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A0Q299 (THIM_CLONN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:NT01CX_0247
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP]
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000336552

Sites

Binding site441Substrate; via amide nitrogen By similarity
Binding site1201ATP By similarity
Binding site1661ATP By similarity
Binding site1931Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A0Q299 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 483A0CDBEBAF635B

FASTA26528,656
        10         20         30         40         50         60 
MLENICINFK KVKEKTPLVH SITNYVTIND CANMLLAYGA SPAMVESFDE SYDFAKLASC 

        70         80         90        100        110        120 
IYINLGTLTR EQEQSILMVC ISAKNNNIPV VLDPVACGAV PHKIALIEKL FEIGRIDIIK 

       130        140        150        160        170        180 
GNIGEIKSLA GYNAKTRGVD SIDNGNDSID ACISLAKKYK CIVAATGVKD IVTDGKRTAL 

       190        200        210        220        230        240 
IENGSKMLTL ITGAGCMVGA LTAATAGVED DKFIATITSI LSMNIAAEHT EKEVIGPGSF 

       250        260 
KVKLIDNIYL LKENHLRKEG KIKWI 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK60473.1.
RefSeqYP_878751.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0Q299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_0247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK60473; ABK60473; NT01CX_0247.
GeneID4539739.
KEGGcno:NT01CX_0247.
PATRIC19481044. VBICloNov112828_1787.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAVSGTGCM.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycCNOV386415:GH98-1814-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLONN
AccessionPrimary (citable) accession number: A0Q299
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways