Reviewed, UniProtKB/Swiss-Prot A0Q3W9 (TPMT_FRATN)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	FTN_0023

Organism

Francisella tularensis subsp. novicida (strain U112) [Complete proteome] [HAMAP]

Taxonomic identifier

401614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047204
Sites
	Binding site	16	1	S-adenosyl-L-methionine By similarity
	Binding site	51	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	72	1	S-adenosyl-L-methionine By similarity
	Binding site	131	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0Q3W9-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 345A2DE8E4F103E3	FASTA	226	26,339
	10 20 30 40 50 60 MNKLETNNNQ YWLDRWQNDD VGFCQESPNE FLVKHFSKLN INDSSVCLIP MCGCSIDMLF 70 80 90 100 110 120 FLSKGVKVVG IELSEKAVLS FFSQNSINYE VIHGNDYKLY KGDDIEIYVA DIFNLPKIAN 130 140 150 160 170 180 NLPAFDIWYD RGAYIALPND LRTNYAKMML EVCPNNTQIL LLVMEHDKKS QTPPYSVTQA 190 200 210 220 ELIKNFSAKI KFELIDSKQR DNIPDYRKAE GMTKQYYTTY LRKKQY

References

[1]

"Comparison of Francisella tularensis genomes reveals evolutionary events associated with the emergence of human pathogenic strains." Rohmer L., Fong C., Abmayr S., Wasnick M., Larson Freeman T.J., Radey M., Guina T., Svensson K., Hayden H.S., Jacobs M., Gallagher L.A., Manoil C., Ernst R.K., Drees B., Buckley D., Haugen E., Bovee D., Zhou Y. , Chang J., Levy R., Lim R., Gillett W., Guenthener D., Kang A., Shaffer S.A., Taylor G., Chen J., Gallis B., D'Argenio D.A., Forsman M., Olson M.V., Goodlett D.R., Kaul R., Miller S.I., Brittnacher M.J. Genome Biol. 8:RESEARCH102.1-RESEARCH102.16(2007) [PubMed: 17550600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000439 Genomic DNA. Translation: ABK88934.1.
RefSeq	YP_897688.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0Q3W9.
Genome annotation databases
GeneID	4548515.
GenomeReviews	Gene locus FTN_0023 in contig CP000439_GR.
KEGG	ftn:FTN_0023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PPFAVSP.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_FRATN

Accession

Primary (citable) accession number: A0Q3W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)