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Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium novyi (strain NT)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.By similarity
Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110Proton donor; for transglycosylase activityBy similarity1
Active sitei431Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase
Biological processAntibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciCNOV386415:G1G6M-931-MONOMER
UniPathwayiUPA00219

Protein family/group databases

CAZyiGT51 Glycosyltransferase Family 51

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129By similarity)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.16.4By similarity)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:NT01CX_1810
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000008220 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 49Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini50 – 863ExtracellularSequence analysisAdd BLAST814

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003218751 – 863Penicillin-binding protein 1AAdd BLAST863

Proteomic databases

PRIDEiA0PZT1

Interactioni

Protein-protein interaction databases

STRINGi386415.NT01CX_1810

Structurei

3D structure databases

ProteinModelPortaliA0PZT1
SMRiA0PZT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 248TransglycosylaseBy similarityAdd BLAST178
Regioni392 – 674TranspeptidaseBy similarityAdd BLAST283

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi18 – 25Poly-Lys8

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4 Bacteria
COG0744 LUCA
HOGENOMiHOG000041137
KOiK05366
OMAiWVGFNDS
OrthoDBiPOG091H01NC

Family and domain databases

Gene3Di1.10.3810.10, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR001264 Glyco_trans_51
IPR023346 Lysozyme-like_dom_sf
IPR036950 PBP_transglycosylase
IPR001460 PCN-bd_Tpept
PfamiView protein in Pfam
PF00912 Transgly, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF56601 SSF56601, 2 hits

Sequencei

Sequence statusi: Complete.

A0PZT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENRDNKTS QSEKTTQKKK KKKFKAFKII LITFITLIVI SLVTAIGITL
60 70 80 90 100
AIIKTSPDIN INEIIAASDA SKIYDDKGEL VDSIITSKKK ILVKYDEVPE
110 120 130 140 150
NLINAFVSIE DERFFKHSGI DVKRIAGAFL IDMKNIVSGK PALQGASTIT
160 170 180 190 200
QQLIKNTVFE THGNSLNDKL RRKVQEWYLA PKLEKEVGKK SIMEAYLNTI
210 220 230 240 250
YLGGRAIGVG AAADQYFNVS IDKLDLVQCA FIAGLPQSPS VYYPYSRTSK
260 270 280 290 300
KDPSKYINRT KTVLAKMKEN GYISQNEYIS ALAELDTSKS TVTNDESIQT
310 320 330 340 350
LGQYTIHKPT NIDEKYNFEW FTRPAIERVK KDLKDIYNYS DDEIEKLLVN
360 370 380 390 400
GNLKIYTTMN KDLQVSTQEI IDNDEKLNSL SSSKNNLVEP QASAVLTDYH
410 420 430 440 450
TGEVKVIIGG RGTQPALAYN RATNAKVAAG SSIKPLTVYS AAIDSKLATA
460 470 480 490 500
ATVLEDSPLP EAMSKKYSAP GTNWQPKNAN GVYSGYLGLR DALKNSVNVY
510 520 530 540 550
AVKLEDKIGL NTGVKYGEKF GLTFDNVDKN SMAAIALGEL NRGTNTFTMA
560 570 580 590 600
NAYGVFGNNG MYSNPRLYTK VLDRNGNVLL ETKTQATQVI SPEAAYIMYD
610 620 630 640 650
LLKGPVKEGT ATRIQHTYHS DIPIAGKTGS STKFKNLWFC GLTPYYSGAV
660 670 680 690 700
WIENKYGQSI YSSDAAALFG KIMNRAVENL PEKEIEMPEG IIKAEVDRVS
710 720 730 740 750
GLLPTDLSYK DPRGSQVYTE LFIKGTVPTE QDNIHVSTKV NKYNGRVSGS
760 770 780 790 800
YTPSFLTESK VFIKRQSDSE VPLDDDMYVL PDKDKKSSNK SKHNHNNDAK
810 820 830 840 850
HDNTNNSEDA TNEASTEPSP NTDTVPEDST NNLDPTKNTE KKPSDKKNKK
860
HVIKPIIRPK KHF
Length:863
Mass (Da):95,824
Last modified:January 9, 2007 - v1
Checksum:iA0250C35592BC539
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA Translation: ABK60403.1
RefSeqiWP_011721888.1, NC_008593.1

Genome annotation databases

EnsemblBacteriaiABK60403; ABK60403; NT01CX_1810
GeneIDi4540909
KEGGicno:NT01CX_1810
PATRICifig|386415.7.peg.913

Similar proteinsi

Entry informationi

Entry nameiPBPA_CLONN
AccessioniPrimary (citable) accession number: A0PZT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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