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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Clostridium novyi (strain NT)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Note: Binds 1 magnesium ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei13 – 131For GATase activityUniRule annotation
Metal bindingi249 – 2491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi395 – 3951Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi446 – 4461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi449 – 4491Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. iron-sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. nucleoside metabolic process Source: InterPro
  3. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotationImported, Transferase

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

Iron, Iron-sulfurUniRule annotation, MagnesiumUniRule annotation, Metal-binding

Enzyme and pathway databases

BioCyciCNOV386415:GH98-1554-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:purFImported
Ordered Locus Names:NT01CX_2422Imported
OrganismiClostridium novyi (strain NT)Imported
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008220 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi386415.NT01CX_2422.

Structurei

3D structure databases

ProteinModelPortaliA0Q1J3.
SMRiA0Q1J3. Positions 13-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0Q1J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIEIDKFK DECGVFGIYS KSKLNVASVT YYGLYALQHR GEESAGIVVS
60 70 80 90 100
DGEKLTCIKD MGLVSDVFNE EIINNMKGKM AIGHVRYSTF GESTISNAQP
110 120 130 140 150
LLSSFKLGSI AIAHNGTLIN AEKIKQELEE KGVLFQTSID SEVILNLIAR
160 170 180 190 200
SLKSDIEESI KDAMKEVKGS YGIVILTKDK LIGVRDPNGI RPLCIGKMED
210 220 230 240 250
DYVICSESCA LNCIGAEFIR DVEPGEMVIV DENGLRSFKF EENIKHKTCA
260 270 280 290 300
FEYIYFARPD SKMDGIEVYN SRVAAGEQLF KEAPADADIV IGVPDSGIPA
310 320 330 340 350
AVGYAKASGI PYGIGLVKNK YVGRTFIAPS QDLRERAVSV KLSPLKVNVE
360 370 380 390 400
GKRVVLIDDS IVRGTTSRRL VEVLKRAGAK EIHFRLSSPV VTDPCYFGIA
410 420 430 440 450
TPYKKDLIGA NMSVDEICKE IGADSLGYLS IEGLLKVLKD GKDEFCLGCF

NGQYPVER
Length:458
Mass (Da):50,209
Last modified:January 8, 2007 - v1
Checksum:i6B73AA044023606A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK62149.1.
RefSeqiWP_011722489.1. NC_008593.1.
YP_878495.1. NC_008593.1.

Genome annotation databases

EnsemblBacteriaiABK62149; ABK62149; NT01CX_2422.
GeneIDi4539783.
KEGGicno:NT01CX_2422.
PATRICi19480510. VBICloNov112828_1525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK62149.1.
RefSeqiWP_011722489.1. NC_008593.1.
YP_878495.1. NC_008593.1.

3D structure databases

ProteinModelPortaliA0Q1J3.
SMRiA0Q1J3. Positions 13-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386415.NT01CX_2422.

Protein family/group databases

MEROPSiC44.A01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK62149; ABK62149; NT01CX_2422.
GeneIDi4539783.
KEGGicno:NT01CX_2422.
PATRICi19480510. VBICloNov112828_1525.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIAGVNVH.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciCNOV386415:GH98-1554-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NTImported.

Entry informationi

Entry nameiA0Q1J3_CLONN
AccessioniPrimary (citable) accession number: A0Q1J3
Entry historyi
Integrated into UniProtKB/TrEMBL: January 8, 2007
Last sequence update: January 8, 2007
Last modified: March 31, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.