A0Q2X7 (SECA_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein translocase subunit SecA

Gene names

Name:	secA
Ordered Locus Names:	NT01CX_0509

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium novyi

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane By similarity. HAMAP-Rule MF_01382
Cofactor	May bind 1 zinc ion per subunit Potential.
Subunit structure	Monomer and homodimer By similarity. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved By similarity. HAMAP-Rule MF_01382
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: Distribution is 50-50 By similarity. HAMAP-Rule MF_01382
Sequence similarities	Belongs to the SecA family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: HAMAP protein import Inferred from electronic annotation. Source: InterPro protein targeting Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Protein translocase subunit SecA HAMAP-Rule MF_01382		PRO_0000320781
Regions
	Nucleotide binding	100 – 107	8	ATP Potential
Sites
	Metal binding	820	1	Zinc Potential
	Metal binding	822	1	Zinc Potential
	Metal binding	831	1	Zinc Potential
	Metal binding	832	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A0Q2X7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 0F58ACF6A12C0CE9	FASTA	834	95,455
	10 20 30 40 50 60 MGLFEKIFGT YSSREIKKII PIINKIDSYE EEFKKLTDEE LRNKTDEFKD MLAKGKTLDD 70 80 90 100 110 120 ILPEAFAVAR EASARVLGMR HFREQLIGGI VLHQGRISEM KTGEGKTLVA TLPAYLNALS 130 140 150 160 170 180 GKGVHVITVN DYLAKRDRDQ MSQLYGFLGL TTGVIVHDLD NEQRREAYNC DITYGTNNEF 190 200 210 220 230 240 GFDYLRDNMV IYKEERVQRK LNFCIVDEVD SILIDEARTP LIISGEGDNS TDFYKVADFF 250 260 270 280 290 300 AKTLKEDDYT VDEKTNSVIL TEQGIEKAEK FFHIDNYGDG DNMQIQHHVV QALKANYTMK 310 320 330 340 350 360 RDKDYMVKDN EVIIVDEFTG RLMEGRRYSD GLHQAIEAKE NVKIQKESKT LATITFQNYF 370 380 390 400 410 420 RMYTKLSGMT GTAQTEEAEF REIYGLDVIV IPTHRPIARI DAPDVVYKSE KAKFKAIVNE 430 440 450 460 470 480 IAETYKKQQP VLVGTVSIEK SELLSDMLKR KGVPHQVLNA KYHEKEAEII SHAGEKGMVT 490 500 510 520 530 540 IATNMAGRGT DIKLGEGVEE VGGLKVIGTE RHESRRIDNQ LRGRSGRQGD PGYSRFYVSL 550 560 570 580 590 600 EDDLMRIFAS DRLQGVVEKL GLTDEDAIES RMVSNAIENA QKKVEGNNFD VRKSVLQYDD 610 620 630 640 650 660 VMNQQREVIY KQRSQVLEGE SLKEDIQEMI RSVISEAVDA HMSGLDETLE EDLEKLLAYL 670 680 690 700 710 720 QEIYLPKNVV TVDELKIKSD DEIKEILIDI AEKMYSEKEE EVTPERMREI ESVILLRIVD 730 740 750 760 770 780 TKWMDHIDNM DHLRQGMGLR AYRQQDPVQA YQFEGSEMFD EMINGIKTDT VKYLFHIQVE 790 800 810 820 830 KNIERERVAR ETSTNINDGE GGSHEPIKRK EEKIGRNDLC PCGSGKKYKN CCGR

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK61305.1.
RefSeq	YP_878979.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0Q2X7.
SMR	A0Q2X7. Positions 1-796.
ModBase	Search...
Protein-protein interaction databases
STRING	386415.NT01CX_0509.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK61305; ABK61305; NT01CX_0509.
GeneID	4541153.
KEGG	cno:NT01CX_0509.
PATRIC	19481574. VBICloNov112828_2018.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0653.
HOGENOM	HOG000218169.
KO	K03070.
OMA	ERFFILQ.
ProtClustDB	PRK12904.
Enzyme and pathway databases
BioCyc	CNOV386415:GH98-2073-MONOMER.
Family and domain databases
Gene3D	1.10.3060.10. 1 hit. 3.90.1440.10. 1 hit.
HAMAP	MF_01382. SecA.
InterPro	IPR027417. P-loop_NTPase. IPR004027. SEC_C_motif. IPR000185. SecA. IPR020937. SecA_CS. IPR011115. SecA_DEAD. IPR014018. SecA_motor_DEAD. IPR011130. SecA_preprotein_X-link_dom. IPR011116. SecA_Wing/Scaffold. [Graphical view]
Pfam	PF02810. SEC-C. 1 hit. PF07517. SecA_DEAD. 1 hit. PF01043. SecA_PP_bind. 1 hit. PF07516. SecA_SW. 1 hit. [Graphical view]
PRINTS	PR00906. SECA.
SMART	SM00957. SecA_DEAD. 1 hit. SM00958. SecA_PP_bind. 1 hit. [Graphical view]
SUPFAM	SSF81767. SecA_PP_bd. 1 hit. SSF81886. SecA_SW. 1 hit. SSF52540. SSF52540. 2 hits.
TIGRFAMs	TIGR00963. secA. 1 hit.
PROSITE	PS01312. SECA. 1 hit. PS51196. SECA_MOTOR_DEAD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECA_CLONN

Accession

Primary (citable) accession number: A0Q2X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 9, 2007
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program