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Protein

Acetate kinase

Gene

ackA

Organism
Clostridium novyi (strain NT)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg(2+). Can also accept Mn2+.UniRule annotation

Pathway: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Binding sitei14 – 141ATPUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Active sitei147 – 1471Proton donor/acceptorUniRule annotation
Sitei179 – 1791Transition state stabilizerUniRule annotation
Sitei240 – 2401Transition state stabilizerUniRule annotation
Metal bindingi383 – 3831MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi207 – 2115ATPUniRule annotation
Nucleotide bindingi282 – 2843ATPUniRule annotation
Nucleotide bindingi330 – 3345ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNOV386415:GH98-1356-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:NT01CX_2224
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008220 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Acetate kinasePRO_1000002224Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi386415.NT01CX_2224.

Structurei

3D structure databases

ProteinModelPortaliA0Q0Z5.
SMRiA0Q0Z5. Positions 1-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0282.
HOGENOMiHOG000288398.
KOiK00925.
OMAiKIITCHI.
OrthoDBiEOG69975F.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0Q0Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVINCGS SSLKYQLIDM TSEQPIAQGL VERIGIEGSV LTHKVNGKKY
60 70 80 90 100
KIEEEMKDHK KAIELVLNAL VNEEYGVIKN MEEISAVGHR VVHGGEKYAE
110 120 130 140 150
SVLIDSEVME ALEDFVKLAP LHNPPNIIGI NACKELMSST PMVAVFDTAF
160 170 180 190 200
HQTLPDYAYM YSLPYDLYEK HGIRKYGFHG TSHKYVSAMA AKVLGKNIED
210 220 230 240 250
LKLITCHLGN GSSLAAVKNG KCVDTSMGFT PLAGLTMGTR CGDIDPAIVT
260 270 280 290 300
FLIKELNYSV DEVNKIMNKE SGVLGISGIS SDFRDILKAA SEGNERAELA
310 320 330 340 350
LNIFKNKVIQ YIGAYTAVMG GVDAIIFTAG VGENSEPIRK RIISDLGFLG
360 370 380 390 400
IKLDEEKNKV MGETETISTE DSKVKVLAIP TNEELMIARD TKEIVEKNNI

K
Length:401
Mass (Da):43,982
Last modified:January 9, 2007 - v1
Checksum:iBADD5534819CF006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60556.1.
RefSeqiWP_011722294.1. NC_008593.1.
YP_878297.1. NC_008593.1.

Genome annotation databases

EnsemblBacteriaiABK60556; ABK60556; NT01CX_2224.
GeneIDi4541780.
KEGGicno:NT01CX_2224.
PATRICi19480116. VBICloNov112828_1328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60556.1.
RefSeqiWP_011722294.1. NC_008593.1.
YP_878297.1. NC_008593.1.

3D structure databases

ProteinModelPortaliA0Q0Z5.
SMRiA0Q0Z5. Positions 1-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386415.NT01CX_2224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK60556; ABK60556; NT01CX_2224.
GeneIDi4541780.
KEGGicno:NT01CX_2224.
PATRICi19480116. VBICloNov112828_1328.

Phylogenomic databases

eggNOGiCOG0282.
HOGENOMiHOG000288398.
KOiK00925.
OMAiKIITCHI.
OrthoDBiEOG69975F.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BioCyciCNOV386415:GH98-1356-MONOMER.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NT.

Entry informationi

Entry nameiACKA_CLONN
AccessioniPrimary (citable) accession number: A0Q0Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.