A0PY95 (ALR_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	NT01CX_1264

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Alanine racemase HAMAP MF_01201		PRO_1000065979
Sites
	Active site	38	1	Proton acceptor; specific for D-alanine By similarity
	Active site	267	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	38	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PY95 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 523B69FF5F59C7D3	FASTA	387	43,171
	10 20 30 40 50 60 MFKHLRPVWA EINLDNLASN MKHIKELSNT KEIIGIVKAD AYGHGALDIV PTLIENGATA 70 80 90 100 110 120 LAVAVVSEGV ELRRGGIECP IMVLGFTPPS LIDMLLKHDI EQTVFSLDYA KELSKAAEKM 130 140 150 160 170 180 HKVAKIHIAV DTGMGRIGFL PNEQSIQDVK AISMLPNIKI KGMFSHFSTA DEKNKEYSAY 190 200 210 220 230 240 QLNQFNKFYE GLKRENVNIE TRHISNSAAI MDLPETIFEG VRPGIILYGY YPSNEVDKTK 250 260 270 280 290 300 LELKPVMQLK TNVVHIKKIP SGEYISYGRK FKTDRESLIA TLPVGYADGY TRLLFGKAKV 310 320 330 340 350 360 IINGQLAPVV GRICMDQCMV DITDIKGDIK VGEEVILIGE KNGVKIDADD IAEMLGTINY 370 380 EVICMISKRV PRVYIKNGEV IKVRNYI

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK61810.1.
RefSeq	YP_877347.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0PY95.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PY95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4540795.
GenomeReviews	Gene locus NT01CX_1264 in contig CP000382_GR.
KEGG	cno:NT01CX_1264.
PATRIC	19478190. VBICloNov112828_0374.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	TRKEDAN.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	CNOV386415:NT01CX_1264-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_CLONN

Accession

Primary (citable) accession number: A0PY95

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program