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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium novyi (strain NT)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei593ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NT01CX_0646
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000008220 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000708941 – 1038Isoleucine--tRNA ligaseAdd BLAST1038

Proteomic databases

PRIDEiA0Q3B0

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi386415.NT01CX_0646

Structurei

3D structure databases

ProteinModelPortaliA0Q3B0
SMRiA0Q3B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi590 – 594"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiKMMAPFT
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0Q3B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNKIETSGN FVNMEEKIAK LWKDKDVIQK SFDRNEDGEY FTFYDGPPTA
60 70 80 90 100
NGKPHVGHVL TRVMKDLIPR YKVMKGYKVL RKAGWDTHGL PVELEIEKKL
110 120 130 140 150
GISGKPQIEE YGVEKFVKEC KDSVFKYTSL WKDMSEKLGF WVDMDNPYVT
160 170 180 190 200
YHNTYIESVW WALKTMWEKD LLYKGHRVTP YCPRCGTALS SHEVAQGYKD
210 220 230 240 250
VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK AYDYVEVEQN
260 270 280 290 300
GEHLILAKDL AEKVLEGEYK VVKEFKGEEL VGTEYEQLFK FEVPDKKAFY
310 320 330 340 350
VVHADYVTLT DGTGIVHTAP AYGEDDNMTG KKYDLPLINL VDGEGKFVDS
360 370 380 390 400
VTPWKGMFVK KADPKILEFM KENGSLYKSE KFTHSYPHCW RCDTPLLYYP
410 420 430 440 450
KDSWFVRMTS LRDKLLENNN KINWNPDNIR TGRFGKFLEN VIDWGISRDR
460 470 480 490 500
YWGTPLPIWE CECGHRECIG SIEELKEKGI NVPDDIELHK PYIDGVKLTC
510 520 530 540 550
PHCGKEMTRT NEVIDCWFDS GSMPFAQHHY PFENKEVFEK TFPAQFISEA
560 570 580 590 600
VDQTRGWFYS LLAISTALFD TNSYENCIVL GHVLDKHGLK MSKSKGNVVD
610 620 630 640 650
PFDVLQNEGA DATRWHFYTA SAPWLPTRFS EDDVKETQRK FLSTLWNVYS
660 670 680 690 700
FYVLYAELDQ FNPLEYKDFV SENVMDKWIL SKLNTLIKTV EEDLDNYKIT
710 720 730 740 750
EAALELEDFV DELSNWYVRR NRSRFWSTEL TDDKIGAYKT LYTVLTTIVK
760 770 780 790 800
VAAPFVPFMA EEIYQNLVVN LDKDAIESVH LCTWPEYDLS VVDNELEGQM
810 820 830 840 850
DLAYKIVKLG RSARNGANIK NRQPLSEMLI STKSLPDYYG DIIKEELNIK
860 870 880 890 900
KVEFGADLSK YVNFEIKPNL PVLGKAYGRY IPAIRKAISS MDQMELAQNI
910 920 930 940 950
NNGKSVFINV DGLDEQIELT ENNLLVAMQG LEGFAFAGSS GVGVILETTI
960 970 980 990 1000
TEELREEGFV REILSKVQNL RKESGFEVAD KIKLYFAGNE TLEVVIKKFE
1010 1020 1030
EHIKKETLAV LISYNEDREY VDSKINGEEL KIAVEKQD
Length:1,038
Mass (Da):119,727
Last modified:January 9, 2007 - v1
Checksum:iC03996885F7BF8F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA Translation: ABK60463.1
RefSeqiWP_011723091.1, NC_008593.1

Genome annotation databases

EnsemblBacteriaiABK60463; ABK60463; NT01CX_0646
GeneIDi4540257
KEGGicno:NT01CX_0646

Similar proteinsi

Entry informationi

Entry nameiSYI_CLONN
AccessioniPrimary (citable) accession number: A0Q3B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health