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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 4011D-inositol 3-phosphateUniRule annotation
Binding sitei54 – 541UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei109 – 10911D-inositol 3-phosphateUniRule annotation
Binding sitei142 – 14211D-inositol 3-phosphateUniRule annotation
Binding sitei166 – 16611D-inositol 3-phosphateUniRule annotation
Binding sitei186 – 18611D-inositol 3-phosphateUniRule annotation
Binding sitei260 – 2601UDP-GlcNAcUniRule annotation
Binding sitei265 – 2651UDP-GlcNAcUniRule annotation
Binding sitei318 – 3181UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi327 – 3271Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi328 – 3281Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi330 – 3301Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei340 – 3401UDP-GlcNAcUniRule annotation
Binding sitei348 – 3481UDP-GlcNAcUniRule annotation
Metal bindingi354 – 3541MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MUL_4556
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000765 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463D-inositol 3-phosphate glycosyltransferasePRO_0000400144Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0PVZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni46 – 472UDP-GlcNAc bindingUniRule annotation
Regioni51 – 5661D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0PVZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAGAGAAGE SCKDDGVRPD DVSRLTADAV VRRVALLAVH TSPLAQPGTG
60 70 80 90 100
DAGGMNVYVL QSALHLAKRG IEVEIFTRAT ASADPPVVRV APGVLVRNVV
110 120 130 140 150
AGPFEGLDKY DLPTQLCAFA AGVLRAEAAH EPGHYDIVHS HYWLSGQVGW
160 170 180 190 200
LARDRWAVPL VHTAHTLAAV KNAALANGDA PEPPLRTVGE QQVVDEADRL
210 220 230 240 250
IVNTDDEAKQ LISIHHADPA RIDVVHPGVD LEVFRPGDRQ QARTALGLRP
260 270 280 290 300
EEKVVAFVGR IQPLKAPDIV LRAVAKLPGV RIIVAGGPSG SGLASPDGLA
310 320 330 340 350
QLADELGIAE RVTFLPPQSR TDLARVFHAV DLVAIPSYSE SFGLVAVEAQ
360 370 380 390 400
ACGTRVVAAA VGGLPVAVRD GVSGTLVSGH DVDQWAAAID GLLRSNAGAQ
410 420 430 440 450
GALMSRAAAE HAATFSWENT TDALLASYRR AIGDFTAGRR RKVRDPVAAR
460
KPRRWTARRG VGA
Length:463
Mass (Da):48,710
Last modified:January 9, 2007 - v1
Checksum:i3AECAE08081DB2DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL06510.1.

Genome annotation databases

EnsemblBacteriaiABL06510; ABL06510; MUL_4556.
KEGGimul:MUL_4556.
PATRICi18176949. VBIMycUlc37413_5275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL06510.1.

3D structure databases

ProteinModelPortaliA0PVZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL06510; ABL06510; MUL_4556.
KEGGimul:MUL_4556.
PATRICi18176949. VBIMycUlc37413_5275.

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
    Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
    , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
    Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Agy99.

Entry informationi

Entry nameiMSHA_MYCUA
AccessioniPrimary (citable) accession number: A0PVZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 9, 2007
Last modified: May 11, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.