A0PXA4 (PROA_CLONN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	NT01CX_0901

Organism

Clostridium novyi (strain NT) [Complete proteome] [HAMAP]

Taxonomic identifier

386415 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049944

Sequences

	Sequence		Length	Mass (Da)
	A0PXA4 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 2E0C10009845E73D	FASTA	417	45,237
	10 20 30 40 50 60 MNIENYVIET ASLAKSAARK MSIVSTVTKN NALNAMADAL IENTNAIIEA NKKDMENGRE 70 80 90 100 110 120 KGLTESLLDR LLLDEARIKS MAQGLRDVAS LEDPIGEVIR MWRRPNNLKI GQIRVPLGVI 130 140 150 160 170 180 GIIYEARPNV TVDAAALCVK SGNAVILRGG SEAINSNTTV ARIISEAATK AGLPEGAINL 190 200 210 220 230 240 IENPSRDAVN VMMKLNDYID VLIPRGGAGL INAVVKNATV PVIQTGVGNC HVFVDASADL 250 260 270 280 290 300 EMAANIVINA KTQRPAVCNA MESLLVHKDI ADKFLPYLAE KLKPLNVEIK GCIRTQSLVE 310 320 330 340 350 360 GATEATEEDW AKEYLDFKFA SKVVDSLDEA LDHIYKYSTK HSEVIVTNNY ENSQRFLAEV 370 380 390 400 410 DAAAVYVNAS SRFTDGSEFG FGAEIGISTQ KLHARGPMGL KELTSSKYII YGEGQIR

References

[1]

"The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT." Bettegowda C., Huang X., Lin J., Cheong I., Kohli M., Szabo S.A., Zhang X., Diaz L.A. Jr., Velculescu V.E., Parmigiani G., Kinzler K.W., Vogelstein B., Zhou S. Nat. Biotechnol. 24:1573-1580(2006) [PubMed: 17115055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000382 Genomic DNA. Translation: ABK60892.1.
RefSeq	YP_877006.1. NC_008593.1.
3D structure databases
ProteinModelPortal	A0PXA4.
SMR	A0PXA4. Positions 7-417.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PXA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4541291.
GenomeReviews	Gene locus NT01CX_0901 in contig CP000382_GR.
KEGG	cno:NT01CX_0901.
PATRIC	19477464. VBICloNov112828_0033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	CNOV386415:NT01CX_0901-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CLONN

Accession

Primary (citable) accession number: A0PXA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program