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A0Q1J3 (A0Q1J3_CLONN) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485

Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:purF EMBL ABK62149.1
Ordered Locus Names:NT01CX_2422 EMBL ABK62149.1
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP] EMBL ABK62149.1
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site131For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2491Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3951Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4461Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4491Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
A0Q1J3 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 6B73AA044023606A

FASTA45850,209
        10         20         30         40         50         60 
MNKIEIDKFK DECGVFGIYS KSKLNVASVT YYGLYALQHR GEESAGIVVS DGEKLTCIKD 

        70         80         90        100        110        120 
MGLVSDVFNE EIINNMKGKM AIGHVRYSTF GESTISNAQP LLSSFKLGSI AIAHNGTLIN 

       130        140        150        160        170        180 
AEKIKQELEE KGVLFQTSID SEVILNLIAR SLKSDIEESI KDAMKEVKGS YGIVILTKDK 

       190        200        210        220        230        240 
LIGVRDPNGI RPLCIGKMED DYVICSESCA LNCIGAEFIR DVEPGEMVIV DENGLRSFKF 

       250        260        270        280        290        300 
EENIKHKTCA FEYIYFARPD SKMDGIEVYN SRVAAGEQLF KEAPADADIV IGVPDSGIPA 

       310        320        330        340        350        360 
AVGYAKASGI PYGIGLVKNK YVGRTFIAPS QDLRERAVSV KLSPLKVNVE GKRVVLIDDS 

       370        380        390        400        410        420 
IVRGTTSRRL VEVLKRAGAK EIHFRLSSPV VTDPCYFGIA TPYKKDLIGA NMSVDEICKE 

       430        440        450 
IGADSLGYLS IEGLLKVLKD GKDEFCLGCF NGQYPVER 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A.Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT EMBL ABK62149.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK62149.1.
RefSeqYP_878495.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0Q1J3.
SMRA0Q1J3. Positions 13-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_2422.

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK62149; ABK62149; NT01CX_2422.
GeneID4539783.
KEGGcno:NT01CX_2422.
PATRIC19480510. VBICloNov112828_1525.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMASGIPYEM.
OrthoDBEOG6KT2Q1.

Enzyme and pathway databases

BioCycCNOV386415:GH98-1554-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA0Q1J3_CLONN
AccessionPrimary (citable) accession number: A0Q1J3
Entry history
Integrated into UniProtKB/TrEMBL: January 9, 2007
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)