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Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium novyi (strain NT)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. penicillin binding Source: InterPro
  3. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of cell shape Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciCNOV386415:GH98-942-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1a
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:NT01CX_1810
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008220: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei29 – 4921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini50 – 863814ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Penicillin-binding protein 1APRO_0000321875Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi386415.NT01CX_1810.

Structurei

3D structure databases

ProteinModelPortaliA0PZT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 248178TransglycosylaseBy similarityAdd
BLAST
Regioni392 – 674283TranspeptidaseBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi18 – 258Poly-Lys

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
KOiK05366.
OMAiNDINETN.
OrthoDBiEOG6KDKQS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.

Sequencei

Sequence statusi: Complete.

A0PZT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENRDNKTS QSEKTTQKKK KKKFKAFKII LITFITLIVI SLVTAIGITL
60 70 80 90 100
AIIKTSPDIN INEIIAASDA SKIYDDKGEL VDSIITSKKK ILVKYDEVPE
110 120 130 140 150
NLINAFVSIE DERFFKHSGI DVKRIAGAFL IDMKNIVSGK PALQGASTIT
160 170 180 190 200
QQLIKNTVFE THGNSLNDKL RRKVQEWYLA PKLEKEVGKK SIMEAYLNTI
210 220 230 240 250
YLGGRAIGVG AAADQYFNVS IDKLDLVQCA FIAGLPQSPS VYYPYSRTSK
260 270 280 290 300
KDPSKYINRT KTVLAKMKEN GYISQNEYIS ALAELDTSKS TVTNDESIQT
310 320 330 340 350
LGQYTIHKPT NIDEKYNFEW FTRPAIERVK KDLKDIYNYS DDEIEKLLVN
360 370 380 390 400
GNLKIYTTMN KDLQVSTQEI IDNDEKLNSL SSSKNNLVEP QASAVLTDYH
410 420 430 440 450
TGEVKVIIGG RGTQPALAYN RATNAKVAAG SSIKPLTVYS AAIDSKLATA
460 470 480 490 500
ATVLEDSPLP EAMSKKYSAP GTNWQPKNAN GVYSGYLGLR DALKNSVNVY
510 520 530 540 550
AVKLEDKIGL NTGVKYGEKF GLTFDNVDKN SMAAIALGEL NRGTNTFTMA
560 570 580 590 600
NAYGVFGNNG MYSNPRLYTK VLDRNGNVLL ETKTQATQVI SPEAAYIMYD
610 620 630 640 650
LLKGPVKEGT ATRIQHTYHS DIPIAGKTGS STKFKNLWFC GLTPYYSGAV
660 670 680 690 700
WIENKYGQSI YSSDAAALFG KIMNRAVENL PEKEIEMPEG IIKAEVDRVS
710 720 730 740 750
GLLPTDLSYK DPRGSQVYTE LFIKGTVPTE QDNIHVSTKV NKYNGRVSGS
760 770 780 790 800
YTPSFLTESK VFIKRQSDSE VPLDDDMYVL PDKDKKSSNK SKHNHNNDAK
810 820 830 840 850
HDNTNNSEDA TNEASTEPSP NTDTVPEDST NNLDPTKNTE KKPSDKKNKK
860
HVIKPIIRPK KHF
Length:863
Mass (Da):95,824
Last modified:January 9, 2007 - v1
Checksum:iA0250C35592BC539
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60403.1.
RefSeqiWP_011721888.1. NC_008593.1.
YP_877883.1. NC_008593.1.

Genome annotation databases

EnsemblBacteriaiABK60403; ABK60403; NT01CX_1810.
GeneIDi4540909.
KEGGicno:NT01CX_1810.
PATRICi19479286. VBICloNov112828_0913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK60403.1.
RefSeqiWP_011721888.1. NC_008593.1.
YP_877883.1. NC_008593.1.

3D structure databases

ProteinModelPortaliA0PZT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386415.NT01CX_1810.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK60403; ABK60403; NT01CX_1810.
GeneIDi4540909.
KEGGicno:NT01CX_1810.
PATRICi19479286. VBICloNov112828_0913.

Phylogenomic databases

eggNOGiCOG0744.
HOGENOMiHOG000041137.
KOiK05366.
OMAiNDINETN.
OrthoDBiEOG6KDKQS.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciCNOV386415:GH98-942-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NT.

Entry informationi

Entry nameiPBPA_CLONN
AccessioniPrimary (citable) accession number: A0PZT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: February 4, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.