 Publication
Gene namesi
Name:cpf11 Publication
Ordered Locus Names:FTN_1397
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000000762 Componenti: Chromosome

Pathology & Biotechi

Biotechnological usei

Despite being appropriately expressed in a human cell line, it does not mediate human genome editing.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi917D → A: Loss of dsDNA cleavage. 1 Publication1
Mutagenesisi1006E → A: Loss of dsDNA cleavage. 1 Publication1
Mutagenesisi1255D → A: Slight decrease in dsDNA cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004349021 – 1300CRISPR-associated endonuclease Cpf1Add BLAST1300

Interactioni

Subunit structurei

Might be a homodimer.1 Publication

Structurei

Secondary structure

11300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 6Combined sources4
Beta strandi13 – 23Combined sources11
Helixi27 – 34Combined sources8
Helixi36 – 68Combined sources33
Helixi73 – 86Combined sources14
Helixi92 – 114Combined sources23
Helixi117 – 120Combined sources4
Beta strandi122 – 124Combined sources3
Helixi125 – 127Combined sources3
Beta strandi132 – 134Combined sources3
Helixi137 – 147Combined sources11
Helixi153 – 155Combined sources3
Helixi162 – 171Combined sources10
Turni172 – 174Combined sources3
Helixi176 – 179Combined sources4
Helixi180 – 190Combined sources11
Beta strandi192 – 194Combined sources3
Beta strandi196 – 198Combined sources3
Helixi199 – 204Combined sources6
Helixi207 – 224Combined sources18
Helixi226 – 228Combined sources3
Helixi231 – 237Combined sources7
Turni238 – 242Combined sources5
Beta strandi243 – 245Combined sources3
Turni248 – 251Combined sources4
Beta strandi252 – 257Combined sources6
Helixi260 – 263Combined sources4
Helixi267 – 271Combined sources5
Beta strandi272 – 274Combined sources3
Helixi275 – 286Combined sources12
Beta strandi291 – 293Combined sources3
Beta strandi294 – 297Combined sources4
Helixi300 – 311Combined sources12
Helixi314 – 319Combined sources6
Helixi345 – 361Combined sources17
Helixi370 – 382Combined sources13
Helixi388 – 390Combined sources3
Beta strandi392 – 394Combined sources3
Helixi397 – 406Combined sources10
Helixi412 – 422Combined sources11
Helixi438 – 442Combined sources5
Turni445 – 447Combined sources3
Beta strandi449 – 452Combined sources4
Helixi453 – 465Combined sources13
Beta strandi469 – 471Combined sources3
Helixi475 – 484Combined sources10
Helixi487 – 506Combined sources20
Helixi513 – 515Combined sources3
Helixi517 – 519Combined sources3
Helixi520 – 541Combined sources22
Helixi559 – 572Combined sources14
Helixi575 – 586Combined sources12
Beta strandi595 – 597Combined sources3
Turni603 – 606Combined sources4
Helixi611 – 613Combined sources3
Helixi614 – 617Combined sources4
Beta strandi619 – 624Combined sources6
Beta strandi627 – 633Combined sources7
Helixi635 – 637Combined sources3
Turni638 – 641Combined sources4
Helixi643 – 648Combined sources6
Beta strandi650 – 661Combined sources12
Helixi665 – 673Combined sources9
Turni676 – 678Combined sources3
Helixi679 – 682Combined sources4
Helixi686 – 694Combined sources9
Turni695 – 697Combined sources3
Beta strandi706 – 708Combined sources3
Helixi714 – 730Combined sources17
Helixi734 – 737Combined sources4
Helixi744 – 746Combined sources3
Helixi750 – 760Combined sources11
Beta strandi761 – 769Combined sources9
Helixi771 – 779Combined sources9
Beta strandi782 – 789Combined sources8
Helixi791 – 793Combined sources3
Helixi803 – 812Combined sources10
Helixi814 – 818Combined sources5
Beta strandi821 – 824Combined sources4
Beta strandi829 – 833Combined sources5
Beta strandi852 – 856Combined sources5
Beta strandi857 – 861Combined sources5
Helixi871 – 874Combined sources4
Beta strandi877 – 887Combined sources11
Helixi896 – 906Combined sources11
Helixi908 – 910Combined sources3
Beta strandi912 – 917Combined sources6
Beta strandi923 – 929Combined sources7
Beta strandi935 – 944Combined sources10
Beta strandi950 – 952Combined sources3
Helixi953 – 968Combined sources16
Turni969 – 971Combined sources3
Helixi977 – 999Combined sources23
Beta strandi1001 – 1006Combined sources6
Beta strandi1008 – 1011Combined sources4
Helixi1019 – 1036Combined sources18
Beta strandi1041 – 1043Combined sources3
Beta strandi1045 – 1048Combined sources4
Beta strandi1050 – 1053Combined sources4
Helixi1065 – 1067Combined sources3
Beta strandi1069 – 1071Combined sources3
Beta strandi1074 – 1077Combined sources4
Beta strandi1083 – 1085Combined sources3
Turni1087 – 1089Combined sources3
Helixi1102 – 1110Combined sources9
Beta strandi1112 – 1118Combined sources7
Turni1119 – 1122Combined sources4
Beta strandi1123 – 1129Combined sources7
Helixi1130 – 1132Combined sources3
Beta strandi1141 – 1145Combined sources5
Beta strandi1150 – 1153Combined sources4
Beta strandi1165 – 1168Combined sources4
Helixi1170 – 1180Combined sources11
Helixi1192 – 1197Combined sources6
Helixi1201 – 1214Combined sources14
Beta strandi1218 – 1221Combined sources4
Turni1222 – 1225Combined sources4
Beta strandi1228 – 1230Combined sources3
Turni1236 – 1238Combined sources3
Beta strandi1242 – 1245Combined sources4
Helixi1254 – 1275Combined sources22
Beta strandi1278 – 1280Combined sources3
Helixi1288 – 1297Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MGAX-ray3.00A1-1300[»]
5NFVX-ray2.50A2-1300[»]
5NG6X-ray3.34A/C/E/G2-1300[»]
ProteinModelPortaliA0Q7Q2.
SMRiA0Q7Q2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000066230.
OMAiYLFQIYN.

Family and domain databases

InterProiView protein in InterPro
IPR027620. Cas_Cpf1.
TIGRFAMsiTIGR04330. cas_Cpf1. 1 hit.

Sequencei

Sequence statusi: Complete.

A0Q7Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIYQEFVNK YSLSKTLRFE LIPQGKTLEN IKARGLILDD EKRAKDYKKA
60 70 80 90 100
KQIIDKYHQF FIEEILSSVC ISEDLLQNYS DVYFKLKKSD DDNLQKDFKS
110 120 130 140 150
AKDTIKKQIS EYIKDSEKFK NLFNQNLIDA KKGQESDLIL WLKQSKDNGI
160 170 180 190 200
ELFKANSDIT DIDEALEIIK SFKGWTTYFK GFHENRKNVY SSNDIPTSII
210 220 230 240 250
YRIVDDNLPK FLENKAKYES LKDKAPEAIN YEQIKKDLAE ELTFDIDYKT
260 270 280 290 300
SEVNQRVFSL DEVFEIANFN NYLNQSGITK FNTIIGGKFV NGENTKRKGI
310 320 330 340 350
NEYINLYSQQ INDKTLKKYK MSVLFKQILS DTESKSFVID KLEDDSDVVT
360 370 380 390 400
TMQSFYEQIA AFKTVEEKSI KETLSLLFDD LKAQKLDLSK IYFKNDKSLT
410 420 430 440 450
DLSQQVFDDY SVIGTAVLEY ITQQIAPKNL DNPSKKEQEL IAKKTEKAKY
460 470 480 490 500
LSLETIKLAL EEFNKHRDID KQCRFEEILA NFAAIPMIFD EIAQNKDNLA
510 520 530 540 550
QISIKYQNQG KKDLLQASAE DDVKAIKDLL DQTNNLLHKL KIFHISQSED
560 570 580 590 600
KANILDKDEH FYLVFEECYF ELANIVPLYN KIRNYITQKP YSDEKFKLNF
610 620 630 640 650
ENSTLANGWD KNKEPDNTAI LFIKDDKYYL GVMNKKNNKI FDDKAIKENK
660 670 680 690 700
GEGYKKIVYK LLPGANKMLP KVFFSAKSIK FYNPSEDILR IRNHSTHTKN
710 720 730 740 750
GSPQKGYEKF EFNIEDCRKF IDFYKQSISK HPEWKDFGFR FSDTQRYNSI
760 770 780 790 800
DEFYREVENQ GYKLTFENIS ESYIDSVVNQ GKLYLFQIYN KDFSAYSKGR
810 820 830 840 850
PNLHTLYWKA LFDERNLQDV VYKLNGEAEL FYRKQSIPKK ITHPAKEAIA
860 870 880 890 900
NKNKDNPKKE SVFEYDLIKD KRFTEDKFFF HCPITINFKS SGANKFNDEI
910 920 930 940 950
NLLLKEKAND VHILSIDRGE RHLAYYTLVD GKGNIIKQDT FNIIGNDRMK
960 970 980 990 1000
TNYHDKLAAI EKDRDSARKD WKKINNIKEM KEGYLSQVVH EIAKLVIEYN
1010 1020 1030 1040 1050
AIVVFEDLNF GFKRGRFKVE KQVYQKLEKM LIEKLNYLVF KDNEFDKTGG
1060 1070 1080 1090 1100
VLRAYQLTAP FETFKKMGKQ TGIIYYVPAG FTSKICPVTG FVNQLYPKYE
1110 1120 1130 1140 1150
SVSKSQEFFS KFDKICYNLD KGYFEFSFDY KNFGDKAAKG KWTIASFGSR
1160 1170 1180 1190 1200
LINFRNSDKN HNWDTREVYP TKELEKLLKD YSIEYGHGEC IKAAICGESD
1210 1220 1230 1240 1250
KKFFAKLTSV LNTILQMRNS KTGTELDYLI SPVADVNGNF FDSRQAPKNM
1260 1270 1280 1290 1300
PQDADANGAY HIGLKGLMLL GRIKNNQEGK KLNLVIKNEE YFEFVQNRNN
Length:1,300
Mass (Da):151,915
Last modified:January 9, 2007 - v1
Checksum:i601E903DE68C80DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000439 Genomic DNA. Translation: ABK90267.1.
RefSeqiWP_003040289.1. NZ_CP009633.1.

Genome annotation databases

EnsemblBacteriaiABK90267; ABK90267; FTN_1397.
KEGGiftn:FTN_1397.
ftx:AW25_605.

Similar proteinsi

Entry informationi

Entry nameiCPF1_FRATN
AccessioniPrimary (citable) accession number: A0Q7Q2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: January 9, 2007
Last modified: October 25, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families