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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Clostridium novyi (strain NT)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNOV386415:GH98-175-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:NT01CX_1042
OrganismiClostridium novyi (strain NT)
Taxonomic identifieri386415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008220 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000300519Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi386415.NT01CX_1042.

Structurei

3D structure databases

ProteinModelPortaliA0PXN0.
SMRiA0PXN0. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0PXN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDIEIAQS ANMKKIADIA KELGLGEDDI ELYGKYKCKI SLDVLKNKHN
60 70 80 90 100
EADGKLILVT AINPTPAGEG KSTITVGLGQ ALCKLHKKAV IALREPSLGP
110 120 130 140 150
VFGIKGGAAG GGYSQVVPME DINLHFTGDM HAITSANNLL AAAIDNHIHQ
160 170 180 190 200
GNTLKIDSRR ILFKRVMDMN DRALRHIVVG MGGKVNGFLR EDGFTITVAS
210 220 230 240 250
EIMAILCLSN SLMDLKERFG NILVAYNLDG EPIYCKDLNV HGAMAMLMKD
260 270 280 290 300
AIKPNLVQTL ENTPAIIHGG PFANIAHGCN SILATKMAMK LGDYAITEAG
310 320 330 340 350
FGADLGAEKF LDIKCRYGNL KPDCIVVVAT IRALKHHGGV SKDELSTPDI
360 370 380 390 400
EALSKGVSNL QKQIENMKKY GVPVVVAINK FITDSDEEVE FIKEFCDKQG
410 420 430 440 450
VEVALAEVWE KGGEGGIALA EKVINVLDTQ KSNFKCLYDE KLSIKEKMDI
460 470 480 490 500
IAREIYGASG VDYDKSAEKD IKDIEKLGLD KLPICVAKTQ YSLSDNPSLL
510 520 530 540 550
GKPEDFRVNV REVKVSNGAG FIVVLTGNIM TMPGLPKVPA ANKMDILEGG

TIQGLF
Length:556
Mass (Da):60,126
Last modified:January 9, 2007 - v1
Checksum:i8BCA88252C288010
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK61005.1.
RefSeqiWP_011721153.1. NC_008593.1.

Genome annotation databases

EnsemblBacteriaiABK61005; ABK61005; NT01CX_1042.
GeneIDi4541815.
KEGGicno:NT01CX_1042.
PATRICi19477744. VBICloNov112828_0158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000382 Genomic DNA. Translation: ABK61005.1.
RefSeqiWP_011721153.1. NC_008593.1.

3D structure databases

ProteinModelPortaliA0PXN0.
SMRiA0PXN0. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi386415.NT01CX_1042.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK61005; ABK61005; NT01CX_1042.
GeneIDi4541815.
KEGGicno:NT01CX_1042.
PATRICi19477744. VBICloNov112828_0158.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciCNOV386415:GH98-175-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NT.

Entry informationi

Entry nameiFTHS_CLONN
AccessioniPrimary (citable) accession number: A0PXN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.