A0Q0Z5 (ACKA_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetate kinase
EC=2.7.2.1

Alternative name(s):
Acetokinase

Gene names

Name:	ackA
Ordered Locus Names:	NT01CX_2224

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium novyi

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction By similarity. HAMAP-Rule MF_00020
Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_00020
Cofactor	Mg2+. Can also accept Mn2+ By similarity. HAMAP-Rule MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_00020
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00020
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00020.
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	acetyl-CoA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway organic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Acetate kinase HAMAP-Rule MF_00020		PRO_1000002224
Regions
	Nucleotide binding	207 – 211	5	ATP By similarity
	Nucleotide binding	282 – 284	3	ATP By similarity
	Nucleotide binding	330 – 334	5	ATP By similarity
Sites
	Active site	147	1	Proton donor/acceptor By similarity
	Metal binding	7	1	Magnesium By similarity
	Metal binding	383	1	Magnesium By similarity
	Binding site	14	1	ATP By similarity
	Binding site	90	1	Substrate By similarity
	Site	179	1	Transition state stabilizer By similarity
	Site	240	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0Q0Z5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: BADD5534819CF006	FASTA	401	43,982
	10 20 30 40 50 60 MKILVINCGS SSLKYQLIDM TSEQPIAQGL VERIGIEGSV LTHKVNGKKY KIEEEMKDHK 70 80 90 100 110 120 KAIELVLNAL VNEEYGVIKN MEEISAVGHR VVHGGEKYAE SVLIDSEVME ALEDFVKLAP 130 140 150 160 170 180 LHNPPNIIGI NACKELMSST PMVAVFDTAF HQTLPDYAYM YSLPYDLYEK HGIRKYGFHG 190 200 210 220 230 240 TSHKYVSAMA AKVLGKNIED LKLITCHLGN GSSLAAVKNG KCVDTSMGFT PLAGLTMGTR 250 260 270 280 290 300 CGDIDPAIVT FLIKELNYSV DEVNKIMNKE SGVLGISGIS SDFRDILKAA SEGNERAELA 310 320 330 340 350 360 LNIFKNKVIQ YIGAYTAVMG GVDAIIFTAG VGENSEPIRK RIISDLGFLG IKLDEEKNKV 370 380 390 400 MGETETISTE DSKVKVLAIP TNEELMIARD TKEIVEKNNI K

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK60556.1.
RefSeq	YP_878297.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0Q0Z5.
SMR	A0Q0Z5. Positions 1-396.
ModBase	Search...
Protein-protein interaction databases
STRING	386415.NT01CX_2224.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK60556; ABK60556; NT01CX_2224.
GeneID	4541780.
KEGG	cno:NT01CX_2224.
PATRIC	19480116. VBICloNov112828_1328.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0282.
HOGENOM	HOG000288398.
KO	K00925.
OMA	QHLMHVE.
Enzyme and pathway databases
BioCyc	CNOV386415:GH98-1356-MONOMER.
UniPathway	UPA00340; UER00458.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase.
InterPro	IPR004372. Ac/Proprionate_kinase. IPR000890. Aliphatic_acid_kin_short-chain. IPR023865. Aliphatic_acid_kinase_CS. [Graphical view]
PANTHER	PTHR21060. PTHR21060. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. ackA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA_CLONN

Accession

Primary (citable) accession number: A0Q0Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	May 29, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program