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A0PZT1 (PBPA_CLONN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Penicillin-binding protein 1A

Short name=PBP1a

Including the following 2 domains:

  1. Penicillin-insensitive transglycosylase
    EC=2.4.2.-
    Alternative name(s):
    Peptidoglycan TGase
  2. Penicillin-sensitive transpeptidase
    EC=3.4.-.-
    Alternative name(s):
    DD-transpeptidase
Gene names
Name:pbpA
Ordered Locus Names:NT01CX_1810
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP]
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.

In the C-terminal section; belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Penicillin-binding protein 1A
PRO_0000321875

Regions

Topological domain1 – 2828Cytoplasmic Potential
Transmembrane29 – 4921Helical; Signal-anchor for type II membrane protein; Potential
Topological domain50 – 863814Extracellular Potential
Region71 – 248178Transglycosylase By similarity
Region392 – 674283Transpeptidase By similarity
Compositional bias18 – 258Poly-Lys

Sites

Active site4311Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PZT1 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: A0250C35592BC539

FASTA86395,824
        10         20         30         40         50         60 
MTENRDNKTS QSEKTTQKKK KKKFKAFKII LITFITLIVI SLVTAIGITL AIIKTSPDIN 

        70         80         90        100        110        120 
INEIIAASDA SKIYDDKGEL VDSIITSKKK ILVKYDEVPE NLINAFVSIE DERFFKHSGI 

       130        140        150        160        170        180 
DVKRIAGAFL IDMKNIVSGK PALQGASTIT QQLIKNTVFE THGNSLNDKL RRKVQEWYLA 

       190        200        210        220        230        240 
PKLEKEVGKK SIMEAYLNTI YLGGRAIGVG AAADQYFNVS IDKLDLVQCA FIAGLPQSPS 

       250        260        270        280        290        300 
VYYPYSRTSK KDPSKYINRT KTVLAKMKEN GYISQNEYIS ALAELDTSKS TVTNDESIQT 

       310        320        330        340        350        360 
LGQYTIHKPT NIDEKYNFEW FTRPAIERVK KDLKDIYNYS DDEIEKLLVN GNLKIYTTMN 

       370        380        390        400        410        420 
KDLQVSTQEI IDNDEKLNSL SSSKNNLVEP QASAVLTDYH TGEVKVIIGG RGTQPALAYN 

       430        440        450        460        470        480 
RATNAKVAAG SSIKPLTVYS AAIDSKLATA ATVLEDSPLP EAMSKKYSAP GTNWQPKNAN 

       490        500        510        520        530        540 
GVYSGYLGLR DALKNSVNVY AVKLEDKIGL NTGVKYGEKF GLTFDNVDKN SMAAIALGEL 

       550        560        570        580        590        600 
NRGTNTFTMA NAYGVFGNNG MYSNPRLYTK VLDRNGNVLL ETKTQATQVI SPEAAYIMYD 

       610        620        630        640        650        660 
LLKGPVKEGT ATRIQHTYHS DIPIAGKTGS STKFKNLWFC GLTPYYSGAV WIENKYGQSI 

       670        680        690        700        710        720 
YSSDAAALFG KIMNRAVENL PEKEIEMPEG IIKAEVDRVS GLLPTDLSYK DPRGSQVYTE 

       730        740        750        760        770        780 
LFIKGTVPTE QDNIHVSTKV NKYNGRVSGS YTPSFLTESK VFIKRQSDSE VPLDDDMYVL 

       790        800        810        820        830        840 
PDKDKKSSNK SKHNHNNDAK HDNTNNSEDA TNEASTEPSP NTDTVPEDST NNLDPTKNTE 

       850        860 
KKPSDKKNKK HVIKPIIRPK KHF 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK60403.1.
RefSeqYP_877883.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0PZT1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_1810.

Protein family/group databases

CAZyGT51. Glycosyltransferase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK60403; ABK60403; NT01CX_1810.
GeneID4540909.
KEGGcno:NT01CX_1810.
PATRIC19479286. VBICloNov112828_0913.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0744.
HOGENOMHOG000041137.
KOK05366.
OMAAFIADTM.
OrthoDBEOG6KDKQS.

Enzyme and pathway databases

BioCycCNOV386415:GH98-942-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNetSearch...

Entry information

Entry namePBPA_CLONN
AccessionPrimary (citable) accession number: A0PZT1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways