A0PVU7 (KGD_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoglutarate decarboxylase
EC=4.1.1.71

Alternative name(s):
2-oxoglutarate carboxy-lyase
Alpha-ketoglutarate decarboxylase

Gene names

Name:	kgd
Ordered Locus Names:	MUL_4500

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	1238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an oxidative decarboxylation from five-carbon 2-oxoglutarate (alpha-ketoglutarate) to four-carbon succinate semialdehyde By similarity.
Catalytic activity	2-oxoglutarate = succinate semialdehyde + CO2.
Cofactor	Magnesium By similarity. Thiamine pyrophosphate By similarity.
Sequence similarities	Belongs to the 2-oxoacid dehydrogenase family. Kgd subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Domain	Coiled coil
Ligand	Magnesium Thiamine pyrophosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-oxoglutarate decarboxylase activity Inferred from electronic annotation. Source: EC oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1238	1238	2-oxoglutarate decarboxylase		PRO_0000310724
Regions
	Coiled coil	794 – 824	31	Potential
	Compositional bias	48 – 119	72	Ala-rich

Sequences

	Sequence		Length	Mass (Da)
	A0PVU7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: B9A7DB3108194D2A	FASTA	1,238	136,742
	10 20 30 40 50 60 MANISSPFGQ NEWLVEEMYR KFRDDPSSVD PSWHEFLVDY NPESTQEATE PAVVKPAAAP 70 80 90 100 110 120 AKPAPAPAPA KPAAGPPAAG NGSPAAAPSA KPAAAPAKAP APPPAEGDEM QVLRGAAAAV 130 140 150 160 170 180 VKNMSASLDV PTATSVRAVP AKLLIDNRIV INNQLKRNRG GKISFTHLLG YALVQAVKKF 190 200 210 220 230 240 PNMNRHYLDV DGKPNAVTPA HTNLGLAIDL QGKDGKRALV VAGIKRCETM RFAQFVTAYE 250 260 270 280 290 300 DIVRRARDGK LTAEDFSGVT ISLTNPGTIG TVHSVPRLMA GQGAIIGVGA MEYPAEFQGA 310 320 330 340 350 360 SEERIAELGI GKLITLTSTY DHRIIQGAES GDFLRTIHQM LLADEFWDEI FRELSIPYLP 370 380 390 400 410 420 VRWRPDNPDS IVDKNARIIE LIAAYRNRGH LMADIDPLRL DKTRFRSHPD LDVCTHGLTL 430 440 450 460 470 480 WDLDRSFKVG GCFAGPQNMK LRDVLSILRD TYCRHVGVEY THILEPEQQQ WLQQRVEAKH 490 500 510 520 530 540 VKPTVAQQKY VLSKLNAAEA FETFLQTKYV GQKRFSLEGA ESVIPMMDAA IDQCAEYGLD 550 560 570 580 590 600 EVVIGMPHRG RLNVLANIVG KPYSQIFSEF EGNLNPSQAH GSGDVKYHLG ATGVYLQMFG 610 620 630 640 650 660 DNDIQVSLTA NPSHLEAVDP VLEGLVRAKQ DLLEHGETDT ENQRAFSVVP MMLHGDAAFA 670 680 690 700 710 720 GQGVVAETLN LANLPGYRVG GTIHIIVNNQ IGFTTAPEYS RSTEYCTDVA KTIGAPIFHV 730 740 750 760 770 780 NGDDPEACVW VARLAVDFRQ RFNKDVIIDM LCYRRRGHNE GDDPSMTNPR MYDVVDTKRG 790 800 810 820 830 840 VRKSYTEALI GRGDISIKEA EDALRDYQGQ LEQVFNEVRE LEKHGAQPSE SVESDQMIPA 850 860 870 880 890 900 GLATAVDKSL LARIGDAFLA VPDGFTTHPR VQPVLEKRRE MAYEGKIDWA FAELLALGSL 910 920 930 940 950 960 VAEGKLVRFS GQDTRRGTFS QRHSVIIDRH TREEFTPLQL LTTNKDGSPT GGKFLVYDSP 970 980 990 1000 1010 1020 LSEYAAVGFE YGYTVGNPDA VVLWEAQFGD FVNGAQSIID EFISSGEAKW GQLSNVVLLL 1030 1040 1050 1060 1070 1080 PHGHEGQGPD HTSGRIERFL QLWAEGSMTI AMPSTPSNYF HLLRRHALDG IQRPLIVFTP 1090 1100 1110 1120 1130 1140 KSMLRNKAAV SDIKDFTEIK FRSVLEEPTY EDGVGDRNLV NRILLTSGKI YYEMVARKAK 1150 1160 1170 1180 1190 1200 DKREDVAIVR VEQLAPLPRR RLRETLDRYP NAKEFFWVQE EPANQGAWPR FGLELPELLP 1210 1220 1230 EKLSGVKRIS RRAMSAPSSG SSKVHAVEQQ EILDTAFG

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL06466.1.
RefSeq	YP_907937.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PVU7.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PVU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000073519; EBMYCP00000071524; EBMYCG00000073514.
GeneID	4549927.
GenomeReviews	Gene locus MUL_4500 in contig CP000325_GR.
KEGG	mul:MUL_4500.
PATRIC	18176818. VBIMycUlc37413_5210.
Organism-specific databases
GenoList	MUL_4500.
CMR	Search...
Phylogenomic databases
eggNOG	COG0567.
GeneTree	EBGT00050000014858.
HOGENOM	HBG289950.
OMA	EGDEPAF.
PhylomeDB	A0PVU7.
ProtClustDB	PRK12270.
Family and domain databases
InterPro	IPR001078. 2-oxoacid_DH_actylTfrase. IPR011603. 2oxoglutarate_DH_E1. IPR023213. CAT-like_dom. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
Gene3D	G3DSA:3.30.559.10. CAT-like_dom. 1 hit.
KO	K01616.
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00198. 2-oxoacid_dh. 1 hit. PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KGD_MYCUA

Accession

Primary (citable) accession number: A0PVU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program