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A0Q529

- SYI_FRATN

UniProt

A0Q529 - SYI_FRATN

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Protein
Isoleucine--tRNA ligase
Gene
ileS, FTN_0441
Organism
Francisella tularensis subsp. novicida (strain U112)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Aminoacyl-adenylate By similarity
Binding sitei602 – 6021ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFNOV401614:GC4M-440-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:FTN_0441
OrganismiFrancisella tularensis subsp. novicida (strain U112)
Taxonomic identifieri401614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000000762: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022068Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi401614.FTN_0441.

Structurei

3D structure databases

ProteinModelPortaliA0Q529.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi599 – 6035"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0Q529-1 [UniParc]FASTAAdd to Basket

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MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK    50
FVLHDGPPYA NGSIHVGHAV NKILKDIIIK SKTLSGYDAP FTPTWDCHGL 100
PIELQVEKKH GKAGQSISED DFRKECRKYA KKQVEIQKKD FKRLGVLGDW 150
EQPYLTMNFD YEANMIRTLA KIIENGHLSK GFKPVHWCTD CGSALAEAEV 200
EYADKISPAI DVKFKIKDKD KLAQAFGLDS LNHDAFAIIW TTTPWTLPAN 250
QAIAVNNQLN YSLIKIEDFY IILAENLVEQ TLKRYAIENA QIIATTTGDK 300
LTGIIAEHPF YSRHVPILHG DHVTDDSGTG LVHTAPTHGV DDFTLGKEHN 350
LSMEIFVKGN GCYSENTKLF AGEFIFKAND RIIELLGEKK RLMNSDKIKH 400
SYPHCWRHKT PLMFRATPQW FISMEKQGLR NKALQAIKET SWAPSWGQAR 450
IEGMVKDRPD WCISRQRTWG VPLPLFIHKE TEELHPNTIE ILHKVAEKIE 500
KGGIEAWFNA DDCEFITETA QYKSVKDTLD VWFDSGSSSM CILDLDKRLS 550
YPADLYLEGS DQHRGWFQTS LLVAMSAKGS QPYKEVFTHG FVVDEHGRKM 600
SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR 650
RLRNTARFLL SNLDGFNPAT DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY 700
DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKTDGH PRKSAQTAIY 750
HIVHALVRWM APILSFTADE IWDATPKTTD LPIQLCEWYT GLKSFDQDAE 800
LDLEYWAKIQ EIRSEVNRVL EIKRNEDVVK ASLEAEITIY ADKDNYKLLE 850
KLGNELRFLL ISSKADLKVI EESTSSSIAA NIPGLSIEIT KIEEPKCERC 900
WHRSSTVGDN PQYKDICSRC VENITTEAGE SREFA 935
Length:935
Mass (Da):106,793
Last modified:January 9, 2007 - v1
Checksum:i566B71B95EBB28D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000439 Genomic DNA. Translation: ABK89344.1.
RefSeqiYP_898098.1. NC_008601.1.

Genome annotation databases

EnsemblBacteriaiABK89344; ABK89344; FTN_0441.
GeneIDi4548091.
KEGGiftn:FTN_0441.
PATRICi17933963. VBIFraNov128299_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000439 Genomic DNA. Translation: ABK89344.1 .
RefSeqi YP_898098.1. NC_008601.1.

3D structure databases

ProteinModelPortali A0Q529.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 401614.FTN_0441.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK89344 ; ABK89344 ; FTN_0441 .
GeneIDi 4548091.
KEGGi ftn:FTN_0441.
PATRICi 17933963. VBIFraNov128299_0461.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci FNOV401614:GC4M-440-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: U112.

Entry informationi

Entry nameiSYI_FRATN
AccessioniPrimary (citable) accession number: A0Q529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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