A0Q3H1 (KHSE_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	NT01CX_0707

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Homoserine kinase HAMAP MF_00384		PRO_1000049125
Regions
	Nucleotide binding	85 – 95	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0Q3H1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A344844886806562	FASTA	299	33,450
	10 20 30 40 50 60 MIKVKVPGTT ANMGPGFDSF GMALDIYNEI TVEEIESGFE MLQEGELSEI PLAENLIYTT 70 80 90 100 110 120 FLNTLNKHNY KYKGFRINLS KCDVPMSRGL GSSATCIVGG IFAANSIMGN VMSFDEIIKE 130 140 150 160 170 180 AVSIEGHPDN VVPAIVGGMT VSIMDKDNVI YSNVTVPDRL RAFVMIPNYK LGTEEARGVL 190 200 210 220 230 240 PDSYTREECV FNISRAAMLV NVMNNGEIEK LRLCMQDKIH QKYRGALIRN IDDIFKKAYE 250 260 270 280 290 FGSLAEFISG SGSTLIAFID KDNNEFYDRM KNFLDTLEDE WTVHLLKPNF TGAEIIKNR

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK62607.1.
RefSeq	YP_879173.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0Q3H1.
ModBase	Search...
Protein-protein interaction databases
STRING	A0Q3H1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4541120.
GenomeReviews	Gene locus NT01CX_0707 in contig CP000382_GR.
KEGG	cno:NT01CX_0707.
PATRIC	19481968. VBICloNov112828_2211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
HOGENOM	HBG646290.
OMA	NNALMST.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	CNOV386415:NT01CX_0707-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CLONN

Accession

Primary (citable) accession number: A0Q3H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program