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A0PXN0 (FTHS_CLONN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:NT01CX_1042
OrganismClostridium novyi (strain NT) [Complete proteome] [HAMAP]
Taxonomic identifier386415 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000300519

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PXN0 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 8BCA88252C288010

FASTA55660,126
        10         20         30         40         50         60 
MKSDIEIAQS ANMKKIADIA KELGLGEDDI ELYGKYKCKI SLDVLKNKHN EADGKLILVT 

        70         80         90        100        110        120 
AINPTPAGEG KSTITVGLGQ ALCKLHKKAV IALREPSLGP VFGIKGGAAG GGYSQVVPME 

       130        140        150        160        170        180 
DINLHFTGDM HAITSANNLL AAAIDNHIHQ GNTLKIDSRR ILFKRVMDMN DRALRHIVVG 

       190        200        210        220        230        240 
MGGKVNGFLR EDGFTITVAS EIMAILCLSN SLMDLKERFG NILVAYNLDG EPIYCKDLNV 

       250        260        270        280        290        300 
HGAMAMLMKD AIKPNLVQTL ENTPAIIHGG PFANIAHGCN SILATKMAMK LGDYAITEAG 

       310        320        330        340        350        360 
FGADLGAEKF LDIKCRYGNL KPDCIVVVAT IRALKHHGGV SKDELSTPDI EALSKGVSNL 

       370        380        390        400        410        420 
QKQIENMKKY GVPVVVAINK FITDSDEEVE FIKEFCDKQG VEVALAEVWE KGGEGGIALA 

       430        440        450        460        470        480 
EKVINVLDTQ KSNFKCLYDE KLSIKEKMDI IAREIYGASG VDYDKSAEKD IKDIEKLGLD 

       490        500        510        520        530        540 
KLPICVAKTQ YSLSDNPSLL GKPEDFRVNV REVKVSNGAG FIVVLTGNIM TMPGLPKVPA 

       550 
ANKMDILEGG TIQGLF 

« Hide

References

[1]"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT."
Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S.
Nat. Biotechnol. 24:1573-1580(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000382 Genomic DNA. Translation: ABK61005.1.
RefSeqYP_877132.1. NC_008593.1.

3D structure databases

ProteinModelPortalA0PXN0.
SMRA0PXN0. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING386415.NT01CX_1042.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK61005; ABK61005; NT01CX_1042.
GeneID4541815.
KEGGcno:NT01CX_1042.
PATRIC19477744. VBICloNov112828_0158.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycCNOV386415:GH98-175-MONOMER.
UniPathwayUPA00193.

Family and domain databases

HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLONN
AccessionPrimary (citable) accession number: A0PXN0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 9, 2007
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways