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Protein

CRISPR-associated endonuclease Cpf1

Gene

cpf1

Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein is a single-RNA guided endonuclease that is also capable of guiding crRNA processing; correct processing of pre-crRNA requires only this protein and the CRISPR locus (PubMed:26422227). When this protein is expressed in E.coli it prevents plasmids homologous to the first CRISPR spacer from transforming, showing it is responsible for plasmid immunity (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 5-base 5' overhangs 18 and 23 bases downstream of the PAM (protospacer adjacent motif) on the non-targeted and targeted strands respectively (PubMed:26422227).1 Publication

Miscellaneous

Part of a type V-A CRISPR-Cas system.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei917For RuvC domain1 Publication1
Active sitei1006For RuvC domain1 Publication1
Active sitei1255For Nuc domain1 Publication1
Active sitei1257For RuvC domain1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease, RNA-binding
Biological processAntiviral defense
LigandMagnesium

Enzyme and pathway databases

BioCyciFTUL401614:G12WZ-1387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated endonuclease Cpf11 Publication (EC:3.1.-.-)
Alternative name(s):
FnCpf11 Publication
Gene namesi
Name:cpf11 Publication
Ordered Locus Names:FTN_1397
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000000762 Componenti: Chromosome

Pathology & Biotechi

Biotechnological usei

Despite being appropriately expressed in a human cell line, it does not mediate human genome editing.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi917D → A: Loss of dsDNA cleavage. 1 Publication1
Mutagenesisi1006E → A: Loss of dsDNA cleavage. 1 Publication1
Mutagenesisi1255D → A: Slight decrease in dsDNA cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004349021 – 1300CRISPR-associated endonuclease Cpf1Add BLAST1300

Interactioni

Subunit structurei

Might be a homodimer.1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MGAX-ray3.00A1-1300[»]
5NFVX-ray2.50A2-1300[»]
5NG6X-ray3.34A/C/E/G2-1300[»]
ProteinModelPortaliA0Q7Q2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000066230.
OMAiYLFQIYN.

Family and domain databases

InterProiView protein in InterPro
IPR027620. Cas_Cpf1.
TIGRFAMsiTIGR04330. cas_Cpf1. 1 hit.

Sequencei

Sequence statusi: Complete.

A0Q7Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIYQEFVNK YSLSKTLRFE LIPQGKTLEN IKARGLILDD EKRAKDYKKA
60 70 80 90 100
KQIIDKYHQF FIEEILSSVC ISEDLLQNYS DVYFKLKKSD DDNLQKDFKS
110 120 130 140 150
AKDTIKKQIS EYIKDSEKFK NLFNQNLIDA KKGQESDLIL WLKQSKDNGI
160 170 180 190 200
ELFKANSDIT DIDEALEIIK SFKGWTTYFK GFHENRKNVY SSNDIPTSII
210 220 230 240 250
YRIVDDNLPK FLENKAKYES LKDKAPEAIN YEQIKKDLAE ELTFDIDYKT
260 270 280 290 300
SEVNQRVFSL DEVFEIANFN NYLNQSGITK FNTIIGGKFV NGENTKRKGI
310 320 330 340 350
NEYINLYSQQ INDKTLKKYK MSVLFKQILS DTESKSFVID KLEDDSDVVT
360 370 380 390 400
TMQSFYEQIA AFKTVEEKSI KETLSLLFDD LKAQKLDLSK IYFKNDKSLT
410 420 430 440 450
DLSQQVFDDY SVIGTAVLEY ITQQIAPKNL DNPSKKEQEL IAKKTEKAKY
460 470 480 490 500
LSLETIKLAL EEFNKHRDID KQCRFEEILA NFAAIPMIFD EIAQNKDNLA
510 520 530 540 550
QISIKYQNQG KKDLLQASAE DDVKAIKDLL DQTNNLLHKL KIFHISQSED
560 570 580 590 600
KANILDKDEH FYLVFEECYF ELANIVPLYN KIRNYITQKP YSDEKFKLNF
610 620 630 640 650
ENSTLANGWD KNKEPDNTAI LFIKDDKYYL GVMNKKNNKI FDDKAIKENK
660 670 680 690 700
GEGYKKIVYK LLPGANKMLP KVFFSAKSIK FYNPSEDILR IRNHSTHTKN
710 720 730 740 750
GSPQKGYEKF EFNIEDCRKF IDFYKQSISK HPEWKDFGFR FSDTQRYNSI
760 770 780 790 800
DEFYREVENQ GYKLTFENIS ESYIDSVVNQ GKLYLFQIYN KDFSAYSKGR
810 820 830 840 850
PNLHTLYWKA LFDERNLQDV VYKLNGEAEL FYRKQSIPKK ITHPAKEAIA
860 870 880 890 900
NKNKDNPKKE SVFEYDLIKD KRFTEDKFFF HCPITINFKS SGANKFNDEI
910 920 930 940 950
NLLLKEKAND VHILSIDRGE RHLAYYTLVD GKGNIIKQDT FNIIGNDRMK
960 970 980 990 1000
TNYHDKLAAI EKDRDSARKD WKKINNIKEM KEGYLSQVVH EIAKLVIEYN
1010 1020 1030 1040 1050
AIVVFEDLNF GFKRGRFKVE KQVYQKLEKM LIEKLNYLVF KDNEFDKTGG
1060 1070 1080 1090 1100
VLRAYQLTAP FETFKKMGKQ TGIIYYVPAG FTSKICPVTG FVNQLYPKYE
1110 1120 1130 1140 1150
SVSKSQEFFS KFDKICYNLD KGYFEFSFDY KNFGDKAAKG KWTIASFGSR
1160 1170 1180 1190 1200
LINFRNSDKN HNWDTREVYP TKELEKLLKD YSIEYGHGEC IKAAICGESD
1210 1220 1230 1240 1250
KKFFAKLTSV LNTILQMRNS KTGTELDYLI SPVADVNGNF FDSRQAPKNM
1260 1270 1280 1290 1300
PQDADANGAY HIGLKGLMLL GRIKNNQEGK KLNLVIKNEE YFEFVQNRNN
Length:1,300
Mass (Da):151,915
Last modified:January 9, 2007 - v1
Checksum:i601E903DE68C80DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000439 Genomic DNA. Translation: ABK90267.1.
RefSeqiWP_003040289.1. NZ_CP009633.1.

Genome annotation databases

EnsemblBacteriaiABK90267; ABK90267; FTN_1397.
KEGGiftn:FTN_1397.
ftx:AW25_605.

Similar proteinsi

Entry informationi

Entry nameiCPF1_FRATN
AccessioniPrimary (citable) accession number: A0Q7Q2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: January 9, 2007
Last modified: August 30, 2017
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families