Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A0Q323 (DDL_CLONN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:NT01CX_0559
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP]
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030441

Regions

Domain129 – 335207ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2881Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 2 By similarity
Metal binding3041Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A0Q323 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: F0FBBCDF27ACDE8B

FASTA34338,355
        10         20         30         40         50         60 
MKKKVAILFG GQSTEHEVSR VSASSVLKNI DLSKYDVYPI GITKDGKWFE YTGAIDKIES 

        70         80         90        100        110        120 
GEWEKDEFYK NPNGQEILFN REVDVVFPVM HGLYGEDGTI QGLCKLLTIP CVGPGVMSSA 

       130        140        150        160        170        180 
VCMDKVYTKY VLENFGVKQA DYVVVNAHDY KNNKMDIIST IENKLGYDVF IKPSNSGSSV 

       190        200        210        220        230        240 
GISKAHNREE LEAGLEEALK FDRKVLVEVA LNAREIEVAV LGNDEPVAAT PGEIVPANEF 

       250        260        270        280        290        300 
YDYEAKYSNA QSKLLLPANL SPEKLEKVKE LAVRIFKMLD CAGMSRVDFL VDKETEEVYL 

       310        320        330        340 
NEINTIPGFT KISMYPKMWQ AEGKAYGELI SEIIELAVER DNK 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK62523.1.
RefSeqYP_879025.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0Q323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_0559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK62523; ABK62523; NT01CX_0559.
GeneID4541385.
KEGGcno:NT01CX_0559.
PATRIC19481672. VBICloNov112828_2063.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCNOV386415:GH98-2123-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CLONN
AccessionPrimary (citable) accession number: A0Q323
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways