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Protein

Thymidylate synthase

Gene

thyA

Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei156NucleophileUniRule annotation1
Binding sitei1795,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei187dUMPUniRule annotation1
Binding sitei2735,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 124dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi176 – 179dUMPUniRule annotation4
Nucleotide bindingi217 – 219dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:FTN_1248
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
Proteomesi
  • UP000000762 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006001 – 274Thymidylate synthaseAdd BLAST274

Proteomic databases

PRIDEiA0Q7B4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0Q7B4
SMRiA0Q7B4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits

Sequencei

Sequence statusi: Complete.

A0Q7B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYLNFLKY IKENGVLKGD RTGTGTRSIF GYQMRFDLQK GFPLVTTKKI
60 70 80 90 100
HIPSVVHELL WFLSGSTNIK YLNDNNVRIW NEWATVDGEL GPIYGKQWRD
110 120 130 140 150
FNGQGIDQIA DVIQMLKTNP NSRRILVSAW NPCVVPSEKI SPQENVVKGN
160 170 180 190 200
SALPPCHAMF QFYVANNKLS CMLTQRSADA FLGVPFNIAS YSLLTHMVAQ
210 220 230 240 250
QCNLDVGEFI WSGGDCHIYN NHIEQVNEQL SREPLALPTL KILRKPNSIF
260 270
DYKYEDFEFE NYNHHPAIKA KISV
Length:274
Mass (Da):31,376
Last modified:January 9, 2007 - v1
Checksum:i6A1B81E97C240C9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000439 Genomic DNA Translation: ABK90129.1
RefSeqiWP_003025777.1, NZ_CP009633.1

Genome annotation databases

EnsemblBacteriaiABK90129; ABK90129; FTN_1248
KEGGiftn:FTN_1248
ftx:AW25_758

Similar proteinsi

Entry informationi

Entry nameiTYSY_FRATN
AccessioniPrimary (citable) accession number: A0Q7B4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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