A0Q6K3 (MDH_FRATN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	FTN_0980

Organism

Francisella tularensis subsp. novicida (strain U112) [Complete proteome] [HAMAP]

Taxonomic identifier

401614 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella novicida

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_1000026473
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0Q6K3 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: FA50D9450B983FF5	FASTA	319	34,047
	10 20 30 40 50 60 MARKKIALVG AGNIGGTLAH LALLKQLGDV VLFDIAQGMP NGKALDLLQT CPIEGVDFKV 70 80 90 100 110 120 RGTNDYKDLE NSDVVIVTAG VPRKPGMSRD DLLGINIKVM QTVGEGIKHN CPNAFVICIT 130 140 150 160 170 180 NPLDIMVNML QKFSGVPDNK IVGMAGVLDS ARFRTFLADE LNVSVQQVQA YVMGGHGDTM 190 200 210 220 230 240 VPLTKMSNVA GVSLEQLVKE GKLKQERLDA IVSRTRSGGG EIVALLKTGS AYYAPAAAGI 250 260 270 280 290 300 QMAESFLKDK KMILPCAAKV KAGMYGLDED LFVGVPTEIS ANGVRPIEVE ISDKEREQLQ 310 VSINAVKDLN KAAAEILAK

References

[1]

"Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strains." Rohmer L., Fong C., Abmayr S., Wasnick M., Larson Freeman T.J., Radey M., Guina T., Svensson K., Hayden H.S., Jacobs M., Gallagher L.A., Manoil C., Ernst R.K., Drees B., Buckley D., Haugen E., Bovee D., Zhou Y. , Chang J., Levy R., Lim R., Gillett W., Guenthener D., Kang A., Shaffer S.A., Taylor G., Chen J., Gallis B., D'Argenio D.A., Forsman M., Olson M.V., Goodlett D.R., Kaul R., Miller S.I., Brittnacher M.J. Genome Biol. 8:R102.1-R102.16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U112.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000439 Genomic DNA. Translation: ABK89868.1.
RefSeq	YP_898622.1. NC_008601.1.
3D structure databases
ProteinModelPortal	A0Q6K3.
ModBase	Search...
Protein-protein interaction databases
STRING	401614.FTN_0980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK89868; ABK89868; FTN_0980.
GeneID	4547405.
KEGG	ftn:FTN_0980.
PATRIC	17935092. VBIFraNov128299_1013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DANIVEC.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_FRATN

Accession

Primary (citable) accession number: A0Q6K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program