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A0Q3B0

- SYI_CLONN

UniProt

A0Q3B0 - SYI_CLONN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium novyi (strain NT)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCNOV386415:GH98-2210-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NT01CX_0646
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008220: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038Isoleucine--tRNA ligasePRO_1000070894Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi386415.NT01CX_0646.

Structurei

3D structure databases

ProteinModelPortaliA0Q3B0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi590 – 5945"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0Q3B0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNKIETSGN FVNMEEKIAK LWKDKDVIQK SFDRNEDGEY FTFYDGPPTA
60 70 80 90 100
NGKPHVGHVL TRVMKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL
110 120 130 140 150
GISGKPQIEE YGVEKFVKEC KDSVFKYTSL WKDMSEKLGF WVDMDNPYVT
160 170 180 190 200
YHNTYIESVW WALKTMWEKD LLYKGHRVTP YCPRCGTALS SHEVAQGYKD
210 220 230 240 250
VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK AYDYVEVEQN
260 270 280 290 300
GEHLILAKDL AEKVLEGEYK VVKEFKGEEL VGTEYEQLFK FEVPDKKAFY
310 320 330 340 350
VVHADYVTLT DGTGIVHTAP AYGEDDNMTG KKYDLPLINL VDGEGKFVDS
360 370 380 390 400
VTPWKGMFVK KADPKILEFM KENGSLYKSE KFTHSYPHCW RCDTPLLYYP
410 420 430 440 450
KDSWFVRMTS LRDKLLENNN KINWNPDNIR TGRFGKFLEN VIDWGISRDR
460 470 480 490 500
YWGTPLPIWE CECGHRECIG SIEELKEKGI NVPDDIELHK PYIDGVKLTC
510 520 530 540 550
PHCGKEMTRT NEVIDCWFDS GSMPFAQHHY PFENKEVFEK TFPAQFISEA
560 570 580 590 600
VDQTRGWFYS LLAISTALFD TNSYENCIVL GHVLDKHGLK MSKSKGNVVD
610 620 630 640 650
PFDVLQNEGA DATRWHFYTA SAPWLPTRFS EDDVKETQRK FLSTLWNVYS
660 670 680 690 700
FYVLYAELDQ FNPLEYKDFV SENVMDKWIL SKLNTLIKTV EEDLDNYKIT
710 720 730 740 750
EAALELEDFV DELSNWYVRR NRSRFWSTEL TDDKIGAYKT LYTVLTTIVK
760 770 780 790 800
VAAPFVPFMA EEIYQNLVVN LDKDAIESVH LCTWPEYDLS VVDNELEGQM
810 820 830 840 850
DLAYKIVKLG RSARNGANIK NRQPLSEMLI STKSLPDYYG DIIKEELNIK
860 870 880 890 900
KVEFGADLSK YVNFEIKPNL PVLGKAYGRY IPAIRKAISS MDQMELAQNI
910 920 930 940 950
NNGKSVFINV DGLDEQIELT ENNLLVAMQG LEGFAFAGSS GVGVILETTI
960 970 980 990 1000
TEELREEGFV REILSKVQNL RKESGFEVAD KIKLYFAGNE TLEVVIKKFE
1010 1020 1030
EHIKKETLAV LISYNEDREY VDSKINGEEL KIAVEKQD
Length:1,038
Mass (Da):119,727
Last modified:January 9, 2007 - v1
Checksum:iC03996885F7BF8F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60463.1.
RefSeqiYP_879112.1. NC_008593.1.

Genome annotation databases

EnsemblBacteriaiABK60463; ABK60463; NT01CX_0646.
GeneIDi4540257.
KEGGicno:NT01CX_0646.
PATRICi19481846. VBICloNov112828_2150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60463.1 .
RefSeqi YP_879112.1. NC_008593.1.

3D structure databases

ProteinModelPortali A0Q3B0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 386415.NT01CX_0646.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK60463 ; ABK60463 ; NT01CX_0646 .
GeneIDi 4540257.
KEGGi cno:NT01CX_0646.
PATRICi 19481846. VBICloNov112828_2150.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CNOV386415:GH98-2210-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NT.

Entry informationi

Entry nameiSYI_CLONN
AccessioniPrimary (citable) accession number: A0Q3B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3