A0PYE7 (AROD_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	NT01CX_1316

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000043161
Sites
	Active site	143	1	Proton acceptor By similarity
	Active site	170	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PYE7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 9108D5328607117D	FASTA	253	28,470
	10 20 30 40 50 60 MKTVNIRGVI LGEGIPKVCT PLVGRSLKEL REEINLLKDI DCDLVEFRAD FFEHVENIQK 70 80 90 100 110 120 VKEVLLEIRE ALKEKPILFT FRSAKEGGER EVESEFYCKL NKEIIKTKLI DAIDIELFNE 130 140 150 160 170 180 EESILELIKI AHDEDVKVVM SNHDFHKTPP KEEMISRLVK MQELGADVTK IAVMPKGSSD 190 200 210 220 230 240 VLTLLEATND MKIKYAKTPF ITMSMKGVGM ISRISGEVFG SAVTFGASKK ASAPGQLQVK 250 ELKEILNVVH NVL

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK61917.1.
RefSeq	YP_877399.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0PYE7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PYE7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4541218.
GenomeReviews	Gene locus NT01CX_1316 in contig CP000382_GR.
KEGG	cno:NT01CX_1316.
PATRIC	19478290. VBICloNov112828_0424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
HOGENOM	HBG732926.
OMA	IAKIAVM.
Enzyme and pathway databases
BioCyc	CNOV386415:NT01CX_1316-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_CLONN

Accession

Primary (citable) accession number: A0PYE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program