ID   MPRA_MYCUA              Reviewed;         232 AA.
AC   A0PWB4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   27-MAR-2024, entry version 96.
DE   RecName: Full=Response regulator MprA;
DE   AltName: Full=Mycobacterial persistence regulator A;
GN   Name=mprA; OrderedLocusNames=MUL_4701;
OS   Mycobacterium ulcerans (strain Agy99).
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium ulcerans group.
OX   NCBI_TaxID=362242;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Agy99;
RX   PubMed=17210928; DOI=10.1101/gr.5942807;
RA   Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T.,
RA   Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J.,
RA   Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.,
RA   Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.;
RT   "Reductive evolution and niche adaptation inferred from the genome of
RT   Mycobacterium ulcerans, the causative agent of Buruli ulcer.";
RL   Genome Res. 17:192-200(2007).
CC   -!- FUNCTION: Member of the two-component regulatory system MprB/MprA which
CC       contributes to maintaining a balance among several systems involved in
CC       stress resistance and is required for establishment and maintenance of
CC       persistent infection in the host. Functions as a transcriptional
CC       regulator that recognizes a 19-bp nucleotide motif comprizing two
CC       loosely conserved 8-bp direct DNA-binding motif repeats separated by a
CC       3-bp spacer region (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated and dephosphorylated by MprB. {ECO:0000250}.
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DR   EMBL; CP000325; ABL06633.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0PWB4; -.
DR   SMR; A0PWB4; -.
DR   KEGG; mul:MUL_4701; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_1_11; -.
DR   Proteomes; UP000000765; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR   CDD; cd17627; REC_OmpR_PrrA-like; 1.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 3.40.50.2300; -; 1.
DR   Gene3D; 6.10.250.690; -; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; REGULATOR OF RPOS; 1.
DR   PANTHER; PTHR48111:SF22; TRANSCRIPTIONAL REGULATORY PROTEIN CIAR; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; CheY-like; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Stress response; Transcription;
KW   Transcription regulation; Two-component regulatory system; Virulence.
FT   CHAIN           1..232
FT                   /note="Response regulator MprA"
FT                   /id="PRO_0000308429"
FT   DOMAIN          4..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        131..229
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         48
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   232 AA;  26041 MW;  EF0853ACA7D3C835 CRC64;
     MSVRILVVDD DRAVRESLRR SLSFNGYTVE LAHDGVEALE MIASDRPDAL VLDVMMPRLD
     GLEVCRQLRS TGDDLPILVL TARDSVSERV AGLDAGADDY LPKPFALEEL LARMRALLRR
     TKLEDDEAAD SVAMTFSDLS LDPVTREVTR GARRISLTRT EFALLEMLIA NPRRVLTRSR
     ILEEVWGFDF PTSGNALEVY VGYLRRKTEA EGEPRLIHTV RGVGYVLRET PP
//