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A0PYK3 (SYL_CLONN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:NT01CX_1372
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP]
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length812 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 812812Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009328

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif572 – 5765"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5751ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PYK3 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: D95725B24357888A

FASTA81292,378
        10         20         30         40         50         60 
MGNYGTTVDE KWQKKWEESG LHNFDENAPG EKLYVLEMFS YPSAAKLHAG HWFNYGPTDS 

        70         80         90        100        110        120 
WARFKKMNGY NVFQPMGFDA FGLPAENFAI KTGIHPQDST MKNIENMEEQ LRSMGAMFNW 

       130        140        150        160        170        180 
DHEIITCLPD YYKWTQWVFL KLYEKGLAYR KNAPVNWCPS CNTVLANEQV LDGHCERCDS 

       190        200        210        220        230        240 
LVEKKALTQW FLKITDYADE LLEKLDGLDW PEKTKAMQKH WIGKSKGVEA TFKVENSDIT 

       250        260        270        280        290        300 
FDVFTTRVDT LNGVTYVVLA PENELVDSLT TEENKAAVEA YKIEAQKQSD IERQSSTREK 

       310        320        330        340        350        360 
TGVFTGSYAI NPINGNKVPI WVGDYVLATY GTGCVMAVPA HDERDYAFAT KYDLPIVRVI 

       370        380        390        400        410        420 
EGGDSLPFTE YGSLVNSGEF DGLYGEEAKE AIVKKLQEQK LGNWKVNYRL RDWLVSRQRY 

       430        440        450        460        470        480 
WGAPIPVVYC DKCGTVAVPE EQLPVELPYN IEFTPDGKSP LSKSEEFLHT TCPKCGGHAV 

       490        500        510        520        530        540 
RETDTLDTFV CSSWYYLRYV DNNNSEKAFD IDKVNKMLPV DKYVGGPEHA CMHLLYARFI 

       550        560        570        580        590        600 
TKALRDMGYL NFDEPFTSLT HQGLILGPDG LKMSKSKGNT IAPDDYIKEY GADVFRMYLM 

       610        620        630        640        650        660 
FGFAYSEGGA WSDDAIKSMS KFVDKVERIL ADSRTQMADS KNTKTTIEKA EKELNYTRHY 

       670        680        690        700        710        720 
AIQHVTEDTE KFQFNTAIAR IMEYTNSLSK YLNEENLNVE FLKEALTDYI KLLAPFAPHF 

       730        740        750        760        770        780 
SEEQWELLGN TSSVFTTSWP KFDPKALVKD EVEIAIQILG KIKARMNIAT NLTDEEIKEA 

       790        800        810 
ALNNETIKGL LEGKNIIKVI VVKGSLVNIV AK 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK61363.1.
RefSeqYP_877455.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0PYK3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_1372.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK61363; ABK61363; NT01CX_1372.
GeneID4541679.
KEGGcno:NT01CX_1372.
PATRIC19478402. VBICloNov112828_0480.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCNOV386415:GH98-504-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CLONN
AccessionPrimary (citable) accession number: A0PYK3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries