Reviewed, UniProtKB/Swiss-Prot A0PXB6 (FABH_CLONN)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase 3
    EC=2.3.1.41
Alternative name(s):
    3-oxoacyl-[acyl-carrier-protein] synthase III
    Beta-ketoacyl-ACP synthase III
      Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	NT01CX_0921

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/acpP and fabH By similarity.
Sequence similarities	Belongs to the fabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000056347
Regions
	Region	251 – 255	5	ACP-binding By similarity
Sites
	Active site	112	1	By similarity
	Active site	250	1	By similarity
	Active site	280	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PXB6-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 98FFA180BDC1D1B8	FASTA	324	35,761
	10 20 30 40 50 60 MYNVKIENTG RYVPDNIVTN EDISKIVDTN DKWIRERTGI KERRISKGEN TSHMAIKSAK 70 80 90 100 110 120 DVLRKSSIKA DELDLIIVAT CTPDCFVPST ACIVQDAIGA TKATCFDINA ACTGFMYALS 130 140 150 160 170 180 VASQFIKSGQ SKNALVIGAE TLSKMINWQD RGTCVLFADG AGAAILTRSE EVGLISQYTC 190 200 210 220 230 240 SDGTGGKFLK CDALPVENPY VSEKTEFLNK LSMEGREVFK FAVNAMIDSV HKVLEKTDYT 250 260 270 280 290 300 IEDIDYIVPH QANIRIIEYV AKKLKVSQDK FYINLHRYGN TSGASIPIAL DEMNEKNMLK 310 320 KGDKIILVGF GGGLTFGAHL IQWN

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000382 Genomic DNA. Translation: ABK60947.1.
RefSeq	YP_877018.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0PXB6.
Genome annotation databases
GeneID	4541887.
GenomeReviews	Gene locus NT01CX_0921 in contig CP000382_GR.
KEGG	cno:NT01CX_0921.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YIVPHQA.
Family and domain databases
HAMAP	MF_01815. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00747. fabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_CLONN

Accession

Primary (citable) accession number: A0PXB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)