A0PYB9 (A0PYB9_CLONN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555 EMBL ABK61012.1
Ordered Locus Names:	NT01CX_1288

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL ABK61012.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A0PYB9 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: E4AE442FB7B1D244	FASTA	339	39,284
	10 20 30 40 50 60 MTTQKKFIVK EGYKSSMDLK KTEIAIKQLK DFFERELAYK LNLIRVSAPL FVKESSGLND 70 80 90 100 110 120 NLNGIERPVA FDALDIKKEE IQIVHSLAKW KRMALHRYGF KPGEGLYTDM NAIRRDEELD 130 140 150 160 170 180 NIHSMYVDQW DWEKVIKKED RTEAKLKEIV EGIYEVFKST ENFVSDKYPE IDKTLPEKIT 190 200 210 220 230 240 FITSQELENM YPNLTPKERE NTITKEKGAV FLMKIGDTLA SGEKHDGRAP DYDDWSLNGD 250 260 270 280 290 300 ILFWYPVLNC ALELSSMGIR VDEDALKYQL KKANCEERAE LEFHKMLLEK KLPYTVGGGI 310 320 330 GQSRICMFFL RKAHIGEVQA SIWSEDVIKE CEELGITLL

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK61012.1.
RefSeq	YP_877371.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0PYB9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PYB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4541821.
GenomeReviews	Gene locus NT01CX_1288 in contig CP000382_GR.
KEGG	cno:NT01CX_1288.
PATRIC	19478234. VBICloNov112828_0396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0PYB9_CLONN

Accession

Primary (citable) accession number: A0PYB9

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)