A0Q7B4 (TYSY_FRATN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	FTN_1248

Organism

Francisella tularensis subsp. novicida (strain U112) [Complete proteome] [HAMAP]

Taxonomic identifier

401614 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella novicida

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Thymidylate synthase HAMAP-Rule MF_00008		PRO_1000000600
Sites
	Active site	156	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0Q7B4 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 6A1B81E97C240C9A	FASTA	274	31,376
	10 20 30 40 50 60 MREYLNFLKY IKENGVLKGD RTGTGTRSIF GYQMRFDLQK GFPLVTTKKI HIPSVVHELL 70 80 90 100 110 120 WFLSGSTNIK YLNDNNVRIW NEWATVDGEL GPIYGKQWRD FNGQGIDQIA DVIQMLKTNP 130 140 150 160 170 180 NSRRILVSAW NPCVVPSEKI SPQENVVKGN SALPPCHAMF QFYVANNKLS CMLTQRSADA 190 200 210 220 230 240 FLGVPFNIAS YSLLTHMVAQ QCNLDVGEFI WSGGDCHIYN NHIEQVNEQL SREPLALPTL 250 260 270 KILRKPNSIF DYKYEDFEFE NYNHHPAIKA KISV

References

[1]

"Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strains." Rohmer L., Fong C., Abmayr S., Wasnick M., Larson Freeman T.J., Radey M., Guina T., Svensson K., Hayden H.S., Jacobs M., Gallagher L.A., Manoil C., Ernst R.K., Drees B., Buckley D., Haugen E., Bovee D., Zhou Y. , Chang J., Levy R., Lim R., Gillett W., Guenthener D., Kang A., Shaffer S.A., Taylor G., Chen J., Gallis B., D'Argenio D.A., Forsman M., Olson M.V., Goodlett D.R., Kaul R., Miller S.I., Brittnacher M.J. Genome Biol. 8:R102.1-R102.16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U112.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000439 Genomic DNA. Translation: ABK90129.1.
RefSeq	YP_898883.1. NC_008601.1.
3D structure databases
ProteinModelPortal	A0Q7B4.
SMR	A0Q7B4. Positions 2-274.
ModBase	Search...
Protein-protein interaction databases
STRING	401614.FTN_1248.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK90129; ABK90129; FTN_1248.
GeneID	4548047.
KEGG	ftn:FTN_1248.
PATRIC	17935647. VBIFraNov128299_1285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_FRATN

Accession

Primary (citable) accession number: A0Q7B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program